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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Siberian Miscanthus sacchariflorus accessions surpass the exceptional chilling tolerance of the most widely cultivated clone of Miscanthus x giganteus
AU - Pignon, Charles P.
AU - Spitz, Idan
AU - Sacks, Erik J.
AU - Jørgensen, Uffe
AU - Kørup, Kirsten
AU - Long, Stephen P.
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Chilling temperatures (0–15°C) inhibit photosynthesis in most C4 grasses, yet photosynthesis is chilling tolerant in the ‘Illinois’ clone of the C4 grass Miscanthus x giganteus, a candidate cellulosic bioenergy crop. M. x giganteus is a hybrid between Miscanthus sacchariflorus and Miscanthus sinensis; therefore chilling-tolerant parent lines might produce hybrids superior to the current clone. Recently a collection of M. sacchariflorus from Siberia, the apparent low temperature limit of natural distribution, became available, which may be a source for chilling tolerance. The collection was screened for chilling tolerance of photosynthesis by measuring dark-adapted maximum quantum yield of PSII photochemistry (Fv/Fm) on plants in the field in cool weather. Superior accessions were selected for further phenotyping: plants were grown at 25°C, transferred to 10°C (chilling) for 15 days, and returned to 25°C for 7 days (recovery). Two experiments assessed: (a) light-saturated net photosynthetic rate (Asat) and operating quantum yield of PSII photochemistry (ΦPSII), (b) response of net leaf CO2 uptake (A) to intercellular [CO2] (ci). Three accessions showed superior chilling tolerance: RU2012-069 and RU2012-114 achieved Asat up to double that of M. x giganteus prior to and during chilling, due to increased ci - saturated photosynthesis (Vmax). RU2012-069 and RU2012-114 also maintained greater levels of ΦPSII during chilling, indicating reduced photodamage. Additionally, accession RU2012-112 maintained a stable Asat throughout the 15-day chilling period, while Asat continuously declined in other accessions; this suggests RU2012-112 could outperform others in lengthy chilling periods. Plants were returned to 25°C after the chilling period; M. x giganteus showed the weakest recovery after 1 day, but a strong recovery after 1 week. This study has therefore identified important genetic resources for the synthesis of improved lines of M. x giganteus, which could facilitate the displacement of fossil fuels by cellulosic bioenergy. © 2019 The Authors. GCB Bioenergy Published by John Wiley & Sons Ltd.
AB - Chilling temperatures (0–15°C) inhibit photosynthesis in most C4 grasses, yet photosynthesis is chilling tolerant in the ‘Illinois’ clone of the C4 grass Miscanthus x giganteus, a candidate cellulosic bioenergy crop. M. x giganteus is a hybrid between Miscanthus sacchariflorus and Miscanthus sinensis; therefore chilling-tolerant parent lines might produce hybrids superior to the current clone. Recently a collection of M. sacchariflorus from Siberia, the apparent low temperature limit of natural distribution, became available, which may be a source for chilling tolerance. The collection was screened for chilling tolerance of photosynthesis by measuring dark-adapted maximum quantum yield of PSII photochemistry (Fv/Fm) on plants in the field in cool weather. Superior accessions were selected for further phenotyping: plants were grown at 25°C, transferred to 10°C (chilling) for 15 days, and returned to 25°C for 7 days (recovery). Two experiments assessed: (a) light-saturated net photosynthetic rate (Asat) and operating quantum yield of PSII photochemistry (ΦPSII), (b) response of net leaf CO2 uptake (A) to intercellular [CO2] (ci). Three accessions showed superior chilling tolerance: RU2012-069 and RU2012-114 achieved Asat up to double that of M. x giganteus prior to and during chilling, due to increased ci - saturated photosynthesis (Vmax). RU2012-069 and RU2012-114 also maintained greater levels of ΦPSII during chilling, indicating reduced photodamage. Additionally, accession RU2012-112 maintained a stable Asat throughout the 15-day chilling period, while Asat continuously declined in other accessions; this suggests RU2012-112 could outperform others in lengthy chilling periods. Plants were returned to 25°C after the chilling period; M. x giganteus showed the weakest recovery after 1 day, but a strong recovery after 1 week. This study has therefore identified important genetic resources for the synthesis of improved lines of M. x giganteus, which could facilitate the displacement of fossil fuels by cellulosic bioenergy. © 2019 The Authors. GCB Bioenergy Published by John Wiley & Sons Ltd.
KW - bioenergy
KW - biomass
KW - chilling tolerance
KW - cold tolerance
KW - Miscanthus sacchariflorus
KW - Miscanthus x giganteus
KW - photosynthesis
KW - photosystem II
KW - Siberia
KW - Biofuels
KW - Biomass
KW - Carbon dioxide
KW - Cloning
KW - Crops
KW - Fossil fuels
KW - Photosynthesis
KW - Quantum yield
KW - Recovery
KW - Temperature
KW - Bio-energy
KW - Chilling tolerance
KW - Cold tolerance
KW - Miscanthus
KW - Photosystem II
KW - SIBERIA
KW - Plants (botany)
KW - Miscanthus sinensis
KW - Poaceae
U2 - 10.1111/gcbb.12599
DO - 10.1111/gcbb.12599
M3 - Journal article
VL - 11
SP - 883
EP - 894
JO - GCB Bioenergy
JF - GCB Bioenergy
SN - 1757-1693
IS - 7
ER -