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The role of dermatopontin in the stromal organisation of the cornea.

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The role of dermatopontin in the stromal organisation of the cornea. / Cooper, Leanne J.; Bentley, Adam J.; Nieduszynski, Ian A.; Talabani, Sheelan; Thomson, Alan; Utani, Atsushi; Shinkai, Hiroshi; Fullwood, Nigel J.; Brown, Gavin M.

In: Investigative Ophthalmology and Visual Science, Vol. 47, No. 8, 08.2006, p. 3303-3310.

Research output: Contribution to journalJournal article

Harvard

Cooper, LJ, Bentley, AJ, Nieduszynski, IA, Talabani, S, Thomson, A, Utani, A, Shinkai, H, Fullwood, NJ & Brown, GM 2006, 'The role of dermatopontin in the stromal organisation of the cornea.', Investigative Ophthalmology and Visual Science, vol. 47, no. 8, pp. 3303-3310.

APA

Cooper, L. J., Bentley, A. J., Nieduszynski, I. A., Talabani, S., Thomson, A., Utani, A., Shinkai, H., Fullwood, N. J., & Brown, G. M. (2006). The role of dermatopontin in the stromal organisation of the cornea. Investigative Ophthalmology and Visual Science, 47(8), 3303-3310.

Vancouver

Cooper LJ, Bentley AJ, Nieduszynski IA, Talabani S, Thomson A, Utani A et al. The role of dermatopontin in the stromal organisation of the cornea. Investigative Ophthalmology and Visual Science. 2006 Aug;47(8):3303-3310.

Author

Cooper, Leanne J. ; Bentley, Adam J. ; Nieduszynski, Ian A. ; Talabani, Sheelan ; Thomson, Alan ; Utani, Atsushi ; Shinkai, Hiroshi ; Fullwood, Nigel J. ; Brown, Gavin M. / The role of dermatopontin in the stromal organisation of the cornea. In: Investigative Ophthalmology and Visual Science. 2006 ; Vol. 47, No. 8. pp. 3303-3310.

Bibtex

@article{c9ffd71202994c0fb0ddacaf86222009,
title = "The role of dermatopontin in the stromal organisation of the cornea.",
abstract = "PURPOSE. Dermatopontin (DPT) is an abundant component of the stromal extracellular matrix; however, its function in the cornea is poorly understood. This study was conducted to determine whether DPT has a direct role in corneal matrix organization by investigating the ultrastructure of Dpt-null (Dpt–/–) mouse corneas. METHODS. Conventional light microscopy was used to compare the corneal thickness of Dpt–/– mice with that of the wild type. Collagen fibril distribution was studied using transmission electron microscopy and the datasets analyzed using image analysis software to determine fibrillar volume, fibril diameter, and spacing. RESULTS. Light microscopy demonstrated that Dpt–/– corneas in 2-month-old mice showed a 24% reduction in average stromal thickness compared with wild type (P < 0.001). The epithelium and Descemet{\textquoteright}s membrane appeared normal. Examination of Dpt–/– stroma by transmission electron microscopy indicated significant disruption of fibril spacing within the posterior lamellae, whereas the mid and anterior regions appeared largely unaffected compared with wild type. The collagen fibrils in Dpt–/– stroma showed a lower fibril volume fraction and a pronounced change in posterior fibrillar organization. There was no apparent difference in fibril diameter between Dpt–/– and wild-type mice. CONCLUSIONS. Collectively, these data suggest that DPT plays a key role in collagen fibril organization. The defects in collagen organization in Dpt–/– cornea appear to be most severe in the posterior stroma. It is possible that DPT interacts with corneal proteoglycans and that this interaction is involved in the maintenance of stromal architecture.",
author = "Cooper, {Leanne J.} and Bentley, {Adam J.} and Nieduszynski, {Ian A.} and Sheelan Talabani and Alan Thomson and Atsushi Utani and Hiroshi Shinkai and Fullwood, {Nigel J.} and Brown, {Gavin M.}",
year = "2006",
month = aug
language = "English",
volume = "47",
pages = "3303--3310",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "ASSOC RESEARCH VISION OPHTHALMOLOGY INC",
number = "8",

}

RIS

TY - JOUR

T1 - The role of dermatopontin in the stromal organisation of the cornea.

AU - Cooper, Leanne J.

AU - Bentley, Adam J.

AU - Nieduszynski, Ian A.

AU - Talabani, Sheelan

AU - Thomson, Alan

AU - Utani, Atsushi

AU - Shinkai, Hiroshi

AU - Fullwood, Nigel J.

AU - Brown, Gavin M.

PY - 2006/8

Y1 - 2006/8

N2 - PURPOSE. Dermatopontin (DPT) is an abundant component of the stromal extracellular matrix; however, its function in the cornea is poorly understood. This study was conducted to determine whether DPT has a direct role in corneal matrix organization by investigating the ultrastructure of Dpt-null (Dpt–/–) mouse corneas. METHODS. Conventional light microscopy was used to compare the corneal thickness of Dpt–/– mice with that of the wild type. Collagen fibril distribution was studied using transmission electron microscopy and the datasets analyzed using image analysis software to determine fibrillar volume, fibril diameter, and spacing. RESULTS. Light microscopy demonstrated that Dpt–/– corneas in 2-month-old mice showed a 24% reduction in average stromal thickness compared with wild type (P < 0.001). The epithelium and Descemet’s membrane appeared normal. Examination of Dpt–/– stroma by transmission electron microscopy indicated significant disruption of fibril spacing within the posterior lamellae, whereas the mid and anterior regions appeared largely unaffected compared with wild type. The collagen fibrils in Dpt–/– stroma showed a lower fibril volume fraction and a pronounced change in posterior fibrillar organization. There was no apparent difference in fibril diameter between Dpt–/– and wild-type mice. CONCLUSIONS. Collectively, these data suggest that DPT plays a key role in collagen fibril organization. The defects in collagen organization in Dpt–/– cornea appear to be most severe in the posterior stroma. It is possible that DPT interacts with corneal proteoglycans and that this interaction is involved in the maintenance of stromal architecture.

AB - PURPOSE. Dermatopontin (DPT) is an abundant component of the stromal extracellular matrix; however, its function in the cornea is poorly understood. This study was conducted to determine whether DPT has a direct role in corneal matrix organization by investigating the ultrastructure of Dpt-null (Dpt–/–) mouse corneas. METHODS. Conventional light microscopy was used to compare the corneal thickness of Dpt–/– mice with that of the wild type. Collagen fibril distribution was studied using transmission electron microscopy and the datasets analyzed using image analysis software to determine fibrillar volume, fibril diameter, and spacing. RESULTS. Light microscopy demonstrated that Dpt–/– corneas in 2-month-old mice showed a 24% reduction in average stromal thickness compared with wild type (P < 0.001). The epithelium and Descemet’s membrane appeared normal. Examination of Dpt–/– stroma by transmission electron microscopy indicated significant disruption of fibril spacing within the posterior lamellae, whereas the mid and anterior regions appeared largely unaffected compared with wild type. The collagen fibrils in Dpt–/– stroma showed a lower fibril volume fraction and a pronounced change in posterior fibrillar organization. There was no apparent difference in fibril diameter between Dpt–/– and wild-type mice. CONCLUSIONS. Collectively, these data suggest that DPT plays a key role in collagen fibril organization. The defects in collagen organization in Dpt–/– cornea appear to be most severe in the posterior stroma. It is possible that DPT interacts with corneal proteoglycans and that this interaction is involved in the maintenance of stromal architecture.

M3 - Journal article

VL - 47

SP - 3303

EP - 3310

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 8

ER -