Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Sutureless amniotic membrane transplantation for ocular surface reconstruction with a chemically defined bioadhesive
AU - Takaoka, Maho
AU - Nakamura, Takahiro
AU - Sugai, Hajime
AU - Bentley, Adam James
AU - Nakajima, Naoki
AU - Fullwood, Nigel J.
AU - Yokoi, Norihiko
AU - Hyon, Suong-Hyu
AU - Kinoshita, Shigeru
PY - 2008/7
Y1 - 2008/7
N2 - The purpose of this study was to evaluate the efficiency and safety of a sutureless amniotic membrane transplantation (AMT) for ocular surface reconstruction with a chemically defined bioadhesive (CDB). The CDB was synthesized from aldehyded polysaccharides and epsilon-poly(L-lysine), two kinds of medical and food additives, as starting materials. Biocompatibility assay indicated that the CDB showed excellent biocompatibility with in vitro and in vivo ocular surface tissues and most of the CDB was histologically degraded within 4 weeks. Sutureless AMT using the CDB was safely and successfully performed onto a rabbit scleral surface. Transplanted amniotic membrane (AM) evaluated by histological, electron microscopic- and immunohistochemical examination indicated that the CDB did not affect normal differentiation of the cells or the integrity of the surrounding tissue. Thus, this newly developed CDB was found to be very useful for sutureless AMT for ocular surface reconstruction, without considering the risk of infection. It has the ability to fix AM to the ocular surface for a long time-period without additional inflammation, scarring, or damage to the surrounding tissues. (C) 2008 Elsevier Ltd. All rights reserved.
AB - The purpose of this study was to evaluate the efficiency and safety of a sutureless amniotic membrane transplantation (AMT) for ocular surface reconstruction with a chemically defined bioadhesive (CDB). The CDB was synthesized from aldehyded polysaccharides and epsilon-poly(L-lysine), two kinds of medical and food additives, as starting materials. Biocompatibility assay indicated that the CDB showed excellent biocompatibility with in vitro and in vivo ocular surface tissues and most of the CDB was histologically degraded within 4 weeks. Sutureless AMT using the CDB was safely and successfully performed onto a rabbit scleral surface. Transplanted amniotic membrane (AM) evaluated by histological, electron microscopic- and immunohistochemical examination indicated that the CDB did not affect normal differentiation of the cells or the integrity of the surrounding tissue. Thus, this newly developed CDB was found to be very useful for sutureless AMT for ocular surface reconstruction, without considering the risk of infection. It has the ability to fix AM to the ocular surface for a long time-period without additional inflammation, scarring, or damage to the surrounding tissues. (C) 2008 Elsevier Ltd. All rights reserved.
KW - ophthalmology
KW - transplantation
KW - sutureless
KW - ocular surface
KW - bioadhesive
KW - biocompatibility
KW - SEALING CORNEAL INCISIONS
KW - STEM-CELL TRANSPLANTATION
KW - PARVOVIRUS B19 INFECTION
KW - STEVENS-JOHNSON-SYNDROME
KW - LIMBAL EPITHELIAL-CELLS
KW - TISSUE ADHESIVE
KW - FIBRIN GLUE
KW - AUTOLOGOUS TRANSPLANTATION
KW - CONJUNCTIVAL AUTOGRAFTS
KW - RECURRENT PTERYGIA
U2 - 10.1016/j.biomaterials.2008.03.027
DO - 10.1016/j.biomaterials.2008.03.027
M3 - Journal article
VL - 29
SP - 2923
EP - 2931
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 19
ER -