TY - JOUR

T1 - Sequential extraction of low concentrations of pyrene and formation of non-extractable residues in sterile and non-sterile soils.

AU - Macleod, Christopher J. A.

AU - Semple, Kirk T.

PY - 2003/11

Y1 - 2003/11

N2 - In this study, temporal changes in the extractability of C-14-pyrene, at native concentrations, were followed in two soils with differing organic matter contents, under sterile and non-sterile conditions over 24 weeks by a sequential solvent extraction scheme. No significant loss of the added C-14-pyrene was observed during the incubation. Significant decreases in methanol:water and n-butanol extractability were observed with increasing soil-pyrene contact time. Significant non-extractable residues were formed in all soils, with the largest increases found in the non-sterile soils. After 8 weeks soil-pyrene contact time, there was a significant increase in the rate and extent of sequestration of pyrene in the biologically active soils. This indicated that the aging of pyrene was initially a physical process, with active microbial communities increasing the rate and extent of residue formation after 8 weeks soil-pyrene contact time. These findings suggest that there is a need for longer term ageing experiments following the role of microbial communities on the formation of solvent non-extractable residues. The humin fraction of the soil organic matter contained the majority of the C-14-pyrene associated activity which was not extractable using the scheme of sequential solvents. Saponification of the soil humin resulted in the release of similar amounts of C-14-pyrene associated activity from sterile and non-sterile soils. Solvent extraction with methanol:water was found to significantly underestimate the bioavailable fraction, whereas n-butanol overestimated the bioavailability of the C-14-pyrene-associated activity when assessed by bacterial mineralization after 24 weeks soil-pyrene contact time. (C) 2003 Elsevier Ltd. All rights reserved.

AB - In this study, temporal changes in the extractability of C-14-pyrene, at native concentrations, were followed in two soils with differing organic matter contents, under sterile and non-sterile conditions over 24 weeks by a sequential solvent extraction scheme. No significant loss of the added C-14-pyrene was observed during the incubation. Significant decreases in methanol:water and n-butanol extractability were observed with increasing soil-pyrene contact time. Significant non-extractable residues were formed in all soils, with the largest increases found in the non-sterile soils. After 8 weeks soil-pyrene contact time, there was a significant increase in the rate and extent of sequestration of pyrene in the biologically active soils. This indicated that the aging of pyrene was initially a physical process, with active microbial communities increasing the rate and extent of residue formation after 8 weeks soil-pyrene contact time. These findings suggest that there is a need for longer term ageing experiments following the role of microbial communities on the formation of solvent non-extractable residues. The humin fraction of the soil organic matter contained the majority of the C-14-pyrene associated activity which was not extractable using the scheme of sequential solvents. Saponification of the soil humin resulted in the release of similar amounts of C-14-pyrene associated activity from sterile and non-sterile soils. Solvent extraction with methanol:water was found to significantly underestimate the bioavailable fraction, whereas n-butanol overestimated the bioavailability of the C-14-pyrene-associated activity when assessed by bacterial mineralization after 24 weeks soil-pyrene contact time. (C) 2003 Elsevier Ltd. All rights reserved.

KW - sequential solvent extraction

KW - bioavailability

KW - non-extractable residues

KW - microbial activity

U2 - 10.1016/S0038-0717(03)00238-4

DO - 10.1016/S0038-0717(03)00238-4

M3 - Journal article

VL - 35

SP - 1443

EP - 1450

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

IS - 11

ER -