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Secretory form of Alzheimer amyloid precursor protein 695 in human brain lacks β/A4 amyloid immunoreactivity

Research output: Contribution to journalJournal article

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  • F Kametani
  • K Tanaka
  • T Ishii
  • S Ikeda
  • H E Kennedy
  • D Allsop
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<mark>Journal publication date</mark>15/03/1993
<mark>Journal</mark>Biochemical and Biophysical Research Communications
Issue number2
Volume191
Number of pages7
Pages (from-to)392-398
Publication statusPublished
Original languageEnglish

Abstract

It is not clear how Alzheimer amyloid precursor proteins (APP) are metabolized in the brain itself. Secretory forms of APP in a phosphate buffer-soluble fraction were purified from post-mortem human brain by heparin-affinity and ion-exchange chromatography and analyzed by N-terminal amino acid sequencing and SDS polyacrylamide gel electrophoresis/immunoblotting. We found apparently similar multi-isoforms of secretory APP (at 93-97, 105-112 and 123 KDa) to those that we have described recently in cerebrospinal fluid. Antisera to the initial part of the beta/A4 sequence labelled only those bands that were found to react with antiserum to the Kunitz-type inhibitor insert of APP, suggesting that beta/A4 amyloid may be generated specifically from APP-695.