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Spontaneous charged lipid transfer between lipid vesicles

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Spontaneous charged lipid transfer between lipid vesicles. / Richens, Joanna L.; Tyler, Arwen I.I.; Barriga, Hanna M.G. et al.
In: Scientific Reports, Vol. 7, 12606, 03.10.2017.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Richens, JL, Tyler, AII, Barriga, HMG, Bramble, JP, Law, RV, Brooks, NJ, Seddon, JM, Ces, O & O'Shea, P 2017, 'Spontaneous charged lipid transfer between lipid vesicles', Scientific Reports, vol. 7, 12606. https://doi.org/10.1038/s41598-017-12611-0

APA

Richens, J. L., Tyler, A. I. I., Barriga, H. M. G., Bramble, J. P., Law, R. V., Brooks, N. J., Seddon, J. M., Ces, O., & O'Shea, P. (2017). Spontaneous charged lipid transfer between lipid vesicles. Scientific Reports, 7, Article 12606. https://doi.org/10.1038/s41598-017-12611-0

Vancouver

Richens JL, Tyler AII, Barriga HMG, Bramble JP, Law RV, Brooks NJ et al. Spontaneous charged lipid transfer between lipid vesicles. Scientific Reports. 2017 Oct 3;7:12606. doi: 10.1038/s41598-017-12611-0

Author

Richens, Joanna L. ; Tyler, Arwen I.I. ; Barriga, Hanna M.G. et al. / Spontaneous charged lipid transfer between lipid vesicles. In: Scientific Reports. 2017 ; Vol. 7.

Bibtex

@article{df2db4391c794de78fe23cee4a7d5f41,
title = "Spontaneous charged lipid transfer between lipid vesicles",
abstract = "{\textcopyright} 2017 The Author(s). An assay to study the spontaneous charged lipid transfer between lipid vesicles is described. A donor/acceptor vesicle system is employed, where neutrally charged acceptor vesicles are fluorescently labelled with the electrostatic membrane probe Fluoresceinphosphatidylethanolamine (FPE). Upon addition of charged donor vesicles, transfer of negatively charged lipid occurs, resulting in a fluorescently detectable change in the membrane potential of the acceptor vesicles. Using this approach we have studied the transfer properties of a range of lipids, varying both the h eadgroup and the chain length. At the low vesicle concentrations chosen, the transfer follows a first-order process where lipid monomers are transferred presumably through the aqueous solution phase from donor to acceptor vesicle. The rate of transfer decreases with increasing chain length which is consistent with energy models previously reported for lipid monomer vesicle interactions. Our assay improves on existing methods allowing the study of a range of unmodified lipids, continuous monitoring of transfer and simplified experimental procedures.",
author = "Richens, {Joanna L.} and Tyler, {Arwen I.I.} and Barriga, {Hanna M.G.} and Bramble, {Jonathan P.} and Law, {Robert V.} and Brooks, {Nicholas J.} and Seddon, {John M.} and Oscar Ces and Paul O'Shea",
year = "2017",
month = oct,
day = "3",
doi = "10.1038/s41598-017-12611-0",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Spontaneous charged lipid transfer between lipid vesicles

AU - Richens, Joanna L.

AU - Tyler, Arwen I.I.

AU - Barriga, Hanna M.G.

AU - Bramble, Jonathan P.

AU - Law, Robert V.

AU - Brooks, Nicholas J.

AU - Seddon, John M.

AU - Ces, Oscar

AU - O'Shea, Paul

PY - 2017/10/3

Y1 - 2017/10/3

N2 - © 2017 The Author(s). An assay to study the spontaneous charged lipid transfer between lipid vesicles is described. A donor/acceptor vesicle system is employed, where neutrally charged acceptor vesicles are fluorescently labelled with the electrostatic membrane probe Fluoresceinphosphatidylethanolamine (FPE). Upon addition of charged donor vesicles, transfer of negatively charged lipid occurs, resulting in a fluorescently detectable change in the membrane potential of the acceptor vesicles. Using this approach we have studied the transfer properties of a range of lipids, varying both the h eadgroup and the chain length. At the low vesicle concentrations chosen, the transfer follows a first-order process where lipid monomers are transferred presumably through the aqueous solution phase from donor to acceptor vesicle. The rate of transfer decreases with increasing chain length which is consistent with energy models previously reported for lipid monomer vesicle interactions. Our assay improves on existing methods allowing the study of a range of unmodified lipids, continuous monitoring of transfer and simplified experimental procedures.

AB - © 2017 The Author(s). An assay to study the spontaneous charged lipid transfer between lipid vesicles is described. A donor/acceptor vesicle system is employed, where neutrally charged acceptor vesicles are fluorescently labelled with the electrostatic membrane probe Fluoresceinphosphatidylethanolamine (FPE). Upon addition of charged donor vesicles, transfer of negatively charged lipid occurs, resulting in a fluorescently detectable change in the membrane potential of the acceptor vesicles. Using this approach we have studied the transfer properties of a range of lipids, varying both the h eadgroup and the chain length. At the low vesicle concentrations chosen, the transfer follows a first-order process where lipid monomers are transferred presumably through the aqueous solution phase from donor to acceptor vesicle. The rate of transfer decreases with increasing chain length which is consistent with energy models previously reported for lipid monomer vesicle interactions. Our assay improves on existing methods allowing the study of a range of unmodified lipids, continuous monitoring of transfer and simplified experimental procedures.

U2 - 10.1038/s41598-017-12611-0

DO - 10.1038/s41598-017-12611-0

M3 - Journal article

C2 - 28974701

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 12606

ER -