Seeing ‘cool’ and ‘hot’—infrared thermography as a tool for non-invasive, high-throughput screening of Arabidopsis guard cell signalling mutants.

Lancaster Environment Centre

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https://doi.org/10.1093/jxb/erh135
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Keywords

Arabidopsis, drought, EMS mutants, infrared thermography, low humidity.

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Seeing ‘cool’ and ‘hot’—infrared thermography as a tool for non-invasive, high-throughput screening of Arabidopsis guard cell signalling mutants. / Wang, Yibing; Holroyd, Geoffrey; Hetherington, Alistair et al.
In: Journal of Experimental Botany, Vol. 55, No. 400, 05.2004, p. 1187-1193.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{51ed519a13ec4d1289ce8aae0e76e749,

title = "Seeing {\textquoteleft}cool{\textquoteright} and {\textquoteleft}hot{\textquoteright}—infrared thermography as a tool for non-invasive, high-throughput screening of Arabidopsis guard cell signalling mutants.",

abstract = "The use of Arabidopsis mutants defective in abscisic acid (ABA) perception has been instrumental in the understanding of stomatal function, in particular, ABA signalling in guard cells. The considerable attention devoted to ABA signalling in guard cells is due in part to (1) the fundamental role of ABA in drought stress and (2) the use of a screening protocol based on the sensitivity of seed germination to ABA. Such a screen has facilitated the isolation of ABA signalling mutants with genetic lesions that exert pleiotropic effects at the whole plant level. As such, there is a requirement for new approaches to complement the seed germination screen. The recent advances made in the use of infrared thermography as a non-invasive, high-throughput tool are reviewed here and the versatility of this technique for screening Arabidopsis defective in stomatal regulation is highlighted.",

keywords = "Arabidopsis, drought, EMS mutants, infrared thermography, low humidity.",

author = "Yibing Wang and Geoffrey Holroyd and Alistair Hetherington and Ng, {Carl K-Y.}",

year = "2004",

month = may,

doi = "10.1093/jxb/erh135",

language = "English",

volume = "55",

pages = "1187--1193",

journal = "Journal of Experimental Botany",

issn = "1460-2431",

publisher = "OXFORD UNIV PRESS",

number = "400",

}

RIS

TY - JOUR

T1 - Seeing ‘cool’ and ‘hot’—infrared thermography as a tool for non-invasive, high-throughput screening of Arabidopsis guard cell signalling mutants.

AU - Wang, Yibing

AU - Holroyd, Geoffrey

AU - Hetherington, Alistair

AU - Ng, Carl K-Y.

PY - 2004/5

Y1 - 2004/5

N2 - The use of Arabidopsis mutants defective in abscisic acid (ABA) perception has been instrumental in the understanding of stomatal function, in particular, ABA signalling in guard cells. The considerable attention devoted to ABA signalling in guard cells is due in part to (1) the fundamental role of ABA in drought stress and (2) the use of a screening protocol based on the sensitivity of seed germination to ABA. Such a screen has facilitated the isolation of ABA signalling mutants with genetic lesions that exert pleiotropic effects at the whole plant level. As such, there is a requirement for new approaches to complement the seed germination screen. The recent advances made in the use of infrared thermography as a non-invasive, high-throughput tool are reviewed here and the versatility of this technique for screening Arabidopsis defective in stomatal regulation is highlighted.

AB - The use of Arabidopsis mutants defective in abscisic acid (ABA) perception has been instrumental in the understanding of stomatal function, in particular, ABA signalling in guard cells. The considerable attention devoted to ABA signalling in guard cells is due in part to (1) the fundamental role of ABA in drought stress and (2) the use of a screening protocol based on the sensitivity of seed germination to ABA. Such a screen has facilitated the isolation of ABA signalling mutants with genetic lesions that exert pleiotropic effects at the whole plant level. As such, there is a requirement for new approaches to complement the seed germination screen. The recent advances made in the use of infrared thermography as a non-invasive, high-throughput tool are reviewed here and the versatility of this technique for screening Arabidopsis defective in stomatal regulation is highlighted.

KW - Arabidopsis

KW - drought

KW - EMS mutants

KW - infrared thermography

KW - low humidity.

U2 - 10.1093/jxb/erh135

DO - 10.1093/jxb/erh135

M3 - Journal article

VL - 55

SP - 1187

EP - 1193

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 1460-2431

IS - 400

ER -

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