Spectroscopic characterisation of disaccharides derived from keratan sulfates.

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Spectroscopic characterisation of disaccharides derived from keratan sulfates. / Huckerby, Thomas N.; Brown, Gavin M.; Dickenson, John M. et al.
In: European Journal of Biochemistry, Vol. 229, No. 1, 1995, p. 119-131.

Research output: Contribution to Journal/Magazine › Journal article

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Huckerby, Thomas N. ; Brown, Gavin M. ; Dickenson, John M. et al. / Spectroscopic characterisation of disaccharides derived from keratan sulfates. In: European Journal of Biochemistry. 1995 ; Vol. 229, No. 1. pp. 119-131.

Bibtex

@article{ee83372e76874e63b211c129074f60b0,

title = "Spectroscopic characterisation of disaccharides derived from keratan sulfates.",

abstract = "Skeletal keratan sulfates have been degraded by three independent techniques and the resultant, borohydride-reduced, disaccharides have been characterised by NMR spectroscopy. The ¹H and 13C (where available) chemical shifts are reported for the following substances, where GalNAc-ol represents Nacetyl-galactosaminitol. GlcNAc-ol represents N-acetyl-glucosaminitol, GlcNAc(6S)-ol represents N-acetyl-glucosaminitol 6-O-sulfate and 2,5AnMan(6S)-ol represents 2.5-anhydro-D-mannitol 6-O-sulfate. (a) GlcNAc(6S)β(1-3)Gal-ol, isolated after keratanase (from Pseudomonas sp.) digestion. (b) Galβ(14)GlcNAc(6S)-ol and Gal(6S)β(1-4) GlcNAc(6S)-ol, the ¹H chemical shills have been reported previously [Brown, G. M., Huckerby, T. N., Morris, H. G., Abram, B. L. & Nieduszynski, I. A, (1994) Biochemistry 33, 4836-4846; Brown, G. M., Huckerby, T. N. & Nieduszynski, I. A. (1994) Eur: J. Biochem. 224, 281-308], GlcNAc(6S)β(1-6)GalNAc-ol, Galβ(14)GlcNAc-ol, Gal(6S)β(1-4)GlcNAc-ol, all isolated after keratanase II digestion, (c) Galβ(14)2,5AnMan(6S)-ol and Gal(6S)β(1-4)2.5AnMan(6S)-ol, isolated after hydrazinolysis and nitrous acid digestion. In addition, the model compounds Galβ(1-4)GlcNAc-ol and Galβ(1-6)GlcNAc-ol have also been examined by ¹H and 13C NMR spectroscopy. The value of these data for microstructural analysis of keratan sulfate samples is discussed.",

author = "Huckerby, {Thomas N.} and Brown, {Gavin M.} and Dickenson, {John M.} and Nieduszynski, {Ian A.}",

year = "1995",

language = "English",

volume = "229",

pages = "119--131",

journal = "European Journal of Biochemistry",

issn = "1432-1033",

publisher = "Wiley-Blackwell",

number = "1",

}

RIS

TY - JOUR

T1 - Spectroscopic characterisation of disaccharides derived from keratan sulfates.

AU - Huckerby, Thomas N.

AU - Brown, Gavin M.

AU - Dickenson, John M.

AU - Nieduszynski, Ian A.

PY - 1995

Y1 - 1995

N2 - Skeletal keratan sulfates have been degraded by three independent techniques and the resultant, borohydride-reduced, disaccharides have been characterised by NMR spectroscopy. The ¹H and 13C (where available) chemical shifts are reported for the following substances, where GalNAc-ol represents Nacetyl-galactosaminitol. GlcNAc-ol represents N-acetyl-glucosaminitol, GlcNAc(6S)-ol represents N-acetyl-glucosaminitol 6-O-sulfate and 2,5AnMan(6S)-ol represents 2.5-anhydro-D-mannitol 6-O-sulfate. (a) GlcNAc(6S)β(1-3)Gal-ol, isolated after keratanase (from Pseudomonas sp.) digestion. (b) Galβ(14)GlcNAc(6S)-ol and Gal(6S)β(1-4) GlcNAc(6S)-ol, the ¹H chemical shills have been reported previously [Brown, G. M., Huckerby, T. N., Morris, H. G., Abram, B. L. & Nieduszynski, I. A, (1994) Biochemistry 33, 4836-4846; Brown, G. M., Huckerby, T. N. & Nieduszynski, I. A. (1994) Eur: J. Biochem. 224, 281-308], GlcNAc(6S)β(1-6)GalNAc-ol, Galβ(14)GlcNAc-ol, Gal(6S)β(1-4)GlcNAc-ol, all isolated after keratanase II digestion, (c) Galβ(14)2,5AnMan(6S)-ol and Gal(6S)β(1-4)2.5AnMan(6S)-ol, isolated after hydrazinolysis and nitrous acid digestion. In addition, the model compounds Galβ(1-4)GlcNAc-ol and Galβ(1-6)GlcNAc-ol have also been examined by ¹H and 13C NMR spectroscopy. The value of these data for microstructural analysis of keratan sulfate samples is discussed.

AB - Skeletal keratan sulfates have been degraded by three independent techniques and the resultant, borohydride-reduced, disaccharides have been characterised by NMR spectroscopy. The ¹H and 13C (where available) chemical shifts are reported for the following substances, where GalNAc-ol represents Nacetyl-galactosaminitol. GlcNAc-ol represents N-acetyl-glucosaminitol, GlcNAc(6S)-ol represents N-acetyl-glucosaminitol 6-O-sulfate and 2,5AnMan(6S)-ol represents 2.5-anhydro-D-mannitol 6-O-sulfate. (a) GlcNAc(6S)β(1-3)Gal-ol, isolated after keratanase (from Pseudomonas sp.) digestion. (b) Galβ(14)GlcNAc(6S)-ol and Gal(6S)β(1-4) GlcNAc(6S)-ol, the ¹H chemical shills have been reported previously [Brown, G. M., Huckerby, T. N., Morris, H. G., Abram, B. L. & Nieduszynski, I. A, (1994) Biochemistry 33, 4836-4846; Brown, G. M., Huckerby, T. N. & Nieduszynski, I. A. (1994) Eur: J. Biochem. 224, 281-308], GlcNAc(6S)β(1-6)GalNAc-ol, Galβ(14)GlcNAc-ol, Gal(6S)β(1-4)GlcNAc-ol, all isolated after keratanase II digestion, (c) Galβ(14)2,5AnMan(6S)-ol and Gal(6S)β(1-4)2.5AnMan(6S)-ol, isolated after hydrazinolysis and nitrous acid digestion. In addition, the model compounds Galβ(1-4)GlcNAc-ol and Galβ(1-6)GlcNAc-ol have also been examined by ¹H and 13C NMR spectroscopy. The value of these data for microstructural analysis of keratan sulfate samples is discussed.

M3 - Journal article

VL - 229

SP - 119

EP - 131

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

SN - 1432-1033

IS - 1

ER -

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