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Sequences of complete human cytomegalovirus genomes from infected cell cultures and clinical specimens

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Sequences of complete human cytomegalovirus genomes from infected cell cultures and clinical specimens. / Cunningham, Charles; Gatherer, Derek; Hilfrich, Birgitta; Baluchova, Katarina; Dargan, Derrick J.; Thomson, Marian; Griffiths, Paul D.; Wilkinson, Gavin W. G.; Schulz, Thomas F.; Davison, Andrew J.

In: Journal of General Virology, Vol. 91, No. 3, 01.03.2010, p. 605-615.

Research output: Contribution to journalJournal article

Harvard

Cunningham, C, Gatherer, D, Hilfrich, B, Baluchova, K, Dargan, DJ, Thomson, M, Griffiths, PD, Wilkinson, GWG, Schulz, TF & Davison, AJ 2010, 'Sequences of complete human cytomegalovirus genomes from infected cell cultures and clinical specimens', Journal of General Virology, vol. 91, no. 3, pp. 605-615. https://doi.org/10.1099/vir.0.015891-0

APA

Cunningham, C., Gatherer, D., Hilfrich, B., Baluchova, K., Dargan, D. J., Thomson, M., Griffiths, P. D., Wilkinson, G. W. G., Schulz, T. F., & Davison, A. J. (2010). Sequences of complete human cytomegalovirus genomes from infected cell cultures and clinical specimens. Journal of General Virology, 91(3), 605-615. https://doi.org/10.1099/vir.0.015891-0

Vancouver

Cunningham C, Gatherer D, Hilfrich B, Baluchova K, Dargan DJ, Thomson M et al. Sequences of complete human cytomegalovirus genomes from infected cell cultures and clinical specimens. Journal of General Virology. 2010 Mar 1;91(3):605-615. https://doi.org/10.1099/vir.0.015891-0

Author

Cunningham, Charles ; Gatherer, Derek ; Hilfrich, Birgitta ; Baluchova, Katarina ; Dargan, Derrick J. ; Thomson, Marian ; Griffiths, Paul D. ; Wilkinson, Gavin W. G. ; Schulz, Thomas F. ; Davison, Andrew J. / Sequences of complete human cytomegalovirus genomes from infected cell cultures and clinical specimens. In: Journal of General Virology. 2010 ; Vol. 91, No. 3. pp. 605-615.

Bibtex

@article{4ab2578117b04af285df695af0e2c0af,
title = "Sequences of complete human cytomegalovirus genomes from infected cell cultures and clinical specimens",
abstract = "We have assessed two approaches to sequencing complete human cytomegalovirus (HCMV) genomes (236 kbp) in DNA extracted from infected cell cultures (strains 3157, HAN13, HAN20 and HAN38) or clinical specimens (strains JP and 3301). The first approach involved amplifying genomes from the DNA samples as overlapping PCR products, sequencing these by the Sanger method, acquiring reads from a capillary instrument and assembling these using the Staden programs. The second approach involved generating sequence data from the DNA samples by using an Illumina Genome Analyzer (IGA), processing the filtered reads by reference-independent (de novo) assembly, utilizing the resulting sequence to direct reference-dependent assembly of the same data and finishing by limited PCR sequencing. Both approaches were successful. In particular, the investigation demonstrated the utility of IGA data for efficiently sequencing genomes from clinical samples containing as little as 3 % HCMV DNA. Analysis of the genome sequences obtained showed that each of the strains grown in cell culture was a mutant. Certain of the mutations were shared among strains from independent clinical sources, thus suggesting that they may have arisen in a common ancestor during natural infection. Moreover, one of the strains (JP) sequenced directly from a clinical specimen was mutated in two genes, one of which encodes a proposed immune-evasion function, viral interleukin-10. These observations imply that HCMV mutants exist in human infections.",
author = "Charles Cunningham and Derek Gatherer and Birgitta Hilfrich and Katarina Baluchova and Dargan, {Derrick J.} and Marian Thomson and Griffiths, {Paul D.} and Wilkinson, {Gavin W. G.} and Schulz, {Thomas F.} and Davison, {Andrew J.}",
year = "2010",
month = mar,
day = "1",
doi = "10.1099/vir.0.015891-0",
language = "English",
volume = "91",
pages = "605--615",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "3",

}

RIS

TY - JOUR

T1 - Sequences of complete human cytomegalovirus genomes from infected cell cultures and clinical specimens

AU - Cunningham, Charles

AU - Gatherer, Derek

AU - Hilfrich, Birgitta

AU - Baluchova, Katarina

AU - Dargan, Derrick J.

AU - Thomson, Marian

AU - Griffiths, Paul D.

AU - Wilkinson, Gavin W. G.

AU - Schulz, Thomas F.

AU - Davison, Andrew J.

PY - 2010/3/1

Y1 - 2010/3/1

N2 - We have assessed two approaches to sequencing complete human cytomegalovirus (HCMV) genomes (236 kbp) in DNA extracted from infected cell cultures (strains 3157, HAN13, HAN20 and HAN38) or clinical specimens (strains JP and 3301). The first approach involved amplifying genomes from the DNA samples as overlapping PCR products, sequencing these by the Sanger method, acquiring reads from a capillary instrument and assembling these using the Staden programs. The second approach involved generating sequence data from the DNA samples by using an Illumina Genome Analyzer (IGA), processing the filtered reads by reference-independent (de novo) assembly, utilizing the resulting sequence to direct reference-dependent assembly of the same data and finishing by limited PCR sequencing. Both approaches were successful. In particular, the investigation demonstrated the utility of IGA data for efficiently sequencing genomes from clinical samples containing as little as 3 % HCMV DNA. Analysis of the genome sequences obtained showed that each of the strains grown in cell culture was a mutant. Certain of the mutations were shared among strains from independent clinical sources, thus suggesting that they may have arisen in a common ancestor during natural infection. Moreover, one of the strains (JP) sequenced directly from a clinical specimen was mutated in two genes, one of which encodes a proposed immune-evasion function, viral interleukin-10. These observations imply that HCMV mutants exist in human infections.

AB - We have assessed two approaches to sequencing complete human cytomegalovirus (HCMV) genomes (236 kbp) in DNA extracted from infected cell cultures (strains 3157, HAN13, HAN20 and HAN38) or clinical specimens (strains JP and 3301). The first approach involved amplifying genomes from the DNA samples as overlapping PCR products, sequencing these by the Sanger method, acquiring reads from a capillary instrument and assembling these using the Staden programs. The second approach involved generating sequence data from the DNA samples by using an Illumina Genome Analyzer (IGA), processing the filtered reads by reference-independent (de novo) assembly, utilizing the resulting sequence to direct reference-dependent assembly of the same data and finishing by limited PCR sequencing. Both approaches were successful. In particular, the investigation demonstrated the utility of IGA data for efficiently sequencing genomes from clinical samples containing as little as 3 % HCMV DNA. Analysis of the genome sequences obtained showed that each of the strains grown in cell culture was a mutant. Certain of the mutations were shared among strains from independent clinical sources, thus suggesting that they may have arisen in a common ancestor during natural infection. Moreover, one of the strains (JP) sequenced directly from a clinical specimen was mutated in two genes, one of which encodes a proposed immune-evasion function, viral interleukin-10. These observations imply that HCMV mutants exist in human infections.

UR - http://www.scopus.com/inward/record.url?scp=76849105203&partnerID=8YFLogxK

U2 - 10.1099/vir.0.015891-0

DO - 10.1099/vir.0.015891-0

M3 - Journal article

AN - SCOPUS:76849105203

VL - 91

SP - 605

EP - 615

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 3

ER -