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The influence of nitorgen supply on antioxidant enzymes in plant roots.

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The influence of nitorgen supply on antioxidant enzymes in plant roots. / Medici, Leonardo Oliveira; Azevedo, Ricardo Antunes; Smith, Richard John et al.
In: Functional Plant Biology, Vol. 31, No. 1, 2004, p. 1-9.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Medici, LO, Azevedo, RA, Smith, RJ & Lea, PJ 2004, 'The influence of nitorgen supply on antioxidant enzymes in plant roots.', Functional Plant Biology, vol. 31, no. 1, pp. 1-9. https://doi.org/10.1071/FP03130

APA

Medici, L. O., Azevedo, R. A., Smith, R. J., & Lea, P. J. (2004). The influence of nitorgen supply on antioxidant enzymes in plant roots. Functional Plant Biology, 31(1), 1-9. https://doi.org/10.1071/FP03130

Vancouver

Medici LO, Azevedo RA, Smith RJ, Lea PJ. The influence of nitorgen supply on antioxidant enzymes in plant roots. Functional Plant Biology. 2004;31(1):1-9. doi: 10.1071/FP03130

Author

Medici, Leonardo Oliveira ; Azevedo, Ricardo Antunes ; Smith, Richard John et al. / The influence of nitorgen supply on antioxidant enzymes in plant roots. In: Functional Plant Biology. 2004 ; Vol. 31, No. 1. pp. 1-9.

Bibtex

@article{e9ee902ce594422ca41e0e657f0f9722,
title = "The influence of nitorgen supply on antioxidant enzymes in plant roots.",
abstract = "Plants of Zea mays L., Hordeum vulgare L. and Arabidopsis thaliana (L.) Heynh. were grown at different concentrations of nitrogen, as NH4NO3, and the antioxidant enzyme activities and quantities in the roots, were studied. Maize plants were grown at 1 and 10 mM N for 32 d after germination and their roots were analysed by native PAGE for the isoenzymic profiles of glutathione reductase (GR; EC 1.6.4.2), catalase (CAT; EC 1.11.1.6) and superoxide dismutase (SOD; EC 1.15.1.1) and by spectrophotometric assays for total activity of CAT and GR. Barley and A. thaliana plants were grown at five N concentrations (0.25, 0.5, 1, 10 and 20 mM) for 27 and 26 d, respectively, and the isoenzymic profile of the three enzymes were analysed in their roots. The number of GR isoforms in the three plant species was increased at high N supply compared with low N. In addition, the CAT and GR activities detected in the roots were increased in plants grown at higher N. The increase in activity and number of bands of GR at high N in the roots of all three plants studied was not accompanied by a change in activity or the number of SOD isoforms. The possibility that the application of high N promotes the formation of reactive oxygen species by stimulating an increased rate of growth is discussed.",
keywords = "Arabidopsis thaliana, barley, catalase, glutathione reductase, maize, nitrogen, roots, superoxide dismutase.",
author = "Medici, {Leonardo Oliveira} and Azevedo, {Ricardo Antunes} and Smith, {Richard John} and Lea, {Peter John}",
year = "2004",
doi = "10.1071/FP03130",
language = "English",
volume = "31",
pages = "1--9",
journal = "Functional Plant Biology",
issn = "1445-4408",
publisher = "CSIRO PUBLISHING",
number = "1",

}

RIS

TY - JOUR

T1 - The influence of nitorgen supply on antioxidant enzymes in plant roots.

AU - Medici, Leonardo Oliveira

AU - Azevedo, Ricardo Antunes

AU - Smith, Richard John

AU - Lea, Peter John

PY - 2004

Y1 - 2004

N2 - Plants of Zea mays L., Hordeum vulgare L. and Arabidopsis thaliana (L.) Heynh. were grown at different concentrations of nitrogen, as NH4NO3, and the antioxidant enzyme activities and quantities in the roots, were studied. Maize plants were grown at 1 and 10 mM N for 32 d after germination and their roots were analysed by native PAGE for the isoenzymic profiles of glutathione reductase (GR; EC 1.6.4.2), catalase (CAT; EC 1.11.1.6) and superoxide dismutase (SOD; EC 1.15.1.1) and by spectrophotometric assays for total activity of CAT and GR. Barley and A. thaliana plants were grown at five N concentrations (0.25, 0.5, 1, 10 and 20 mM) for 27 and 26 d, respectively, and the isoenzymic profile of the three enzymes were analysed in their roots. The number of GR isoforms in the three plant species was increased at high N supply compared with low N. In addition, the CAT and GR activities detected in the roots were increased in plants grown at higher N. The increase in activity and number of bands of GR at high N in the roots of all three plants studied was not accompanied by a change in activity or the number of SOD isoforms. The possibility that the application of high N promotes the formation of reactive oxygen species by stimulating an increased rate of growth is discussed.

AB - Plants of Zea mays L., Hordeum vulgare L. and Arabidopsis thaliana (L.) Heynh. were grown at different concentrations of nitrogen, as NH4NO3, and the antioxidant enzyme activities and quantities in the roots, were studied. Maize plants were grown at 1 and 10 mM N for 32 d after germination and their roots were analysed by native PAGE for the isoenzymic profiles of glutathione reductase (GR; EC 1.6.4.2), catalase (CAT; EC 1.11.1.6) and superoxide dismutase (SOD; EC 1.15.1.1) and by spectrophotometric assays for total activity of CAT and GR. Barley and A. thaliana plants were grown at five N concentrations (0.25, 0.5, 1, 10 and 20 mM) for 27 and 26 d, respectively, and the isoenzymic profile of the three enzymes were analysed in their roots. The number of GR isoforms in the three plant species was increased at high N supply compared with low N. In addition, the CAT and GR activities detected in the roots were increased in plants grown at higher N. The increase in activity and number of bands of GR at high N in the roots of all three plants studied was not accompanied by a change in activity or the number of SOD isoforms. The possibility that the application of high N promotes the formation of reactive oxygen species by stimulating an increased rate of growth is discussed.

KW - Arabidopsis thaliana

KW - barley

KW - catalase

KW - glutathione reductase

KW - maize

KW - nitrogen

KW - roots

KW - superoxide dismutase.

U2 - 10.1071/FP03130

DO - 10.1071/FP03130

M3 - Journal article

VL - 31

SP - 1

EP - 9

JO - Functional Plant Biology

JF - Functional Plant Biology

SN - 1445-4408

IS - 1

ER -