Home > Research > Publications & Outputs > THE STRUCTURE OF THE KERATAN SULFATE CHAINS ATT...
View graph of relations

THE STRUCTURE OF THE KERATAN SULFATE CHAINS ATTACHED TO FIBROMODULIN ISOLATED FROM BOVINE TRACHEAL CARTILAGE - OLIGOSACCHARIDES GENERATED BY KERATANASE-II DIGESTION

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

THE STRUCTURE OF THE KERATAN SULFATE CHAINS ATTACHED TO FIBROMODULIN ISOLATED FROM BOVINE TRACHEAL CARTILAGE - OLIGOSACCHARIDES GENERATED BY KERATANASE-II DIGESTION. / LAUDER, R M ; HUCKERBY, T N ; NIEDUSZYNSKI, I A .
In: Glycoconjugate Journal, Vol. 12, No. 5, 10.1995, p. 651-659.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

APA

Vancouver

Author

Bibtex

@article{067b8d2fa2e94902933a0f95014bef99,
title = "THE STRUCTURE OF THE KERATAN SULFATE CHAINS ATTACHED TO FIBROMODULIN ISOLATED FROM BOVINE TRACHEAL CARTILAGE - OLIGOSACCHARIDES GENERATED BY KERATANASE-II DIGESTION",
abstract = "The repeat region and chain caps of the N-linked keratan sulphates attached to bovine tracheal cartilage fibromodulin were fragmented by digestion with keratanase II, and the oligosaccharides generated were isolated by strong anion-exchange chromatography. Each of these oligosaccharides has been examined by both HPAE chromatography and high field H-1-NMR spectroscopy.All of the capping oligosaccharides isolated terminated with alpha(2-3)-linked N-acetyl-neuraminic acid, and neither alpha(2-6)-linked N-acetyl-neuraminic acid chain terminators, nor fucose alpha(1-3)-linked to N-acetylglucosamine were found. The keratan sulphate chains were short, with average lengths of five to seven disaccharides, and the level of galactose sulphation varied along the length of the chain.The repeat region and chain cap were confirmed as having the following general structure:[GRAPHICS]This study has identified a novel structure in fibromodulin, namely a cap containing a sulphated galactose adjacent to a non-reducing terminal N-acetyl-neuraminic acid. We have also confirmed that the general structure of the repeat units and chain caps of N-linked keratan sulphate attached to fibromodulin isolated from bovine tracheal cartilage, is similar to that of O-linked keratan sulphate chains attached to aggrecan from non-articular cartilage. However, there are important differences in chain lengths and sulphation patterns.",
keywords = "FIBROMODULIN, SMALL PROTEOGLYCAN, KERATAN SULFATE, GLYCOSAMINOGLYCAN, SULFATION, KERATANASE II, ARTICULAR-CARTILAGE, SULFATE CHAINS, PROTEOGLYCANS, PROTEIN, ACID",
author = "LAUDER, {R M} and HUCKERBY, {T N} and NIEDUSZYNSKI, {I A}",
year = "1995",
month = oct,
doi = "10.1007/BF00731261",
language = "English",
volume = "12",
pages = "651--659",
journal = "Glycoconjugate Journal",
issn = "0282-0080",
publisher = "Springer Netherlands",
number = "5",

}

RIS

TY - JOUR

T1 - THE STRUCTURE OF THE KERATAN SULFATE CHAINS ATTACHED TO FIBROMODULIN ISOLATED FROM BOVINE TRACHEAL CARTILAGE - OLIGOSACCHARIDES GENERATED BY KERATANASE-II DIGESTION

AU - LAUDER, R M

AU - HUCKERBY, T N

AU - NIEDUSZYNSKI, I A

PY - 1995/10

Y1 - 1995/10

N2 - The repeat region and chain caps of the N-linked keratan sulphates attached to bovine tracheal cartilage fibromodulin were fragmented by digestion with keratanase II, and the oligosaccharides generated were isolated by strong anion-exchange chromatography. Each of these oligosaccharides has been examined by both HPAE chromatography and high field H-1-NMR spectroscopy.All of the capping oligosaccharides isolated terminated with alpha(2-3)-linked N-acetyl-neuraminic acid, and neither alpha(2-6)-linked N-acetyl-neuraminic acid chain terminators, nor fucose alpha(1-3)-linked to N-acetylglucosamine were found. The keratan sulphate chains were short, with average lengths of five to seven disaccharides, and the level of galactose sulphation varied along the length of the chain.The repeat region and chain cap were confirmed as having the following general structure:[GRAPHICS]This study has identified a novel structure in fibromodulin, namely a cap containing a sulphated galactose adjacent to a non-reducing terminal N-acetyl-neuraminic acid. We have also confirmed that the general structure of the repeat units and chain caps of N-linked keratan sulphate attached to fibromodulin isolated from bovine tracheal cartilage, is similar to that of O-linked keratan sulphate chains attached to aggrecan from non-articular cartilage. However, there are important differences in chain lengths and sulphation patterns.

AB - The repeat region and chain caps of the N-linked keratan sulphates attached to bovine tracheal cartilage fibromodulin were fragmented by digestion with keratanase II, and the oligosaccharides generated were isolated by strong anion-exchange chromatography. Each of these oligosaccharides has been examined by both HPAE chromatography and high field H-1-NMR spectroscopy.All of the capping oligosaccharides isolated terminated with alpha(2-3)-linked N-acetyl-neuraminic acid, and neither alpha(2-6)-linked N-acetyl-neuraminic acid chain terminators, nor fucose alpha(1-3)-linked to N-acetylglucosamine were found. The keratan sulphate chains were short, with average lengths of five to seven disaccharides, and the level of galactose sulphation varied along the length of the chain.The repeat region and chain cap were confirmed as having the following general structure:[GRAPHICS]This study has identified a novel structure in fibromodulin, namely a cap containing a sulphated galactose adjacent to a non-reducing terminal N-acetyl-neuraminic acid. We have also confirmed that the general structure of the repeat units and chain caps of N-linked keratan sulphate attached to fibromodulin isolated from bovine tracheal cartilage, is similar to that of O-linked keratan sulphate chains attached to aggrecan from non-articular cartilage. However, there are important differences in chain lengths and sulphation patterns.

KW - FIBROMODULIN

KW - SMALL PROTEOGLYCAN

KW - KERATAN SULFATE

KW - GLYCOSAMINOGLYCAN

KW - SULFATION

KW - KERATANASE II

KW - ARTICULAR-CARTILAGE

KW - SULFATE CHAINS

KW - PROTEOGLYCANS

KW - PROTEIN

KW - ACID

U2 - 10.1007/BF00731261

DO - 10.1007/BF00731261

M3 - Journal article

VL - 12

SP - 651

EP - 659

JO - Glycoconjugate Journal

JF - Glycoconjugate Journal

SN - 0282-0080

IS - 5

ER -