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    Rights statement: This is the author’s version of a work that was accepted for publication in Journal of Molecular Structure. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Molecular Structure, 1106, 2016 DOI: 10.1016/j.molstruc.2015.11.003

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Spatial and temporal age-related spectral alterations in benign human breast tissue

Research output: Contribution to journalJournal article

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Spatial and temporal age-related spectral alterations in benign human breast tissue. / Theophilou, Georgios; Fogarty, Simon; Trevisan, Julio; Strong, Becky; Heys, Kelly; Patel, Imran; Stringfellow, Helen F.; Martin-Hirsch, Pierre Leonard; Martin, Francis Luke.

In: Journal of Molecular Structure, Vol. 1106, 15.02.2016, p. 390-398.

Research output: Contribution to journalJournal article

Harvard

Theophilou, G, Fogarty, S, Trevisan, J, Strong, B, Heys, K, Patel, I, Stringfellow, HF, Martin-Hirsch, PL & Martin, FL 2016, 'Spatial and temporal age-related spectral alterations in benign human breast tissue', Journal of Molecular Structure, vol. 1106, pp. 390-398. https://doi.org/10.1016/j.molstruc.2015.11.003

APA

Theophilou, G., Fogarty, S., Trevisan, J., Strong, B., Heys, K., Patel, I., Stringfellow, H. F., Martin-Hirsch, P. L., & Martin, F. L. (2016). Spatial and temporal age-related spectral alterations in benign human breast tissue. Journal of Molecular Structure, 1106, 390-398. https://doi.org/10.1016/j.molstruc.2015.11.003

Vancouver

Author

Theophilou, Georgios ; Fogarty, Simon ; Trevisan, Julio ; Strong, Becky ; Heys, Kelly ; Patel, Imran ; Stringfellow, Helen F. ; Martin-Hirsch, Pierre Leonard ; Martin, Francis Luke. / Spatial and temporal age-related spectral alterations in benign human breast tissue. In: Journal of Molecular Structure. 2016 ; Vol. 1106. pp. 390-398.

Bibtex

@article{58470c2f1f484769a588a3b3b53fb3ef,
title = "Spatial and temporal age-related spectral alterations in benign human breast tissue",
abstract = "Epidemiological evidence suggests that cancers attributable to exogenous carcinogenic agents may appear decades after initiating exposures. Environmental factors including lifestyle and/or diet have been implicated in the aetiology of breast cancer. Breast tissue undergoes continuous molecular and morphological changes from the time of thelarche to menopause and thereafter. These alterations are both cyclical and longitudinal, and can be influenced by several environmental factors including exposure to oestrogens. Research into the latent period leading to breast carcinogenesis has been mostly limited to when hyperplastic lesions are present. Investigations to identify a biomarker of commitment to disease in normal breast tissue are hindered by the molecular and histological diversity of disease-free breast tissue. Benign tissue from reduction mammoplasties provides an opportunity to study biochemical differences between women of similar ages as well as alterations with advancing age. Herein, synchrotron radiation-based Fourier-transform infrared (SR-FTIR) microspectroscopy was used to examine the terminal ductal lobular epithelium (TDLU) and, intra- and inter-lobular epithelium to identify spatial and temporal changes within these areas. Principal component analysis (PCA) followed by linear discriminant analysis of mid-infrared spectra revealed unambiguous inter-individual as well as age-related differences in each histological compartment interrogated. Moreover, exploratory PCA of luminal and myoepithelial cells within the TDLU indicated the presence of specific cells, potentially stem cells. Understanding alterations within benign tissue may assist in the identification of alterations in latent pre-clinical stages of breast cancer.",
keywords = "Aetiology, Biospectroscopy, Breast cancer, Multivariate analysis, Synchrotron radiation-based Fourier-transform infrared (SR-FTIR) microspectroscopy, Terminal ductal lobular unit",
author = "Georgios Theophilou and Simon Fogarty and Julio Trevisan and Becky Strong and Kelly Heys and Imran Patel and Stringfellow, {Helen F.} and Martin-Hirsch, {Pierre Leonard} and Martin, {Francis Luke}",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Journal of Molecular Structure. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Molecular Structure, 1106, 2016 DOI: 10.1016/j.molstruc.2015.11.003",
year = "2016",
month = feb,
day = "15",
doi = "10.1016/j.molstruc.2015.11.003",
language = "English",
volume = "1106",
pages = "390--398",
journal = "Journal of Molecular Structure",
issn = "0022-2860",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Spatial and temporal age-related spectral alterations in benign human breast tissue

AU - Theophilou, Georgios

AU - Fogarty, Simon

AU - Trevisan, Julio

AU - Strong, Becky

AU - Heys, Kelly

AU - Patel, Imran

AU - Stringfellow, Helen F.

AU - Martin-Hirsch, Pierre Leonard

AU - Martin, Francis Luke

N1 - This is the author’s version of a work that was accepted for publication in Journal of Molecular Structure. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Molecular Structure, 1106, 2016 DOI: 10.1016/j.molstruc.2015.11.003

PY - 2016/2/15

Y1 - 2016/2/15

N2 - Epidemiological evidence suggests that cancers attributable to exogenous carcinogenic agents may appear decades after initiating exposures. Environmental factors including lifestyle and/or diet have been implicated in the aetiology of breast cancer. Breast tissue undergoes continuous molecular and morphological changes from the time of thelarche to menopause and thereafter. These alterations are both cyclical and longitudinal, and can be influenced by several environmental factors including exposure to oestrogens. Research into the latent period leading to breast carcinogenesis has been mostly limited to when hyperplastic lesions are present. Investigations to identify a biomarker of commitment to disease in normal breast tissue are hindered by the molecular and histological diversity of disease-free breast tissue. Benign tissue from reduction mammoplasties provides an opportunity to study biochemical differences between women of similar ages as well as alterations with advancing age. Herein, synchrotron radiation-based Fourier-transform infrared (SR-FTIR) microspectroscopy was used to examine the terminal ductal lobular epithelium (TDLU) and, intra- and inter-lobular epithelium to identify spatial and temporal changes within these areas. Principal component analysis (PCA) followed by linear discriminant analysis of mid-infrared spectra revealed unambiguous inter-individual as well as age-related differences in each histological compartment interrogated. Moreover, exploratory PCA of luminal and myoepithelial cells within the TDLU indicated the presence of specific cells, potentially stem cells. Understanding alterations within benign tissue may assist in the identification of alterations in latent pre-clinical stages of breast cancer.

AB - Epidemiological evidence suggests that cancers attributable to exogenous carcinogenic agents may appear decades after initiating exposures. Environmental factors including lifestyle and/or diet have been implicated in the aetiology of breast cancer. Breast tissue undergoes continuous molecular and morphological changes from the time of thelarche to menopause and thereafter. These alterations are both cyclical and longitudinal, and can be influenced by several environmental factors including exposure to oestrogens. Research into the latent period leading to breast carcinogenesis has been mostly limited to when hyperplastic lesions are present. Investigations to identify a biomarker of commitment to disease in normal breast tissue are hindered by the molecular and histological diversity of disease-free breast tissue. Benign tissue from reduction mammoplasties provides an opportunity to study biochemical differences between women of similar ages as well as alterations with advancing age. Herein, synchrotron radiation-based Fourier-transform infrared (SR-FTIR) microspectroscopy was used to examine the terminal ductal lobular epithelium (TDLU) and, intra- and inter-lobular epithelium to identify spatial and temporal changes within these areas. Principal component analysis (PCA) followed by linear discriminant analysis of mid-infrared spectra revealed unambiguous inter-individual as well as age-related differences in each histological compartment interrogated. Moreover, exploratory PCA of luminal and myoepithelial cells within the TDLU indicated the presence of specific cells, potentially stem cells. Understanding alterations within benign tissue may assist in the identification of alterations in latent pre-clinical stages of breast cancer.

KW - Aetiology

KW - Biospectroscopy

KW - Breast cancer

KW - Multivariate analysis

KW - Synchrotron radiation-based Fourier-transform infrared (SR-FTIR) microspectroscopy

KW - Terminal ductal lobular unit

U2 - 10.1016/j.molstruc.2015.11.003

DO - 10.1016/j.molstruc.2015.11.003

M3 - Journal article

VL - 1106

SP - 390

EP - 398

JO - Journal of Molecular Structure

JF - Journal of Molecular Structure

SN - 0022-2860

ER -