Home > Research > Publications & Outputs > Time-resolved and two-photon emission imaging m...

Links

Text available via DOI:

View graph of relations

Time-resolved and two-photon emission imaging microscopy of live cells with inert platinum complexes

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

Time-resolved and two-photon emission imaging microscopy of live cells with inert platinum complexes. / Botchway, Stanley W; Charnley, Mirren; Haycock, John W et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 42, 21.10.2008, p. 16071-16076.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Botchway, SW, Charnley, M, Haycock, JW, Parker, AW, Rochester, DL, Weinstein, JA & Williams, JAG 2008, 'Time-resolved and two-photon emission imaging microscopy of live cells with inert platinum complexes', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 42, pp. 16071-16076. https://doi.org/10.1073/pnas.0804071105

APA

Botchway, S. W., Charnley, M., Haycock, J. W., Parker, A. W., Rochester, D. L., Weinstein, J. A., & Williams, J. A. G. (2008). Time-resolved and two-photon emission imaging microscopy of live cells with inert platinum complexes. Proceedings of the National Academy of Sciences of the United States of America, 105(42), 16071-16076. https://doi.org/10.1073/pnas.0804071105

Vancouver

Botchway SW, Charnley M, Haycock JW, Parker AW, Rochester DL, Weinstein JA et al. Time-resolved and two-photon emission imaging microscopy of live cells with inert platinum complexes. Proceedings of the National Academy of Sciences of the United States of America. 2008 Oct 21;105(42):16071-16076. doi: 10.1073/pnas.0804071105

Author

Botchway, Stanley W ; Charnley, Mirren ; Haycock, John W et al. / Time-resolved and two-photon emission imaging microscopy of live cells with inert platinum complexes. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 42. pp. 16071-16076.

Bibtex

@article{eb3cb1ee158d45f1aa8b7eb3d23abc8d,
title = "Time-resolved and two-photon emission imaging microscopy of live cells with inert platinum complexes",
abstract = "This work explores time-resolved emission imaging microscopy (TREM) for noninvasive imaging and mapping of live cells on a hitherto uncharted microsecond time scale. Simple robust molecules for this purpose have long been sought. We have developed highly emissive, synthetically versatile, and photostable platinum(II) complexes that make TREM a practicable reality. [PtLCl], {HL = 1,3-di(2-pyridyl)benzene and derivatives}, are charge-neutral, small molecules that have low cytotoxicity and accumulate intracellularly within a remarkably short incubation time of 5 min, apparently under diffusion control. Their microsecond lifetimes and emission quantum yields of up to 70% are exceptionally high for transition metal complexes and permit the application of TREM to be demonstrated in a range of live cell types-normal human dermal fibroblast, neoplastic C8161 and CHO cells. [PtLCl] are thus likely to be suitable emission labels for any eukaryotic cell types. The high photostability of [PtLCl] under intense prolonged irradiation has allowed the development of tissue-friendly NIR two-photon excitation (TPE) in conjunction with transition metal complexes in live cells. A combination of confocal one-photon excitation, nonlinear TPE, and microsecond time-resolved imaging has revealed (i) preferential localization of the complexes to intracellular nucleic acid structures, in particular the nucleoli and (ii) the possibility of measuring intracellular emission lifetimes in the microsecond range. The combination of TREM, TPE, and Pt(II) complexes will be a powerful tool for investigating intracellular processes in vivo, because the long lifetimes allow discrimination from autofluorescence and open up the use of commonplace technology.",
keywords = "Absorption, Animals, Cell Line, Cell Survival, Cricetinae, Humans, Kinetics, Microscopy, Fluorescence, Multiphoton/methods, Platinum Compounds, Time Factors",
author = "Botchway, {Stanley W} and Mirren Charnley and Haycock, {John W} and Parker, {Anthony W} and Rochester, {David L} and Weinstein, {Julia A} and Williams, {J A Gareth}",
year = "2008",
month = oct,
day = "21",
doi = "10.1073/pnas.0804071105",
language = "English",
volume = "105",
pages = "16071--16076",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "42",

}

RIS

TY - JOUR

T1 - Time-resolved and two-photon emission imaging microscopy of live cells with inert platinum complexes

AU - Botchway, Stanley W

AU - Charnley, Mirren

AU - Haycock, John W

AU - Parker, Anthony W

AU - Rochester, David L

AU - Weinstein, Julia A

AU - Williams, J A Gareth

PY - 2008/10/21

Y1 - 2008/10/21

N2 - This work explores time-resolved emission imaging microscopy (TREM) for noninvasive imaging and mapping of live cells on a hitherto uncharted microsecond time scale. Simple robust molecules for this purpose have long been sought. We have developed highly emissive, synthetically versatile, and photostable platinum(II) complexes that make TREM a practicable reality. [PtLCl], {HL = 1,3-di(2-pyridyl)benzene and derivatives}, are charge-neutral, small molecules that have low cytotoxicity and accumulate intracellularly within a remarkably short incubation time of 5 min, apparently under diffusion control. Their microsecond lifetimes and emission quantum yields of up to 70% are exceptionally high for transition metal complexes and permit the application of TREM to be demonstrated in a range of live cell types-normal human dermal fibroblast, neoplastic C8161 and CHO cells. [PtLCl] are thus likely to be suitable emission labels for any eukaryotic cell types. The high photostability of [PtLCl] under intense prolonged irradiation has allowed the development of tissue-friendly NIR two-photon excitation (TPE) in conjunction with transition metal complexes in live cells. A combination of confocal one-photon excitation, nonlinear TPE, and microsecond time-resolved imaging has revealed (i) preferential localization of the complexes to intracellular nucleic acid structures, in particular the nucleoli and (ii) the possibility of measuring intracellular emission lifetimes in the microsecond range. The combination of TREM, TPE, and Pt(II) complexes will be a powerful tool for investigating intracellular processes in vivo, because the long lifetimes allow discrimination from autofluorescence and open up the use of commonplace technology.

AB - This work explores time-resolved emission imaging microscopy (TREM) for noninvasive imaging and mapping of live cells on a hitherto uncharted microsecond time scale. Simple robust molecules for this purpose have long been sought. We have developed highly emissive, synthetically versatile, and photostable platinum(II) complexes that make TREM a practicable reality. [PtLCl], {HL = 1,3-di(2-pyridyl)benzene and derivatives}, are charge-neutral, small molecules that have low cytotoxicity and accumulate intracellularly within a remarkably short incubation time of 5 min, apparently under diffusion control. Their microsecond lifetimes and emission quantum yields of up to 70% are exceptionally high for transition metal complexes and permit the application of TREM to be demonstrated in a range of live cell types-normal human dermal fibroblast, neoplastic C8161 and CHO cells. [PtLCl] are thus likely to be suitable emission labels for any eukaryotic cell types. The high photostability of [PtLCl] under intense prolonged irradiation has allowed the development of tissue-friendly NIR two-photon excitation (TPE) in conjunction with transition metal complexes in live cells. A combination of confocal one-photon excitation, nonlinear TPE, and microsecond time-resolved imaging has revealed (i) preferential localization of the complexes to intracellular nucleic acid structures, in particular the nucleoli and (ii) the possibility of measuring intracellular emission lifetimes in the microsecond range. The combination of TREM, TPE, and Pt(II) complexes will be a powerful tool for investigating intracellular processes in vivo, because the long lifetimes allow discrimination from autofluorescence and open up the use of commonplace technology.

KW - Absorption

KW - Animals

KW - Cell Line

KW - Cell Survival

KW - Cricetinae

KW - Humans

KW - Kinetics

KW - Microscopy, Fluorescence, Multiphoton/methods

KW - Platinum Compounds

KW - Time Factors

U2 - 10.1073/pnas.0804071105

DO - 10.1073/pnas.0804071105

M3 - Journal article

C2 - 18852476

VL - 105

SP - 16071

EP - 16076

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 42

ER -