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Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera Culicidae)

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Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera Culicidae). / Jariyapan, Narissara; Choochote, Wej; Jitpakdi, Atchariya; Harnnoi, Thasaneeya; Siriyasatein, Padet; Wilkinson, Mark C; Junkum, Anuluck; Bates, Paul A.

In: Revista do Instituto de Medicina Tropical de São Paulo, Vol. 49, No. 1, 01.2007, p. 5-10.

Research output: Contribution to journalJournal article

Harvard

Jariyapan, N, Choochote, W, Jitpakdi, A, Harnnoi, T, Siriyasatein, P, Wilkinson, MC, Junkum, A & Bates, PA 2007, 'Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera Culicidae)', Revista do Instituto de Medicina Tropical de São Paulo, vol. 49, no. 1, pp. 5-10. <http://www.scielo.br/pdf/rimtsp/v49n1/a02v49n1.pdf>

APA

Jariyapan, N., Choochote, W., Jitpakdi, A., Harnnoi, T., Siriyasatein, P., Wilkinson, M. C., Junkum, A., & Bates, P. A. (2007). Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera Culicidae). Revista do Instituto de Medicina Tropical de São Paulo, 49(1), 5-10. http://www.scielo.br/pdf/rimtsp/v49n1/a02v49n1.pdf

Vancouver

Jariyapan N, Choochote W, Jitpakdi A, Harnnoi T, Siriyasatein P, Wilkinson MC et al. Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera Culicidae). Revista do Instituto de Medicina Tropical de São Paulo. 2007 Jan;49(1):5-10.

Author

Jariyapan, Narissara ; Choochote, Wej ; Jitpakdi, Atchariya ; Harnnoi, Thasaneeya ; Siriyasatein, Padet ; Wilkinson, Mark C ; Junkum, Anuluck ; Bates, Paul A. / Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera Culicidae). In: Revista do Instituto de Medicina Tropical de São Paulo. 2007 ; Vol. 49, No. 1. pp. 5-10.

Bibtex

@article{6370a732d4614045a378116fe283ae44,
title = "Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera Culicidae)",
abstract = "Salivary gland proteins of the human malaria vector, Anopheles dirus B were determined and analyzed. The amount of salivary gland proteins in mosquitoes aged between 3--10 days was approximately 1.08 +/- 0.04 microg/female and 0.1 +/- 0.05 microg/male. The salivary glands of both sexes displayed the same morphological organization as that of other anopheline mosquitoes. In females, apyrase accumulated in the distal regions, whereas alpha-glucosidase was found in the proximal region of the lateral lobes. This differential distribution of the analyzed enzymes reflects specialization of different regions for sugar and blood feeding. SDS-PAGE analysis revealed that at least seven major proteins were found in the female salivary glands, of which each morphological region contained different major proteins. Similar electrophoretic protein profiles were detected comparing unfed and blood-fed mosquitoes, suggesting that there is no specific protein induced by blood. Two-dimensional polyacrylamide gel analysis showed the most abundant salivary gland protein, with a molecular mass of approximately 35 kilodaltons and an isoelectric point of approximately 4.0. These results provide basic information that would lead to further study on the role of salivary proteins of An. dirus B in disease transmission and hematophagy.",
keywords = "Anopheles, Salivary gland, Malaria , Apyrase , Alpha-glucosidase",
author = "Narissara Jariyapan and Wej Choochote and Atchariya Jitpakdi and Thasaneeya Harnnoi and Padet Siriyasatein and Wilkinson, {Mark C} and Anuluck Junkum and Bates, {Paul A}",
year = "2007",
month = jan,
language = "English",
volume = "49",
pages = "5--10",
journal = "Revista do Instituto de Medicina Tropical de S{\~a}o Paulo",
issn = "0036-4665",
publisher = "Instituto de Medicina Tropical de Sao Paulo",
number = "1",

}

RIS

TY - JOUR

T1 - Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera Culicidae)

AU - Jariyapan, Narissara

AU - Choochote, Wej

AU - Jitpakdi, Atchariya

AU - Harnnoi, Thasaneeya

AU - Siriyasatein, Padet

AU - Wilkinson, Mark C

AU - Junkum, Anuluck

AU - Bates, Paul A

PY - 2007/1

Y1 - 2007/1

N2 - Salivary gland proteins of the human malaria vector, Anopheles dirus B were determined and analyzed. The amount of salivary gland proteins in mosquitoes aged between 3--10 days was approximately 1.08 +/- 0.04 microg/female and 0.1 +/- 0.05 microg/male. The salivary glands of both sexes displayed the same morphological organization as that of other anopheline mosquitoes. In females, apyrase accumulated in the distal regions, whereas alpha-glucosidase was found in the proximal region of the lateral lobes. This differential distribution of the analyzed enzymes reflects specialization of different regions for sugar and blood feeding. SDS-PAGE analysis revealed that at least seven major proteins were found in the female salivary glands, of which each morphological region contained different major proteins. Similar electrophoretic protein profiles were detected comparing unfed and blood-fed mosquitoes, suggesting that there is no specific protein induced by blood. Two-dimensional polyacrylamide gel analysis showed the most abundant salivary gland protein, with a molecular mass of approximately 35 kilodaltons and an isoelectric point of approximately 4.0. These results provide basic information that would lead to further study on the role of salivary proteins of An. dirus B in disease transmission and hematophagy.

AB - Salivary gland proteins of the human malaria vector, Anopheles dirus B were determined and analyzed. The amount of salivary gland proteins in mosquitoes aged between 3--10 days was approximately 1.08 +/- 0.04 microg/female and 0.1 +/- 0.05 microg/male. The salivary glands of both sexes displayed the same morphological organization as that of other anopheline mosquitoes. In females, apyrase accumulated in the distal regions, whereas alpha-glucosidase was found in the proximal region of the lateral lobes. This differential distribution of the analyzed enzymes reflects specialization of different regions for sugar and blood feeding. SDS-PAGE analysis revealed that at least seven major proteins were found in the female salivary glands, of which each morphological region contained different major proteins. Similar electrophoretic protein profiles were detected comparing unfed and blood-fed mosquitoes, suggesting that there is no specific protein induced by blood. Two-dimensional polyacrylamide gel analysis showed the most abundant salivary gland protein, with a molecular mass of approximately 35 kilodaltons and an isoelectric point of approximately 4.0. These results provide basic information that would lead to further study on the role of salivary proteins of An. dirus B in disease transmission and hematophagy.

KW - Anopheles

KW - Salivary gland

KW - Malaria

KW - Apyrase

KW - Alpha-glucosidase

M3 - Journal article

C2 - 17384813

VL - 49

SP - 5

EP - 10

JO - Revista do Instituto de Medicina Tropical de São Paulo

JF - Revista do Instituto de Medicina Tropical de São Paulo

SN - 0036-4665

IS - 1

ER -