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Selenium- or quercetin-induced retardation of DNA synthesis in primary prostate cells occurs in the presence of a concomitant reduction in androgen-receptor activity.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Selenium- or quercetin-induced retardation of DNA synthesis in primary prostate cells occurs in the presence of a concomitant reduction in androgen-receptor activity. / Morris, Jonathan D.; Pramanik, Rashida; Zhang, Xin et al.
In: Cancer Letters, Vol. 239, No. 1, 28.07.2006, p. 111-122.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Morris, JD, Pramanik, R, Zhang, X, Carey, AM, Ragavan, N, Martin, FL & Muir, GH 2006, 'Selenium- or quercetin-induced retardation of DNA synthesis in primary prostate cells occurs in the presence of a concomitant reduction in androgen-receptor activity.', Cancer Letters, vol. 239, no. 1, pp. 111-122. https://doi.org/10.1016/j.canlet.2005.07.037

APA

Morris, J. D., Pramanik, R., Zhang, X., Carey, A. M., Ragavan, N., Martin, F. L., & Muir, G. H. (2006). Selenium- or quercetin-induced retardation of DNA synthesis in primary prostate cells occurs in the presence of a concomitant reduction in androgen-receptor activity. Cancer Letters, 239(1), 111-122. https://doi.org/10.1016/j.canlet.2005.07.037

Vancouver

Morris JD, Pramanik R, Zhang X, Carey AM, Ragavan N, Martin FL et al. Selenium- or quercetin-induced retardation of DNA synthesis in primary prostate cells occurs in the presence of a concomitant reduction in androgen-receptor activity. Cancer Letters. 2006 Jul 28;239(1):111-122. doi: 10.1016/j.canlet.2005.07.037

Author

Morris, Jonathan D. ; Pramanik, Rashida ; Zhang, Xin et al. / Selenium- or quercetin-induced retardation of DNA synthesis in primary prostate cells occurs in the presence of a concomitant reduction in androgen-receptor activity. In: Cancer Letters. 2006 ; Vol. 239, No. 1. pp. 111-122.

Bibtex

@article{4c9d88f5ee2c40d48edb5a6c05bdc7a2,
title = "Selenium- or quercetin-induced retardation of DNA synthesis in primary prostate cells occurs in the presence of a concomitant reduction in androgen-receptor activity.",
abstract = "Prostate cancer (CaP) is the most common male malignancy in the Western world. Selenium or quercetin may down-regulate prostate-cell proliferation in immortalised cells (e.g. androgen-responsive LNCaP cells). However, whether such effects are apparent in primary prostate epithelial cells (PECs) remains to be examined. Following surgical resection, primary PECs isolated from tissues (n=10 patients) were cultured in the presence or absence of selenium, selenomethionine or quercetin. Tissues from a minimum of three patients were used to generate cell preparations that were cultured independently for the purposes of the experimental analysis of each test agent. These agents were also examined in LNCaP cells. DNA synthesis was assessed by the percentage of PECs or LNCaP cells that incorporated 5-bromo-2-deoxyuridine (BrdU) into DNA. All three test agents induced a dose-related reduction in the percentage of PECs or LNCaP cells labelled with BrdU. In LNCaP cells transfected with an androgen-receptor (AR)-reporter gene coupled to luciferase, selenomethionine or quercetin reduced AR activity. Chemoprevention may retard DNA synthesis in short-term primary PECs and expression of AR-inducible elements may be a concomitant factor.",
keywords = "Androgen receptor, Luciferase-reporter gene, LNCaP cells, Prostate cancer, Prostate epithelial cells, Quercetin, Selenium",
author = "Morris, {Jonathan D.} and Rashida Pramanik and Xin Zhang and Carey, {Anne Marie} and Narasimhan Ragavan and Martin, {Francis L.} and Muir, {Gordon H.}",
year = "2006",
month = jul,
day = "28",
doi = "10.1016/j.canlet.2005.07.037",
language = "English",
volume = "239",
pages = "111--122",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Selenium- or quercetin-induced retardation of DNA synthesis in primary prostate cells occurs in the presence of a concomitant reduction in androgen-receptor activity.

AU - Morris, Jonathan D.

AU - Pramanik, Rashida

AU - Zhang, Xin

AU - Carey, Anne Marie

AU - Ragavan, Narasimhan

AU - Martin, Francis L.

AU - Muir, Gordon H.

PY - 2006/7/28

Y1 - 2006/7/28

N2 - Prostate cancer (CaP) is the most common male malignancy in the Western world. Selenium or quercetin may down-regulate prostate-cell proliferation in immortalised cells (e.g. androgen-responsive LNCaP cells). However, whether such effects are apparent in primary prostate epithelial cells (PECs) remains to be examined. Following surgical resection, primary PECs isolated from tissues (n=10 patients) were cultured in the presence or absence of selenium, selenomethionine or quercetin. Tissues from a minimum of three patients were used to generate cell preparations that were cultured independently for the purposes of the experimental analysis of each test agent. These agents were also examined in LNCaP cells. DNA synthesis was assessed by the percentage of PECs or LNCaP cells that incorporated 5-bromo-2-deoxyuridine (BrdU) into DNA. All three test agents induced a dose-related reduction in the percentage of PECs or LNCaP cells labelled with BrdU. In LNCaP cells transfected with an androgen-receptor (AR)-reporter gene coupled to luciferase, selenomethionine or quercetin reduced AR activity. Chemoprevention may retard DNA synthesis in short-term primary PECs and expression of AR-inducible elements may be a concomitant factor.

AB - Prostate cancer (CaP) is the most common male malignancy in the Western world. Selenium or quercetin may down-regulate prostate-cell proliferation in immortalised cells (e.g. androgen-responsive LNCaP cells). However, whether such effects are apparent in primary prostate epithelial cells (PECs) remains to be examined. Following surgical resection, primary PECs isolated from tissues (n=10 patients) were cultured in the presence or absence of selenium, selenomethionine or quercetin. Tissues from a minimum of three patients were used to generate cell preparations that were cultured independently for the purposes of the experimental analysis of each test agent. These agents were also examined in LNCaP cells. DNA synthesis was assessed by the percentage of PECs or LNCaP cells that incorporated 5-bromo-2-deoxyuridine (BrdU) into DNA. All three test agents induced a dose-related reduction in the percentage of PECs or LNCaP cells labelled with BrdU. In LNCaP cells transfected with an androgen-receptor (AR)-reporter gene coupled to luciferase, selenomethionine or quercetin reduced AR activity. Chemoprevention may retard DNA synthesis in short-term primary PECs and expression of AR-inducible elements may be a concomitant factor.

KW - Androgen receptor

KW - Luciferase-reporter gene

KW - LNCaP cells

KW - Prostate cancer

KW - Prostate epithelial cells

KW - Quercetin

KW - Selenium

U2 - 10.1016/j.canlet.2005.07.037

DO - 10.1016/j.canlet.2005.07.037

M3 - Journal article

VL - 239

SP - 111

EP - 122

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -