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TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis in mice

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TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis in mice. / Steel, Nicola; Faniyi, Aduragbemi; Rahman, Sayema; Swietlik, Stefanie ; Czajkowska, Beata I; Chan, Bethany; Hardgrave, Alex; Steel, Anthony; Sparwasser, Tim; Assas, Mushref; Grencis, Richard K; Travis, Mark A; Worthington, John Joseph.

In: PLoS Pathogens, Vol. 15, No. 4, e1007657, 18.04.2019.

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Steel, N, Faniyi, A, Rahman, S, Swietlik, S, Czajkowska, BI, Chan, B, Hardgrave, A, Steel, A, Sparwasser, T, Assas, M, Grencis, RK, Travis, MA & Worthington, JJ 2019, 'TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis in mice', PLoS Pathogens, vol. 15, no. 4, e1007657. https://doi.org/10.1371/journal.ppat.1007657

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Steel, Nicola ; Faniyi, Aduragbemi ; Rahman, Sayema ; Swietlik, Stefanie ; Czajkowska, Beata I ; Chan, Bethany ; Hardgrave, Alex ; Steel, Anthony ; Sparwasser, Tim ; Assas, Mushref ; Grencis, Richard K ; Travis, Mark A ; Worthington, John Joseph. / TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis in mice. In: PLoS Pathogens. 2019 ; Vol. 15, No. 4.

Bibtex

@article{f62b3fea963c4259b897ba8af6010950,
title = "TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis in mice",
abstract = "Helminths are highly prevalent metazoan parasites that infect over a billion of the world’s population. Hosts have evolved numerous mechanisms to drive the expulsion of these parasites via Th2-driven immunity, but these responses must be tightly controlled to prevent equally devastating immunopathology. However, mechanisms that regulate this balance are still unclear. Here we show that the vigorous Th2 immune response driven by the small intestinal helminth Trichinella spiralis, is associated with increased TGFβ signalling responses in CD4+ T-cells. Mechanistically, enhanced TGFβ signalling in CD4+ T-cells is dependent on dendritic cell-mediated TGFβ activation which requires expression of the integrin αvβ8. Importantly, mice lacking integrin αvβ8 on DCs had a delayed ability to expel a T. spiralis infection, indicating an important functional role for integrin αvβ8-mediated TGFβ activation in promoting parasite expulsion. In addition to maintaining regulatory T-cell responses, the CD4+ T-cell signalling of this pleiotropic cytokine induces a Th17 response which is crucial in promoting the intestinal muscle hypercontractility that drives worm expulsion. Collectively, these results provide novel insights into intestinal helminth expulsion beyond that of classical Th2 driven immunity, and highlight the importance of IL-17 in intestinal contraction which may aid therapeutics to numerous diseases of the intestine.",
author = "Nicola Steel and Aduragbemi Faniyi and Sayema Rahman and Stefanie Swietlik and Czajkowska, {Beata I} and Bethany Chan and Alex Hardgrave and Anthony Steel and Tim Sparwasser and Mushref Assas and Grencis, {Richard K} and Travis, {Mark A} and Worthington, {John Joseph}",
year = "2019",
month = "4",
day = "18",
doi = "10.1371/journal.ppat.1007657",
language = "English",
volume = "15",
journal = "PLoS Pathogens",
issn = "1553-7366",
publisher = "Public Library of Science",
number = "4",

}

RIS

TY - JOUR

T1 - TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis in mice

AU - Steel, Nicola

AU - Faniyi, Aduragbemi

AU - Rahman, Sayema

AU - Swietlik, Stefanie

AU - Czajkowska, Beata I

AU - Chan, Bethany

AU - Hardgrave, Alex

AU - Steel, Anthony

AU - Sparwasser, Tim

AU - Assas, Mushref

AU - Grencis, Richard K

AU - Travis, Mark A

AU - Worthington, John Joseph

PY - 2019/4/18

Y1 - 2019/4/18

N2 - Helminths are highly prevalent metazoan parasites that infect over a billion of the world’s population. Hosts have evolved numerous mechanisms to drive the expulsion of these parasites via Th2-driven immunity, but these responses must be tightly controlled to prevent equally devastating immunopathology. However, mechanisms that regulate this balance are still unclear. Here we show that the vigorous Th2 immune response driven by the small intestinal helminth Trichinella spiralis, is associated with increased TGFβ signalling responses in CD4+ T-cells. Mechanistically, enhanced TGFβ signalling in CD4+ T-cells is dependent on dendritic cell-mediated TGFβ activation which requires expression of the integrin αvβ8. Importantly, mice lacking integrin αvβ8 on DCs had a delayed ability to expel a T. spiralis infection, indicating an important functional role for integrin αvβ8-mediated TGFβ activation in promoting parasite expulsion. In addition to maintaining regulatory T-cell responses, the CD4+ T-cell signalling of this pleiotropic cytokine induces a Th17 response which is crucial in promoting the intestinal muscle hypercontractility that drives worm expulsion. Collectively, these results provide novel insights into intestinal helminth expulsion beyond that of classical Th2 driven immunity, and highlight the importance of IL-17 in intestinal contraction which may aid therapeutics to numerous diseases of the intestine.

AB - Helminths are highly prevalent metazoan parasites that infect over a billion of the world’s population. Hosts have evolved numerous mechanisms to drive the expulsion of these parasites via Th2-driven immunity, but these responses must be tightly controlled to prevent equally devastating immunopathology. However, mechanisms that regulate this balance are still unclear. Here we show that the vigorous Th2 immune response driven by the small intestinal helminth Trichinella spiralis, is associated with increased TGFβ signalling responses in CD4+ T-cells. Mechanistically, enhanced TGFβ signalling in CD4+ T-cells is dependent on dendritic cell-mediated TGFβ activation which requires expression of the integrin αvβ8. Importantly, mice lacking integrin αvβ8 on DCs had a delayed ability to expel a T. spiralis infection, indicating an important functional role for integrin αvβ8-mediated TGFβ activation in promoting parasite expulsion. In addition to maintaining regulatory T-cell responses, the CD4+ T-cell signalling of this pleiotropic cytokine induces a Th17 response which is crucial in promoting the intestinal muscle hypercontractility that drives worm expulsion. Collectively, these results provide novel insights into intestinal helminth expulsion beyond that of classical Th2 driven immunity, and highlight the importance of IL-17 in intestinal contraction which may aid therapeutics to numerous diseases of the intestine.

U2 - 10.1371/journal.ppat.1007657

DO - 10.1371/journal.ppat.1007657

M3 - Journal article

VL - 15

JO - PLoS Pathogens

JF - PLoS Pathogens

SN - 1553-7366

IS - 4

M1 - e1007657

ER -