Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Structural, mechanical, and biocompatibility analyses of a novel dental restorative nanocomposite
AU - Khan, A.S.
AU - Wong, F.S.L.
AU - McKay, I.J.
AU - Whiley, R.A.
AU - Rehman, I.U.
PY - 2013
Y1 - 2013
N2 - Structure and biocompatibility are key parameters that determine the usefulness of dental materials for clinical use. Novel polyurethane (PU) nanocomposite material was prepared by chemically binding nanohydroxyapatite (nHA) to the diisocyanate component of the PU backbone by solvent- polymerization. nHA was incorporated into PU by the stepwise addition of monomeric units of the PU. The PU/nHA composite was analyzed by 13C Nuclear magnetic resonance (structural) and X-ray diffraction (phase analysis). The tensile strength and elastic modulus was evaluated for mechanical properties. These analyses revealed linkage between the hard- and soft-segments are urethane linkage and showed high mechanical properties with increase in content of nHA. To assess biocompatibility osteoblast cells were seeded on to the material and allowed to adhere and proliferate. Osteoblast-like cell growth and proliferation was assessed by MTS assay. It was found that cells adhered and proliferated on these novel substrates. To test bacterial adhesion discs of composite with and without nHA were incubated with standardized suspensions of oral bacterium Streptococcus sanguinis strain NCTC 7863. PU composites with nHA exhibited biocompatibility with respect to mammalian cell growth and showed significantly reduced bacterial adhesion as compared to PU alone. Copyright © 2012 Wiley Periodicals, Inc.
AB - Structure and biocompatibility are key parameters that determine the usefulness of dental materials for clinical use. Novel polyurethane (PU) nanocomposite material was prepared by chemically binding nanohydroxyapatite (nHA) to the diisocyanate component of the PU backbone by solvent- polymerization. nHA was incorporated into PU by the stepwise addition of monomeric units of the PU. The PU/nHA composite was analyzed by 13C Nuclear magnetic resonance (structural) and X-ray diffraction (phase analysis). The tensile strength and elastic modulus was evaluated for mechanical properties. These analyses revealed linkage between the hard- and soft-segments are urethane linkage and showed high mechanical properties with increase in content of nHA. To assess biocompatibility osteoblast cells were seeded on to the material and allowed to adhere and proliferate. Osteoblast-like cell growth and proliferation was assessed by MTS assay. It was found that cells adhered and proliferated on these novel substrates. To test bacterial adhesion discs of composite with and without nHA were incubated with standardized suspensions of oral bacterium Streptococcus sanguinis strain NCTC 7863. PU composites with nHA exhibited biocompatibility with respect to mammalian cell growth and showed significantly reduced bacterial adhesion as compared to PU alone. Copyright © 2012 Wiley Periodicals, Inc.
KW - biocompatibility
KW - mechanical properties
KW - nanohydroxyapatite
KW - polyurethane
KW - structural analysis
KW - Bacterial adhesion
KW - Clinical use
KW - Diisocyanates
KW - High mechanical properties
KW - Key parameters
KW - Mammalian cells
KW - Monomeric units
KW - Nano-hydroxyapatite
KW - Oral bacteria
KW - Osteoblast cells
KW - Osteoblast-like cells
KW - Phase analysis
KW - Soft segments
KW - Urethane linkage
KW - Adhesion
KW - Aerobic bacteria
KW - Cell growth
KW - Dental materials
KW - Growth kinetics
KW - Mechanical properties
KW - Nanocomposites
KW - Polyurethanes
KW - Structural analysis
KW - X ray diffraction
KW - Biocompatibility
U2 - 10.1002/app.37841
DO - 10.1002/app.37841
M3 - Journal article
VL - 127
SP - 439
EP - 447
JO - Journal of Applied Polymer Science
JF - Journal of Applied Polymer Science
SN - 0021-8995
IS - 1
ER -