The use of PFGE and fglagellin gene typing in identifying clonal groups of Campylobacter jejuni in farm and clinical environments.

Lancaster Environment Centre

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The use of PFGE and fglagellin gene typing in identifying clonal groups of Campylobacter jejuni in farm and clinical environments. / Fitzgerald, Collette; Stanley, Karen; Andrew, Sarah et al.
In: Applied and Environmental Microbiology, Vol. 67, No. 4, 04.2001, p. 1429-1436.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Author

Fitzgerald, Collette ; Stanley, Karen ; Andrew, Sarah et al. / The use of PFGE and fglagellin gene typing in identifying clonal groups of Campylobacter jejuni in farm and clinical environments. In: Applied and Environmental Microbiology. 2001 ; Vol. 67, No. 4. pp. 1429-1436.

Bibtex

@article{9268489bf70d4dfaba0a7eefafc7f2ec,

title = "The use of PFGE and fglagellin gene typing in identifying clonal groups of Campylobacter jejuni in farm and clinical environments.",

abstract = "Although campylobacters have been isolated from a wide range of animal hosts, the association between campylobacters isolated from humans and animals in the farm environment is unclear. We used flagellin gene typing and pulsed-field gel electrophoresis (PFGE) to investigate the genetic diversity among isolates from animals (cattle, sheep, and turkey) in farm environments and sporadic cases of campylobacteriosis in the same geographical area. Forty-eight combined fla types were seen among the 315 Campylobacter isolates studied. Six were found in isolates from all four hosts and represented 50% of the total number of isolates. Seventy-one different SmaI PFGE macrorestriction profiles (mrps) were observed, with 86% of isolates assigned to one of 29 different mrps. Fifty-seven isolates from diverse hosts, times, and sources had an identical SmaI mrp and combined fla type. Conversely, a number of genotypes were unique to a particular host. We provide molecular evidence which suggests a link between campylobacters in the farm environment with those causing disease in the community.",

author = "Collette Fitzgerald and Karen Stanley and Sarah Andrew and Keith Jones",

year = "2001",

month = apr,

language = "English",

volume = "67",

pages = "1429--1436",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "4",

}

RIS

TY - JOUR

T1 - The use of PFGE and fglagellin gene typing in identifying clonal groups of Campylobacter jejuni in farm and clinical environments.

AU - Fitzgerald, Collette

AU - Stanley, Karen

AU - Andrew, Sarah

AU - Jones, Keith

PY - 2001/4

Y1 - 2001/4

N2 - Although campylobacters have been isolated from a wide range of animal hosts, the association between campylobacters isolated from humans and animals in the farm environment is unclear. We used flagellin gene typing and pulsed-field gel electrophoresis (PFGE) to investigate the genetic diversity among isolates from animals (cattle, sheep, and turkey) in farm environments and sporadic cases of campylobacteriosis in the same geographical area. Forty-eight combined fla types were seen among the 315 Campylobacter isolates studied. Six were found in isolates from all four hosts and represented 50% of the total number of isolates. Seventy-one different SmaI PFGE macrorestriction profiles (mrps) were observed, with 86% of isolates assigned to one of 29 different mrps. Fifty-seven isolates from diverse hosts, times, and sources had an identical SmaI mrp and combined fla type. Conversely, a number of genotypes were unique to a particular host. We provide molecular evidence which suggests a link between campylobacters in the farm environment with those causing disease in the community.

AB - Although campylobacters have been isolated from a wide range of animal hosts, the association between campylobacters isolated from humans and animals in the farm environment is unclear. We used flagellin gene typing and pulsed-field gel electrophoresis (PFGE) to investigate the genetic diversity among isolates from animals (cattle, sheep, and turkey) in farm environments and sporadic cases of campylobacteriosis in the same geographical area. Forty-eight combined fla types were seen among the 315 Campylobacter isolates studied. Six were found in isolates from all four hosts and represented 50% of the total number of isolates. Seventy-one different SmaI PFGE macrorestriction profiles (mrps) were observed, with 86% of isolates assigned to one of 29 different mrps. Fifty-seven isolates from diverse hosts, times, and sources had an identical SmaI mrp and combined fla type. Conversely, a number of genotypes were unique to a particular host. We provide molecular evidence which suggests a link between campylobacters in the farm environment with those causing disease in the community.

M3 - Journal article

VL - 67

SP - 1429

EP - 1436

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 4

ER -

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