Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Sister chromatid junctions in the hyperthermophilic archaeon Sulfolobus solfataricus
AU - Robinson, Nicholas P.
AU - Blood, Katherine A.
AU - McCallum, Simon A.
AU - Edwards, Paul A. W.
AU - Bell, Stephen D.
PY - 2007/2/7
Y1 - 2007/2/7
N2 - Although the Archaea exhibit an intriguing combination of bacterial- and eukaryotic-like features, it is not known how these prokaryotic cells segregate their chromosomes before the process of cell division. In the course of our analysis of the third replication origin in the archaeon Sulfolobus solfataricus, we identify and characterise sister chromatid junctions in this prokaryote. This pairing appears to be mediated by hemicatenane-like structures, and we provide evidence that these junctions persist in both replicating and postreplicative cells. These data, in conjunction with fluorescent in situ hybridisation analyses, suggest that Sulfolobus chromosomes have a significant period of postreplicative sister chromatid synapsis, a situation that is more reminiscent of eukaryotic than bacterial chromosome segregation mechanisms.
AB - Although the Archaea exhibit an intriguing combination of bacterial- and eukaryotic-like features, it is not known how these prokaryotic cells segregate their chromosomes before the process of cell division. In the course of our analysis of the third replication origin in the archaeon Sulfolobus solfataricus, we identify and characterise sister chromatid junctions in this prokaryote. This pairing appears to be mediated by hemicatenane-like structures, and we provide evidence that these junctions persist in both replicating and postreplicative cells. These data, in conjunction with fluorescent in situ hybridisation analyses, suggest that Sulfolobus chromosomes have a significant period of postreplicative sister chromatid synapsis, a situation that is more reminiscent of eukaryotic than bacterial chromosome segregation mechanisms.
KW - Chromatids
KW - DNA Primers
KW - Electrophoresis, Gel, Two-Dimensional
KW - Flow Cytometry
KW - In Situ Hybridization, Fluorescence
KW - Origin Recognition Complex
KW - Sister Chromatid Exchange
KW - Sulfolobus solfataricus
KW - Comparative Study
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1038/sj.emboj.7601529
DO - 10.1038/sj.emboj.7601529
M3 - Journal article
C2 - 17255945
VL - 26
SP - 816
EP - 824
JO - EMBO Journal
JF - EMBO Journal
SN - 0261-4189
IS - 3
ER -