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Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation

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Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation. / Shahzadi, L.; Yar, M.; Jamal, A.; Siddiqi, S.A.; Chaudhry, A.A.; Zahid, S.; Tariq, M.; Rehman, I.U.; MacNeil, S.

In: JOURNAL OF BIOMATERIALS APPLICATIONS, Vol. 31, No. 4, 01.06.2016, p. 582-593.

Research output: Contribution to journalJournal article

Harvard

Shahzadi, L, Yar, M, Jamal, A, Siddiqi, SA, Chaudhry, AA, Zahid, S, Tariq, M, Rehman, IU & MacNeil, S 2016, 'Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation', JOURNAL OF BIOMATERIALS APPLICATIONS, vol. 31, no. 4, pp. 582-593. https://doi.org/10.1177/0885328216650125

APA

Shahzadi, L., Yar, M., Jamal, A., Siddiqi, S. A., Chaudhry, A. A., Zahid, S., Tariq, M., Rehman, I. U., & MacNeil, S. (2016). Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation. JOURNAL OF BIOMATERIALS APPLICATIONS, 31(4), 582-593. https://doi.org/10.1177/0885328216650125

Vancouver

Author

Shahzadi, L. ; Yar, M. ; Jamal, A. ; Siddiqi, S.A. ; Chaudhry, A.A. ; Zahid, S. ; Tariq, M. ; Rehman, I.U. ; MacNeil, S. / Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation. In: JOURNAL OF BIOMATERIALS APPLICATIONS. 2016 ; Vol. 31, No. 4. pp. 582-593.

Bibtex

@article{7a59020404fa464f879c6a89bbeaa518,
title = "Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation",
abstract = "There is a need to develop pro-angiogenic biomaterials to promote wound healing and to assist in regenerative medicine. To this end, various growth factors have been exploited which have the potential to promote angiogenesis. However, these are generally expensive and labile which limits their effectiveness. An alternative approach is to immobilize heparin onto biocompatible degradable hydrogels. The heparin in turn will then bind endogenous proangiogenic growth factors to induce formation of new blood vessels. In this study, we continue our development of hydrogels for wound healing purposes by exploring covalently cross-linking chitosan and polyvinyl alcohol hydrogels using triethyl orthoformate. Two concentrations of triethyl orthoformate (4 and 16%) were compared for their effects on the structure of hydrogels - their swelling, pore size, and rate of degradation and for their ability to support the growth of cells and for their heparin-binding capacity and their effects on angiogenesis in a chick chorioallantoic membrane assay. Hydrogels formed with 4 or 16% both triethyl orthoformate cross-linker were equally cyto-compatible. Hydrogels formed with 4% triethyl orthoformate absorbed slightly more water than those made with 16% triethyl orthoformate and broke down slightly faster than non-cross-linked hydrogels. When soaked in heparin the hydrogel formed with 16% triethyl orthoformate showed more blood vessel formation in the CAM assay than that formed with 4% triethyl orthoformate. {\textcopyright} The Author(s) 2016.",
keywords = "angiogenesis, Chitosan, cross-linked chitosan, heparin, hydrogels, Bins, Biocompatibility, Blood vessels, Chitin, Crosslinking, Polysaccharides, Pore size, Angiogenesis, Biodegradable scaffold, Chick chorioallantoic membrane, Covalently cross-linked, Cross linked chitosan, Cross-linked hydrogels, Regenerative medicine, Hydrogels, chemical compound, chitosan, molecular scaffold, polyvinyl alcohol, tolonium chloride, triethyl orthoformate, unclassified drug, cross linking reagent, formic acid derivative, protein binding, animal tissue, Article, binding affinity, biocompatibility, biodegradability, cell growth, chorioallantoic membrane assay, concentration (parameters), controlled study, covalent bond, cross linking, degradation kinetics, human, human cell, hydrogel, nonhuman, particle size, priority journal, structure analysis, thermal analysis, adsorption, animal, biodegradable implant, blood vessel, chemistry, chick embryo, chorioallantois, cytology, device failure analysis, devices, equipment design, growth, development and aging, materials testing, physiology, procedures, synthesis, tissue engineering, tissue scaffold, vascularization, Absorbable Implants, Adsorption, Animals, Blood Vessels, Chick Embryo, Chorioallantoic Membrane, Cross-Linking Reagents, Equipment Design, Equipment Failure Analysis, Formates, Heparin, Materials Testing, Neovascularization, Physiologic, Polyvinyl Alcohol, Protein Binding, Tissue Engineering, Tissue Scaffolds",
author = "L. Shahzadi and M. Yar and A. Jamal and S.A. Siddiqi and A.A. Chaudhry and S. Zahid and M. Tariq and I.U. Rehman and S. MacNeil",
year = "2016",
month = jun
day = "1",
doi = "10.1177/0885328216650125",
language = "English",
volume = "31",
pages = "582--593",
journal = "JOURNAL OF BIOMATERIALS APPLICATIONS",
issn = "0885-3282",
publisher = "SAGE Publications Ltd",
number = "4",

}

RIS

TY - JOUR

T1 - Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation

AU - Shahzadi, L.

AU - Yar, M.

AU - Jamal, A.

AU - Siddiqi, S.A.

AU - Chaudhry, A.A.

AU - Zahid, S.

AU - Tariq, M.

AU - Rehman, I.U.

AU - MacNeil, S.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - There is a need to develop pro-angiogenic biomaterials to promote wound healing and to assist in regenerative medicine. To this end, various growth factors have been exploited which have the potential to promote angiogenesis. However, these are generally expensive and labile which limits their effectiveness. An alternative approach is to immobilize heparin onto biocompatible degradable hydrogels. The heparin in turn will then bind endogenous proangiogenic growth factors to induce formation of new blood vessels. In this study, we continue our development of hydrogels for wound healing purposes by exploring covalently cross-linking chitosan and polyvinyl alcohol hydrogels using triethyl orthoformate. Two concentrations of triethyl orthoformate (4 and 16%) were compared for their effects on the structure of hydrogels - their swelling, pore size, and rate of degradation and for their ability to support the growth of cells and for their heparin-binding capacity and their effects on angiogenesis in a chick chorioallantoic membrane assay. Hydrogels formed with 4 or 16% both triethyl orthoformate cross-linker were equally cyto-compatible. Hydrogels formed with 4% triethyl orthoformate absorbed slightly more water than those made with 16% triethyl orthoformate and broke down slightly faster than non-cross-linked hydrogels. When soaked in heparin the hydrogel formed with 16% triethyl orthoformate showed more blood vessel formation in the CAM assay than that formed with 4% triethyl orthoformate. © The Author(s) 2016.

AB - There is a need to develop pro-angiogenic biomaterials to promote wound healing and to assist in regenerative medicine. To this end, various growth factors have been exploited which have the potential to promote angiogenesis. However, these are generally expensive and labile which limits their effectiveness. An alternative approach is to immobilize heparin onto biocompatible degradable hydrogels. The heparin in turn will then bind endogenous proangiogenic growth factors to induce formation of new blood vessels. In this study, we continue our development of hydrogels for wound healing purposes by exploring covalently cross-linking chitosan and polyvinyl alcohol hydrogels using triethyl orthoformate. Two concentrations of triethyl orthoformate (4 and 16%) were compared for their effects on the structure of hydrogels - their swelling, pore size, and rate of degradation and for their ability to support the growth of cells and for their heparin-binding capacity and their effects on angiogenesis in a chick chorioallantoic membrane assay. Hydrogels formed with 4 or 16% both triethyl orthoformate cross-linker were equally cyto-compatible. Hydrogels formed with 4% triethyl orthoformate absorbed slightly more water than those made with 16% triethyl orthoformate and broke down slightly faster than non-cross-linked hydrogels. When soaked in heparin the hydrogel formed with 16% triethyl orthoformate showed more blood vessel formation in the CAM assay than that formed with 4% triethyl orthoformate. © The Author(s) 2016.

KW - angiogenesis

KW - Chitosan

KW - cross-linked chitosan

KW - heparin

KW - hydrogels

KW - Bins

KW - Biocompatibility

KW - Blood vessels

KW - Chitin

KW - Crosslinking

KW - Polysaccharides

KW - Pore size

KW - Angiogenesis

KW - Biodegradable scaffold

KW - Chick chorioallantoic membrane

KW - Covalently cross-linked

KW - Cross linked chitosan

KW - Cross-linked hydrogels

KW - Regenerative medicine

KW - Hydrogels

KW - chemical compound

KW - chitosan

KW - molecular scaffold

KW - polyvinyl alcohol

KW - tolonium chloride

KW - triethyl orthoformate

KW - unclassified drug

KW - cross linking reagent

KW - formic acid derivative

KW - protein binding

KW - animal tissue

KW - Article

KW - binding affinity

KW - biocompatibility

KW - biodegradability

KW - cell growth

KW - chorioallantoic membrane assay

KW - concentration (parameters)

KW - controlled study

KW - covalent bond

KW - cross linking

KW - degradation kinetics

KW - human

KW - human cell

KW - hydrogel

KW - nonhuman

KW - particle size

KW - priority journal

KW - structure analysis

KW - thermal analysis

KW - adsorption

KW - animal

KW - biodegradable implant

KW - blood vessel

KW - chemistry

KW - chick embryo

KW - chorioallantois

KW - cytology

KW - device failure analysis

KW - devices

KW - equipment design

KW - growth, development and aging

KW - materials testing

KW - physiology

KW - procedures

KW - synthesis

KW - tissue engineering

KW - tissue scaffold

KW - vascularization

KW - Absorbable Implants

KW - Adsorption

KW - Animals

KW - Blood Vessels

KW - Chick Embryo

KW - Chorioallantoic Membrane

KW - Cross-Linking Reagents

KW - Equipment Design

KW - Equipment Failure Analysis

KW - Formates

KW - Heparin

KW - Materials Testing

KW - Neovascularization, Physiologic

KW - Polyvinyl Alcohol

KW - Protein Binding

KW - Tissue Engineering

KW - Tissue Scaffolds

U2 - 10.1177/0885328216650125

DO - 10.1177/0885328216650125

M3 - Journal article

VL - 31

SP - 582

EP - 593

JO - JOURNAL OF BIOMATERIALS APPLICATIONS

JF - JOURNAL OF BIOMATERIALS APPLICATIONS

SN - 0885-3282

IS - 4

ER -