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The GreenScreen genotoxicity assay : a screening validation programme.

Research output: Contribution to Journal/MagazineJournal article

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The GreenScreen genotoxicity assay : a screening validation programme. / Cahill, P. A.; Knight, A. W.; Billinton, N. et al.
In: Mutagenesis, Vol. 19, No. 2, 03.2004, p. 105-119.

Research output: Contribution to Journal/MagazineJournal article

Harvard

Cahill, PA, Knight, AW, Billinton, N, Barker, MG, Walsh, L, Keenan, PO, Williams, CV, Tweats, DJ & Walmsley, RM 2004, 'The GreenScreen genotoxicity assay : a screening validation programme.', Mutagenesis, vol. 19, no. 2, pp. 105-119. https://doi.org/10.1093/mutage/geh015

APA

Cahill, P. A., Knight, A. W., Billinton, N., Barker, M. G., Walsh, L., Keenan, P. O., Williams, C. V., Tweats, D. J., & Walmsley, R. M. (2004). The GreenScreen genotoxicity assay : a screening validation programme. Mutagenesis, 19(2), 105-119. https://doi.org/10.1093/mutage/geh015

Vancouver

Cahill PA, Knight AW, Billinton N, Barker MG, Walsh L, Keenan PO et al. The GreenScreen genotoxicity assay : a screening validation programme. Mutagenesis. 2004 Mar;19(2):105-119. doi: 10.1093/mutage/geh015

Author

Cahill, P. A. ; Knight, A. W. ; Billinton, N. et al. / The GreenScreen genotoxicity assay : a screening validation programme. In: Mutagenesis. 2004 ; Vol. 19, No. 2. pp. 105-119.

Bibtex

@article{33a5e26d0d0f4cb6a5011f644977998a,
title = "The GreenScreen genotoxicity assay : a screening validation programme.",
abstract = "A yeast (Saccharomyces cerevisiae) DNA repair reporter assay termed the GreenScreen{\textregistered} assay (GSA) is described. This is a novel, cost-effective genotoxicity screen, developed to provide a pre-regulatory screening assay for use by the pharmaceutical industry and in other applications where significant numbers of compounds need to be tested. It provides a higher throughput and a lower compound consumption than existing eukaryotic genotoxicity assays and is sensitive to a broad spectrum of mutagens and, importantly, clastogens. We describe a simple, robust assay protocol and a validation study. The end-point of the test reflects the typically eukaryotic chromosomes and DNA metabolizing enzymes of yeast. The capacity for metabolic activation (MA) in yeast is limited compared with the mammalian liver or its extracts, but the assay does detect a subset of compounds that would require MA in existing genotoxicity tests. The GSA detects a different spectrum of compounds to bacterial genotoxicity assays and thus, together with an in silico structure–activity relationship (SAR) screen, and possibly a high throughput bacterial screen, would provide an effective preview of the regulatory battery of genotoxicity tests.",
author = "Cahill, {P. A.} and Knight, {A. W.} and N. Billinton and Barker, {M. G.} and L. Walsh and Keenan, {P. O.} and Williams, {C. V.} and Tweats, {D. J.} and Walmsley, {R. M.}",
year = "2004",
month = mar,
doi = "10.1093/mutage/geh015",
language = "English",
volume = "19",
pages = "105--119",
journal = "Mutagenesis",
issn = "1464-3804",
publisher = "OXFORD UNIV PRESS",
number = "2",

}

RIS

TY - JOUR

T1 - The GreenScreen genotoxicity assay : a screening validation programme.

AU - Cahill, P. A.

AU - Knight, A. W.

AU - Billinton, N.

AU - Barker, M. G.

AU - Walsh, L.

AU - Keenan, P. O.

AU - Williams, C. V.

AU - Tweats, D. J.

AU - Walmsley, R. M.

PY - 2004/3

Y1 - 2004/3

N2 - A yeast (Saccharomyces cerevisiae) DNA repair reporter assay termed the GreenScreen® assay (GSA) is described. This is a novel, cost-effective genotoxicity screen, developed to provide a pre-regulatory screening assay for use by the pharmaceutical industry and in other applications where significant numbers of compounds need to be tested. It provides a higher throughput and a lower compound consumption than existing eukaryotic genotoxicity assays and is sensitive to a broad spectrum of mutagens and, importantly, clastogens. We describe a simple, robust assay protocol and a validation study. The end-point of the test reflects the typically eukaryotic chromosomes and DNA metabolizing enzymes of yeast. The capacity for metabolic activation (MA) in yeast is limited compared with the mammalian liver or its extracts, but the assay does detect a subset of compounds that would require MA in existing genotoxicity tests. The GSA detects a different spectrum of compounds to bacterial genotoxicity assays and thus, together with an in silico structure–activity relationship (SAR) screen, and possibly a high throughput bacterial screen, would provide an effective preview of the regulatory battery of genotoxicity tests.

AB - A yeast (Saccharomyces cerevisiae) DNA repair reporter assay termed the GreenScreen® assay (GSA) is described. This is a novel, cost-effective genotoxicity screen, developed to provide a pre-regulatory screening assay for use by the pharmaceutical industry and in other applications where significant numbers of compounds need to be tested. It provides a higher throughput and a lower compound consumption than existing eukaryotic genotoxicity assays and is sensitive to a broad spectrum of mutagens and, importantly, clastogens. We describe a simple, robust assay protocol and a validation study. The end-point of the test reflects the typically eukaryotic chromosomes and DNA metabolizing enzymes of yeast. The capacity for metabolic activation (MA) in yeast is limited compared with the mammalian liver or its extracts, but the assay does detect a subset of compounds that would require MA in existing genotoxicity tests. The GSA detects a different spectrum of compounds to bacterial genotoxicity assays and thus, together with an in silico structure–activity relationship (SAR) screen, and possibly a high throughput bacterial screen, would provide an effective preview of the regulatory battery of genotoxicity tests.

U2 - 10.1093/mutage/geh015

DO - 10.1093/mutage/geh015

M3 - Journal article

VL - 19

SP - 105

EP - 119

JO - Mutagenesis

JF - Mutagenesis

SN - 1464-3804

IS - 2

ER -