Home > Research > Publications & Outputs > Single-cell Raman microscopy of microengineered...

Links

Text available via DOI:

View graph of relations

Single-cell Raman microscopy of microengineered cell scaffolds

Research output: Contribution to journalJournal article

E-pub ahead of print

Standard

Single-cell Raman microscopy of microengineered cell scaffolds. / Baldock, Sara Jane; Talari, Abdullah Chandra Sekhar; Smith, Rachael; Wright, Karen Leslie; Ashton, Lorna.

In: Journal of Raman Spectroscopy, 04.12.2018.

Research output: Contribution to journalJournal article

Harvard

APA

Vancouver

Author

Bibtex

@article{7ee0d0983e5b4e5994266fe6ad571525,
title = "Single-cell Raman microscopy of microengineered cell scaffolds",
abstract = "Studying cells in a three‐dimensional (3D) environment has great potential in understanding cell behaviours such as morphology, proliferation, differentiation, and migration. Microengineered 3D cell scaffolds with precise defined geometries have offered a new approach to study cell behaviour and its interactions with scaffolds. The use of Raman spectroscopy to characterise biomolecules is a rapidly expanding area and has been implemented in numerous fields including pharmacology, microbiology, toxicology, and single‐cell studies. However, one area where it remains unexploited despite the vast potential of the technique is in the investigation of 3D cell scaffolds. A combination of Raman microscopy and chemometric approaches have employed to investigate the structure and biochemistry of nanofabricated scaffolds and a cell–scaffold complex. The 3D Raman mapping combined with the use of nanofabricated 3D scaffolds offers a unique opportunity to assess the influence of scaffold architecture on cell body and cell nuclei morphology and biochemistry. For the first time, we have cultured a human epithelial colorectal adenocarcinoma cell line on OrmoComp scaffolds and determined the structure and biochemistry of nanofabricated scaffolds and a cell–scaffold complex with the use of Raman microscopy combined with appropriate data analysis protocols. The results demonstrate the potential of 3D Raman mapping for identifying biochemical and physical variation within single cells as they grow and adhere to 3D scaffolds.",
keywords = "cell culture, principle component analysis, single cell Raman microscopy, 3D cell scaffolds, 3D mapping",
author = "Baldock, {Sara Jane} and Talari, {Abdullah Chandra Sekhar} and Rachael Smith and Wright, {Karen Leslie} and Lorna Ashton",
year = "2018",
month = "12",
day = "4",
doi = "10.1002/jrs.5525",
language = "English",
journal = "Journal of Raman Spectroscopy",
issn = "0377-0486",
publisher = "John Wiley and Sons Ltd",

}

RIS

TY - JOUR

T1 - Single-cell Raman microscopy of microengineered cell scaffolds

AU - Baldock, Sara Jane

AU - Talari, Abdullah Chandra Sekhar

AU - Smith, Rachael

AU - Wright, Karen Leslie

AU - Ashton, Lorna

PY - 2018/12/4

Y1 - 2018/12/4

N2 - Studying cells in a three‐dimensional (3D) environment has great potential in understanding cell behaviours such as morphology, proliferation, differentiation, and migration. Microengineered 3D cell scaffolds with precise defined geometries have offered a new approach to study cell behaviour and its interactions with scaffolds. The use of Raman spectroscopy to characterise biomolecules is a rapidly expanding area and has been implemented in numerous fields including pharmacology, microbiology, toxicology, and single‐cell studies. However, one area where it remains unexploited despite the vast potential of the technique is in the investigation of 3D cell scaffolds. A combination of Raman microscopy and chemometric approaches have employed to investigate the structure and biochemistry of nanofabricated scaffolds and a cell–scaffold complex. The 3D Raman mapping combined with the use of nanofabricated 3D scaffolds offers a unique opportunity to assess the influence of scaffold architecture on cell body and cell nuclei morphology and biochemistry. For the first time, we have cultured a human epithelial colorectal adenocarcinoma cell line on OrmoComp scaffolds and determined the structure and biochemistry of nanofabricated scaffolds and a cell–scaffold complex with the use of Raman microscopy combined with appropriate data analysis protocols. The results demonstrate the potential of 3D Raman mapping for identifying biochemical and physical variation within single cells as they grow and adhere to 3D scaffolds.

AB - Studying cells in a three‐dimensional (3D) environment has great potential in understanding cell behaviours such as morphology, proliferation, differentiation, and migration. Microengineered 3D cell scaffolds with precise defined geometries have offered a new approach to study cell behaviour and its interactions with scaffolds. The use of Raman spectroscopy to characterise biomolecules is a rapidly expanding area and has been implemented in numerous fields including pharmacology, microbiology, toxicology, and single‐cell studies. However, one area where it remains unexploited despite the vast potential of the technique is in the investigation of 3D cell scaffolds. A combination of Raman microscopy and chemometric approaches have employed to investigate the structure and biochemistry of nanofabricated scaffolds and a cell–scaffold complex. The 3D Raman mapping combined with the use of nanofabricated 3D scaffolds offers a unique opportunity to assess the influence of scaffold architecture on cell body and cell nuclei morphology and biochemistry. For the first time, we have cultured a human epithelial colorectal adenocarcinoma cell line on OrmoComp scaffolds and determined the structure and biochemistry of nanofabricated scaffolds and a cell–scaffold complex with the use of Raman microscopy combined with appropriate data analysis protocols. The results demonstrate the potential of 3D Raman mapping for identifying biochemical and physical variation within single cells as they grow and adhere to 3D scaffolds.

KW - cell culture

KW - principle component analysis

KW - single cell Raman microscopy

KW - 3D cell scaffolds

KW - 3D mapping

U2 - 10.1002/jrs.5525

DO - 10.1002/jrs.5525

M3 - Journal article

JO - Journal of Raman Spectroscopy

JF - Journal of Raman Spectroscopy

SN - 0377-0486

ER -