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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Surveying Rubisco diversity and temperature response to improve crop photosynthetic efficiency
AU - Orr, Douglas John
AU - Melao Alcantara, Andre
AU - Kapralov, Maxim V.
AU - Andralojc, P. John
AU - Carmo-Silva, Ana Elizabete
AU - Parry, Martin Afan John
PY - 2016/10/3
Y1 - 2016/10/3
N2 - The threat to global food security of stagnating yields and population growth makes increasing crop productivity a critical goal over the coming decades. One key target for improving crop productivity and yields is increasing the efficiency of photosynthesis. Central to photosynthesis is ribulose-1,5-bisphosphate carboxylase/oxygenase, Rubisco, which is a critical but often rate-limiting component. Here we present full Rubisco catalytic properties measured at three temperatures for 75 plants species representing both crops and undomesticated plants from diverse climates. Some newly characterised Rubiscos were naturally 'better' compared to crop enzymes and have the potential to improve crop photosynthetic efficiency. The temperature response of the various catalytic parameters was largely consistent across the diverse range of species, though absolute values showed significant variation in Rubisco catalysis, even between closely related species. An analysis of residue differences amongst the species characterised identified a number of candidate amino acid substitutions that will aid in advancing engineering of improved Rubisco in crop systems. This study provides new insights on the range of Rubisco catalysis and temperature response present in nature, and provides new information to include in models from leaf to canopy and ecosystem scale.
AB - The threat to global food security of stagnating yields and population growth makes increasing crop productivity a critical goal over the coming decades. One key target for improving crop productivity and yields is increasing the efficiency of photosynthesis. Central to photosynthesis is ribulose-1,5-bisphosphate carboxylase/oxygenase, Rubisco, which is a critical but often rate-limiting component. Here we present full Rubisco catalytic properties measured at three temperatures for 75 plants species representing both crops and undomesticated plants from diverse climates. Some newly characterised Rubiscos were naturally 'better' compared to crop enzymes and have the potential to improve crop photosynthetic efficiency. The temperature response of the various catalytic parameters was largely consistent across the diverse range of species, though absolute values showed significant variation in Rubisco catalysis, even between closely related species. An analysis of residue differences amongst the species characterised identified a number of candidate amino acid substitutions that will aid in advancing engineering of improved Rubisco in crop systems. This study provides new insights on the range of Rubisco catalysis and temperature response present in nature, and provides new information to include in models from leaf to canopy and ecosystem scale.
U2 - 10.1104/pp.16.00750
DO - 10.1104/pp.16.00750
M3 - Journal article
VL - 172
SP - 707
EP - 717
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 2
ER -