Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - The respiration of protoplasts from leaves of barley infected by powdery mildew (Erysiphe graminis f.sp. hordei).
AU - McAinsh, Martin R.
AU - Ayres, Peter G.
AU - Hetherington, Alistair M.
PY - 1989
Y1 - 1989
N2 - Infection of barley leaves by powdery mildew (Erysiphe graminis f.sp. hordei) causes increased dark respiration, par tof which is associated with active host responses to infection, and a consequence of which is reduced plant growth. The pathogen cannot be grown separately from the host. Therefore, in order to examine those changes in respiratory activity peculiar to the host, attempts were made to isolate protoplasts from infected tissues, and from healthy controls. Isolation of useful numbers (> 106cm−6) of viable mesophyll protoplasts from infected tissues was possible with one among several batches of commercial ‘Cellulysin’ tested; on analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), this batch contained a low molecular weight protein at 20.1 kDa not found in other batches. In all isolated protoplasts, total respiration increased with the age of the source-leaf, but within 24 h of inoculation respiration was stimulated by infection. Protoplasts from infected leaves showed a small initial increase in the cyanide (CN)-sensitive component (cytochrome pathway) of total respiration, but an increase in the CN-insensitive component (alternative pathway) was the major cause of the stimulation of total respiration.
AB - Infection of barley leaves by powdery mildew (Erysiphe graminis f.sp. hordei) causes increased dark respiration, par tof which is associated with active host responses to infection, and a consequence of which is reduced plant growth. The pathogen cannot be grown separately from the host. Therefore, in order to examine those changes in respiratory activity peculiar to the host, attempts were made to isolate protoplasts from infected tissues, and from healthy controls. Isolation of useful numbers (> 106cm−6) of viable mesophyll protoplasts from infected tissues was possible with one among several batches of commercial ‘Cellulysin’ tested; on analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), this batch contained a low molecular weight protein at 20.1 kDa not found in other batches. In all isolated protoplasts, total respiration increased with the age of the source-leaf, but within 24 h of inoculation respiration was stimulated by infection. Protoplasts from infected leaves showed a small initial increase in the cyanide (CN)-sensitive component (cytochrome pathway) of total respiration, but an increase in the CN-insensitive component (alternative pathway) was the major cause of the stimulation of total respiration.
KW - barley
KW - mesophyll protoplasts
KW - powdery mildew (Erysiphe graminis hordei)
KW - dark respiration
KW - alternative pathway
U2 - 10.1016/0168-9452(89)90027-7
DO - 10.1016/0168-9452(89)90027-7
M3 - Journal article
VL - 64
SP - 221
EP - 230
JO - Plant Science
JF - Plant Science
SN - 0168-9452
IS - 2
ER -