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Sutureless amniotic membrane transplantation for ocular surface reconstruction with a chemically defined bioadhesive

Research output: Contribution to journalJournal articlepeer-review

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  • Maho Takaoka
  • Takahiro Nakamura
  • Hajime Sugai
  • Adam James Bentley
  • Naoki Nakajima
  • Nigel J. Fullwood
  • Norihiko Yokoi
  • Suong-Hyu Hyon
  • Shigeru Kinoshita
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<mark>Journal publication date</mark>07/2008
<mark>Journal</mark>Biomaterials
Issue number19
Volume29
Number of pages9
Pages (from-to)2923-2931
Publication StatusPublished
<mark>Original language</mark>English

Abstract

The purpose of this study was to evaluate the efficiency and safety of a sutureless amniotic membrane transplantation (AMT) for ocular surface reconstruction with a chemically defined bioadhesive (CDB). The CDB was synthesized from aldehyded polysaccharides and epsilon-poly(L-lysine), two kinds of medical and food additives, as starting materials. Biocompatibility assay indicated that the CDB showed excellent biocompatibility with in vitro and in vivo ocular surface tissues and most of the CDB was histologically degraded within 4 weeks. Sutureless AMT using the CDB was safely and successfully performed onto a rabbit scleral surface. Transplanted amniotic membrane (AM) evaluated by histological, electron microscopic- and immunohistochemical examination indicated that the CDB did not affect normal differentiation of the cells or the integrity of the surrounding tissue. Thus, this newly developed CDB was found to be very useful for sutureless AMT for ocular surface reconstruction, without considering the risk of infection. It has the ability to fix AM to the ocular surface for a long time-period without additional inflammation, scarring, or damage to the surrounding tissues. (C) 2008 Elsevier Ltd. All rights reserved.