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Rubisco activase activity assays

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<mark>Journal publication date</mark>7/03/2011
<mark>Journal</mark>Methods in Molecular Biology
Volume684
Number of pages8
Pages (from-to)375-382
Publication StatusPublished
<mark>Original language</mark>English

Abstract

Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) activase functions as a mechano-chemical motor protein using the energy from ATP hydrolysis to contort the structure of its target protein, Rubisco. This action modulates the activation state of Rubisco by removing tightly-bound inhibitory sugar-phosphates from Rubisco's catalytic sites, thereby restoring the sites to catalytic competence. This chapter reports methods developed for assaying the two activities of Rubisco activase: ATP hydrolysis and Rubisco activation.