Home > Research > Publications & Outputs > The use of PFGE and fglagellin gene typing in i...
View graph of relations

The use of PFGE and fglagellin gene typing in identifying clonal groups of Campylobacter jejuni in farm and clinical environments.

Research output: Contribution to journalJournal article

  • Collette Fitzgerald
  • Karen Stanley
  • Sarah Andrew
  • Keith Jones
<mark>Journal publication date</mark>04/2001
<mark>Journal</mark>Applied and Environmental Microbiology
Issue number4
Number of pages8
Pages (from-to)1429-1436
Publication statusPublished
Original languageEnglish


Although campylobacters have been isolated from a wide range of animal hosts, the association between campylobacters isolated from humans and animals in the farm environment is unclear. We used flagellin gene typing and pulsed-field gel electrophoresis (PFGE) to investigate the genetic diversity among isolates from animals (cattle, sheep, and turkey) in farm environments and sporadic cases of campylobacteriosis in the same geographical area. Forty-eight combined fla types were seen among the 315 Campylobacter isolates studied. Six were found in isolates from all four hosts and represented 50% of the total number of isolates. Seventy-one different SmaI PFGE macrorestriction profiles (mrps) were observed, with 86% of isolates assigned to one of 29 different mrps. Fifty-seven isolates from diverse hosts, times, and sources had an identical SmaI mrp and combined fla type. Conversely, a number of genotypes were unique to a particular host. We provide molecular evidence which suggests a link between campylobacters in the farm environment with those causing disease in the community.