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Seeing ‘cool’ and ‘hot’—infrared thermography as a tool for non-invasive, high-throughput screening of Arabidopsis guard cell signalling mutants.

Research output: Contribution to journalJournal article

Published

Journal publication date05/2004
JournalJournal of Experimental Botany
Journal number400
Volume55
Number of pages7
Pages1187-1193
Original languageEnglish

Abstract

The use of Arabidopsis mutants defective in abscisic acid (ABA) perception has been instrumental in the understanding of stomatal function, in particular, ABA signalling in guard cells. The considerable attention devoted to ABA signalling in guard cells is due in part to (1) the fundamental role of ABA in drought stress and (2) the use of a screening protocol based on the sensitivity of seed germination to ABA. Such a screen has facilitated the isolation of ABA signalling mutants with genetic lesions that exert pleiotropic effects at the whole plant level. As such, there is a requirement for new approaches to complement the seed germination screen. The recent advances made in the use of infrared thermography as a non-invasive, high-throughput tool are reviewed here and the versatility of this technique for screening Arabidopsis defective in stomatal regulation is highlighted.