Rights statement: This is an author-created, un-copyedited version of an article accepted for publication/published in Biomedical Materials. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at doi: 10.1088/1748-605X/aa999b
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Silk fibroin/amniotic membrane 3D bi-layered artificial skin
AU - Gholipourmalekabadi, Mazaher
AU - Samadikuchaksaraei, Ali
AU - Seifalian, Alexander
AU - Urbanska, Aleksandra
AU - Ghanbarian, Hossein
AU - Hardy, John George
AU - Omrani, Mir
AU - Mozafari, Masoud
AU - Reis, Rui
AU - Kundu, Subhas
N1 - This is an author-created, un-copyedited version of an article accepted for publication/published in Biomedical Materials. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at doi: 10.1088/1748-605X/aa999b
PY - 2018/2/20
Y1 - 2018/2/20
N2 - Burn injury has been reported to be an important cause of morbidity and mortality and it is still considered as unmet clinical need. Although there is a myriad of effective stem cells suggested for skin regeneration, there is no one ideal scaffold. The aim of this study was to develop a 3D bi-layer scaffold made of biological decellularized human amniotic membrane (AM) with viscoelastic electrospun nanofibrous silk fibroin (ESF) spun on top. The fabricated 3D bi-layer AM/ESF scaffold was submerged in ethanol to induce β-sheet transformation as well as to get a tightly coated and inseparable bilayer. The biomechanical and biological properties of the 3D bi-layer AM/ESF scaffold were investigated. The results indicate a significant improved mechanical properties of the AM/ESF compared to the AM alone. Both AM and AM/ESF possess a variety of suitable adhesion cells without detectable cytotoxicity against the Adipose Tissue-Derived Mesenchymal Stem Cells (AT-MSCs). The AT-MSCs show increased expression of two main pro-angiogenesis factors VEGFa and bFGF when cultured on the AM/ESF for 7 days in comparison with AM alone. The results suggest that AM/ESF scaffold with autologous AT-MSCs has excellent cell adhesion and proliferation along with production of growth factors which serves as a possible application in clinical setting in skin regeneration.
AB - Burn injury has been reported to be an important cause of morbidity and mortality and it is still considered as unmet clinical need. Although there is a myriad of effective stem cells suggested for skin regeneration, there is no one ideal scaffold. The aim of this study was to develop a 3D bi-layer scaffold made of biological decellularized human amniotic membrane (AM) with viscoelastic electrospun nanofibrous silk fibroin (ESF) spun on top. The fabricated 3D bi-layer AM/ESF scaffold was submerged in ethanol to induce β-sheet transformation as well as to get a tightly coated and inseparable bilayer. The biomechanical and biological properties of the 3D bi-layer AM/ESF scaffold were investigated. The results indicate a significant improved mechanical properties of the AM/ESF compared to the AM alone. Both AM and AM/ESF possess a variety of suitable adhesion cells without detectable cytotoxicity against the Adipose Tissue-Derived Mesenchymal Stem Cells (AT-MSCs). The AT-MSCs show increased expression of two main pro-angiogenesis factors VEGFa and bFGF when cultured on the AM/ESF for 7 days in comparison with AM alone. The results suggest that AM/ESF scaffold with autologous AT-MSCs has excellent cell adhesion and proliferation along with production of growth factors which serves as a possible application in clinical setting in skin regeneration.
KW - silk
KW - decellularized
KW - tissue engineering
KW - chemical engineering
KW - biomedical engineering
KW - materials science
KW - wound healing
KW - biomaterials
U2 - 10.1088/1748-605X/aa999b
DO - 10.1088/1748-605X/aa999b
M3 - Journal article
VL - 13
JO - Biomedical Materials
JF - Biomedical Materials
SN - 1748-6041
IS - 3
M1 - 035003
ER -