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Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSNChapter (peer-reviewed)

Published

Standard

Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts. / Sales, CRG; Degen, Gustaf E.; Bernardes Da Silva, Anabela; Carmo-Silva, Ana Elizabete.

Photosynthesis: Methods and Protocols. ed. / Sarah Covshoff. Vol. 1770 Niceville, FL, USA : Springer Science + Business Media, 2018. p. 239-250.

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSNChapter (peer-reviewed)

Harvard

Sales, CRG, Degen, GE, Bernardes Da Silva, A & Carmo-Silva, AE 2018, Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts. in S Covshoff (ed.), Photosynthesis: Methods and Protocols. vol. 1770, Springer Science + Business Media, Niceville, FL, USA, pp. 239-250.

APA

Sales, CRG., Degen, G. E., Bernardes Da Silva, A., & Carmo-Silva, A. E. (2018). Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts. In S. Covshoff (Ed.), Photosynthesis: Methods and Protocols (Vol. 1770, pp. 239-250). Springer Science + Business Media.

Vancouver

Sales CRG, Degen GE, Bernardes Da Silva A, Carmo-Silva AE. Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts. In Covshoff S, editor, Photosynthesis: Methods and Protocols. Vol. 1770. Niceville, FL, USA: Springer Science + Business Media. 2018. p. 239-250

Author

Sales, CRG ; Degen, Gustaf E. ; Bernardes Da Silva, Anabela ; Carmo-Silva, Ana Elizabete. / Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts. Photosynthesis: Methods and Protocols. editor / Sarah Covshoff. Vol. 1770 Niceville, FL, USA : Springer Science + Business Media, 2018. pp. 239-250

Bibtex

@inbook{34be6e9188844d36866b8735e5b1eb42,
title = "Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts",
abstract = "Rubisco plays a central role in photosynthesis and, due to its catalytic inefficiencies,frequently limits CO2 assimilation in fully illuminated leaves at the top of unstressed crop canopies.The CO2-fixing enzyme is heavily regulated and not all the enzyme present in the leaf is active at any given moment. In this chapter,a spectrophotometric assay is described for measuring Rubisco activity and activation state in leaf extracts.Most of the assay components are available commercially and others can be produced by established protocols, making adoption of the assay achievable by most plant biochemistry laboratories.Its relative high-throughput capacity enables large-scale experiments aimed at screening germplasm for improved Rubisco function.",
author = "CRG Sales and Degen, {Gustaf E.} and {Bernardes Da Silva}, Anabela and Carmo-Silva, {Ana Elizabete}",
year = "2018",
month = jul
day = "6",
language = "English",
isbn = "9781493977857",
volume = "1770",
pages = "239--250",
editor = "Covshoff, {Sarah }",
booktitle = "Photosynthesis",
publisher = "Springer Science + Business Media",
address = "United States",

}

RIS

TY - CHAP

T1 - Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts

AU - Sales, CRG

AU - Degen, Gustaf E.

AU - Bernardes Da Silva, Anabela

AU - Carmo-Silva, Ana Elizabete

PY - 2018/7/6

Y1 - 2018/7/6

N2 - Rubisco plays a central role in photosynthesis and, due to its catalytic inefficiencies,frequently limits CO2 assimilation in fully illuminated leaves at the top of unstressed crop canopies.The CO2-fixing enzyme is heavily regulated and not all the enzyme present in the leaf is active at any given moment. In this chapter,a spectrophotometric assay is described for measuring Rubisco activity and activation state in leaf extracts.Most of the assay components are available commercially and others can be produced by established protocols, making adoption of the assay achievable by most plant biochemistry laboratories.Its relative high-throughput capacity enables large-scale experiments aimed at screening germplasm for improved Rubisco function.

AB - Rubisco plays a central role in photosynthesis and, due to its catalytic inefficiencies,frequently limits CO2 assimilation in fully illuminated leaves at the top of unstressed crop canopies.The CO2-fixing enzyme is heavily regulated and not all the enzyme present in the leaf is active at any given moment. In this chapter,a spectrophotometric assay is described for measuring Rubisco activity and activation state in leaf extracts.Most of the assay components are available commercially and others can be produced by established protocols, making adoption of the assay achievable by most plant biochemistry laboratories.Its relative high-throughput capacity enables large-scale experiments aimed at screening germplasm for improved Rubisco function.

M3 - Chapter (peer-reviewed)

SN - 9781493977857

VL - 1770

SP - 239

EP - 250

BT - Photosynthesis

A2 - Covshoff, Sarah

PB - Springer Science + Business Media

CY - Niceville, FL, USA

ER -