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α-Synuclein interacts with lipoproteins in plasma

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α-Synuclein interacts with lipoproteins in plasma. / Nouri Emamzadeh, Fatemeh; Allsop, David.

In: Journal of Molecular Neuroscience , Vol. 63, No. 2, 10.2017, p. 165-172.

Research output: Contribution to journalJournal article

Harvard

Nouri Emamzadeh, F & Allsop, D 2017, 'α-Synuclein interacts with lipoproteins in plasma', Journal of Molecular Neuroscience , vol. 63, no. 2, pp. 165-172. https://doi.org/10.1007/s12031-017-0967-0

APA

Nouri Emamzadeh, F., & Allsop, D. (2017). α-Synuclein interacts with lipoproteins in plasma. Journal of Molecular Neuroscience , 63(2), 165-172. https://doi.org/10.1007/s12031-017-0967-0

Vancouver

Nouri Emamzadeh F, Allsop D. α-Synuclein interacts with lipoproteins in plasma. Journal of Molecular Neuroscience . 2017 Oct;63(2):165-172. https://doi.org/10.1007/s12031-017-0967-0

Author

Nouri Emamzadeh, Fatemeh ; Allsop, David. / α-Synuclein interacts with lipoproteins in plasma. In: Journal of Molecular Neuroscience . 2017 ; Vol. 63, No. 2. pp. 165-172.

Bibtex

@article{5486d210288b48138d689dbb7d28340b,
title = "α-Synuclein interacts with lipoproteins in plasma",
abstract = "Parkinson’s disease (PD) is an age-related neurodegenerative disorder characterized by dopaminergic neural cell death in the substantia nigra of the brain and α-synuclein (α-syn) accumulation in Lewy bodies. α-Syn can be detected in blood and is a potential biomarker for PD. It has been shown recently that α-syn can pass through the blood-brain barrier (BBB), but the mechanism is not yet understood. We hypothesized that α-syn could interact with lipoproteins, and in association with these particles, could pass through the BBB. Here, we show that apoE, apoJ, and apoA1, but not apoB, were co-immunocaptured along with α-syn from human blood plasma, suggesting that α-syn is associated with high density lipoproteins (HDL). This association was also supported by experiments involving western blotting of plasma fractions separated by gel filtration,which revealed that α-syn was found in fractions identified as HDL. Interestingly, we could also detect α-syn and ApoJ in the intermediate fraction between HDL and LDL, referred to as lipoprotein (a) (Lp(a)), which has an important role in cholesterol metabolism. Overall, the results provide best support for the hypothesis that α-syn interacts with HDL, and this has potential implications for transport of α-syn from the brain to peripheral blood, across the BBB.",
keywords = "High density lipoproteins (HDL), plasma, synuclein",
author = "{Nouri Emamzadeh}, Fatemeh and David Allsop",
year = "2017",
month = "10",
doi = "10.1007/s12031-017-0967-0",
language = "English",
volume = "63",
pages = "165--172",
journal = "Journal of Molecular Neuroscience",
issn = "0895-8696",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - α-Synuclein interacts with lipoproteins in plasma

AU - Nouri Emamzadeh, Fatemeh

AU - Allsop, David

PY - 2017/10

Y1 - 2017/10

N2 - Parkinson’s disease (PD) is an age-related neurodegenerative disorder characterized by dopaminergic neural cell death in the substantia nigra of the brain and α-synuclein (α-syn) accumulation in Lewy bodies. α-Syn can be detected in blood and is a potential biomarker for PD. It has been shown recently that α-syn can pass through the blood-brain barrier (BBB), but the mechanism is not yet understood. We hypothesized that α-syn could interact with lipoproteins, and in association with these particles, could pass through the BBB. Here, we show that apoE, apoJ, and apoA1, but not apoB, were co-immunocaptured along with α-syn from human blood plasma, suggesting that α-syn is associated with high density lipoproteins (HDL). This association was also supported by experiments involving western blotting of plasma fractions separated by gel filtration,which revealed that α-syn was found in fractions identified as HDL. Interestingly, we could also detect α-syn and ApoJ in the intermediate fraction between HDL and LDL, referred to as lipoprotein (a) (Lp(a)), which has an important role in cholesterol metabolism. Overall, the results provide best support for the hypothesis that α-syn interacts with HDL, and this has potential implications for transport of α-syn from the brain to peripheral blood, across the BBB.

AB - Parkinson’s disease (PD) is an age-related neurodegenerative disorder characterized by dopaminergic neural cell death in the substantia nigra of the brain and α-synuclein (α-syn) accumulation in Lewy bodies. α-Syn can be detected in blood and is a potential biomarker for PD. It has been shown recently that α-syn can pass through the blood-brain barrier (BBB), but the mechanism is not yet understood. We hypothesized that α-syn could interact with lipoproteins, and in association with these particles, could pass through the BBB. Here, we show that apoE, apoJ, and apoA1, but not apoB, were co-immunocaptured along with α-syn from human blood plasma, suggesting that α-syn is associated with high density lipoproteins (HDL). This association was also supported by experiments involving western blotting of plasma fractions separated by gel filtration,which revealed that α-syn was found in fractions identified as HDL. Interestingly, we could also detect α-syn and ApoJ in the intermediate fraction between HDL and LDL, referred to as lipoprotein (a) (Lp(a)), which has an important role in cholesterol metabolism. Overall, the results provide best support for the hypothesis that α-syn interacts with HDL, and this has potential implications for transport of α-syn from the brain to peripheral blood, across the BBB.

KW - High density lipoproteins (HDL)

KW - plasma

KW - synuclein

U2 - 10.1007/s12031-017-0967-0

DO - 10.1007/s12031-017-0967-0

M3 - Journal article

VL - 63

SP - 165

EP - 172

JO - Journal of Molecular Neuroscience

JF - Journal of Molecular Neuroscience

SN - 0895-8696

IS - 2

ER -