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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Rubisco and carbon-concentrating mechanism co-evolution across chlorophyte and streptophyte green algae
AU - Goudet, Myriam
AU - Orr, Douglas
AU - Melkonian, Michael
AU - Muller, Karin
AU - Meyer, Moritz
AU - Carmo-Silva, Elizabete
AU - Griffiths, Howard
PY - 2020/8/1
Y1 - 2020/8/1
N2 - Green algae expressing a carbon‐concentrating mechanism (CCM) are usually associated with a Rubisco‐containing micro‐compartment, the pyrenoid. A link between the small subunit (SSU) of Rubisco and pyrenoid formation in Chlamydomonas reinhardtii has previously suggested that specific RbcS residues could explain pyrenoid occurrence in green algae.A phylogeny of RbcS was used to compare the protein sequence and CCM distribution across the green algae and positive selection in RbcS was estimated. For six streptophyte algae, Rubisco catalytic properties, affinity for CO2 uptake (K0.5), carbon isotope discrimination (δ13C) and pyrenoid morphology were compared.The length of the βA–βB loop in RbcS provided a phylogenetic marker discriminating chlorophyte from streptophyte green algae. Rubisco kinetic properties in streptophyte algae have responded to the extent of inducible CCM activity, as indicated by changes in inorganic carbon uptake affinity, δ13C and pyrenoid ultrastructure between high and low CO2 conditions for growth.We conclude that the Rubisco catalytic properties found in streptophyte algae have coevolved and reflect the strength of any CCM or degree of pyrenoid leakiness, and limitations to inorganic carbon in the aquatic habitat, whereas Rubisco in extant land plants reflects more recent selective pressures associated with improved diffusive supply of the terrestrial environment.
AB - Green algae expressing a carbon‐concentrating mechanism (CCM) are usually associated with a Rubisco‐containing micro‐compartment, the pyrenoid. A link between the small subunit (SSU) of Rubisco and pyrenoid formation in Chlamydomonas reinhardtii has previously suggested that specific RbcS residues could explain pyrenoid occurrence in green algae.A phylogeny of RbcS was used to compare the protein sequence and CCM distribution across the green algae and positive selection in RbcS was estimated. For six streptophyte algae, Rubisco catalytic properties, affinity for CO2 uptake (K0.5), carbon isotope discrimination (δ13C) and pyrenoid morphology were compared.The length of the βA–βB loop in RbcS provided a phylogenetic marker discriminating chlorophyte from streptophyte green algae. Rubisco kinetic properties in streptophyte algae have responded to the extent of inducible CCM activity, as indicated by changes in inorganic carbon uptake affinity, δ13C and pyrenoid ultrastructure between high and low CO2 conditions for growth.We conclude that the Rubisco catalytic properties found in streptophyte algae have coevolved and reflect the strength of any CCM or degree of pyrenoid leakiness, and limitations to inorganic carbon in the aquatic habitat, whereas Rubisco in extant land plants reflects more recent selective pressures associated with improved diffusive supply of the terrestrial environment.
KW - carbon‐concentrating mechanism (CCM)
KW - green algae
KW - photosynthesis
KW - pyrenoid
KW - Rubisco
KW - streptophyte algae
U2 - 10.1111/nph.16577
DO - 10.1111/nph.16577
M3 - Journal article
VL - 227
SP - 810
EP - 823
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 3
ER -