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Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagella membrane formation.

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Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagella membrane formation. / Davidge, Jacqueline A.; Shawcross, Emma; Dickinson, Harriet A. et al.
In: Journal of Cell Science, Vol. 119, No. 19, 2006, p. 3935-3943.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

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Davidge JA, Shawcross E, Dickinson HA, Towers K, Ginger ML, McKean PG et al. Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagella membrane formation. Journal of Cell Science. 2006;119(19):3935-3943. doi: 10.1242/jcs.03203

Author

Davidge, Jacqueline A. ; Shawcross, Emma ; Dickinson, Harriet A. et al. / Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagella membrane formation. In: Journal of Cell Science. 2006 ; Vol. 119, No. 19. pp. 3935-3943.

Bibtex

@article{d9d8bba614e2425a9ef4070009f8c335,
title = "Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagella membrane formation.",
abstract = "The flagella connector (FC) of procyclic trypanosomes is a mobile, transmembrane junction important in providing cytotactic morphogenetic information to the daughter cell. Quantitative analyses of FC positioning along the old flagellum, involving direct observations and use of the MPM2 anti-phosphoprotein monoclonal reveals a `stop point' is reached on the old flagellum which correlates well with the initiation of basal body migration and kinetoplast segregation. This demonstrates further complexities of the FC and its movement in morphogenetic events in trypanosomes than have hitherto been described. We used intraflagellar transport RNAi mutants to ablate the formation of a new flagellum. Intriguingly the FC could still move, indicating that a motor function beyond the new flagellum is sufficient to move it. When such a FC moves, it drags a sleeve of new flagellar membrane out of the flagellar pocket. This axoneme-less flagellar membrane maintains appropriate developmental relationships to the cell body including following the correct helical path and being connected to the internal cytoskeleton by macula adherens junctions. Movement of the FC in the apparent absence of intraflagellar transport raises the possibility of a new form of motility within a eukaryotic flagellum.",
keywords = "Trypanosome, Morphogenesis, Flagellar connector, Phosphorylation, Basal body",
author = "Davidge, {Jacqueline A.} and Emma Shawcross and Dickinson, {Harriet A.} and Katie Towers and Ginger, {Michael L.} and McKean, {Paul G.} and Keith Gull",
year = "2006",
doi = "10.1242/jcs.03203",
language = "English",
volume = "119",
pages = "3935--3943",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "19",

}

RIS

TY - JOUR

T1 - Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagella membrane formation.

AU - Davidge, Jacqueline A.

AU - Shawcross, Emma

AU - Dickinson, Harriet A.

AU - Towers, Katie

AU - Ginger, Michael L.

AU - McKean, Paul G.

AU - Gull, Keith

PY - 2006

Y1 - 2006

N2 - The flagella connector (FC) of procyclic trypanosomes is a mobile, transmembrane junction important in providing cytotactic morphogenetic information to the daughter cell. Quantitative analyses of FC positioning along the old flagellum, involving direct observations and use of the MPM2 anti-phosphoprotein monoclonal reveals a `stop point' is reached on the old flagellum which correlates well with the initiation of basal body migration and kinetoplast segregation. This demonstrates further complexities of the FC and its movement in morphogenetic events in trypanosomes than have hitherto been described. We used intraflagellar transport RNAi mutants to ablate the formation of a new flagellum. Intriguingly the FC could still move, indicating that a motor function beyond the new flagellum is sufficient to move it. When such a FC moves, it drags a sleeve of new flagellar membrane out of the flagellar pocket. This axoneme-less flagellar membrane maintains appropriate developmental relationships to the cell body including following the correct helical path and being connected to the internal cytoskeleton by macula adherens junctions. Movement of the FC in the apparent absence of intraflagellar transport raises the possibility of a new form of motility within a eukaryotic flagellum.

AB - The flagella connector (FC) of procyclic trypanosomes is a mobile, transmembrane junction important in providing cytotactic morphogenetic information to the daughter cell. Quantitative analyses of FC positioning along the old flagellum, involving direct observations and use of the MPM2 anti-phosphoprotein monoclonal reveals a `stop point' is reached on the old flagellum which correlates well with the initiation of basal body migration and kinetoplast segregation. This demonstrates further complexities of the FC and its movement in morphogenetic events in trypanosomes than have hitherto been described. We used intraflagellar transport RNAi mutants to ablate the formation of a new flagellum. Intriguingly the FC could still move, indicating that a motor function beyond the new flagellum is sufficient to move it. When such a FC moves, it drags a sleeve of new flagellar membrane out of the flagellar pocket. This axoneme-less flagellar membrane maintains appropriate developmental relationships to the cell body including following the correct helical path and being connected to the internal cytoskeleton by macula adherens junctions. Movement of the FC in the apparent absence of intraflagellar transport raises the possibility of a new form of motility within a eukaryotic flagellum.

KW - Trypanosome

KW - Morphogenesis

KW - Flagellar connector

KW - Phosphorylation

KW - Basal body

U2 - 10.1242/jcs.03203

DO - 10.1242/jcs.03203

M3 - Journal article

VL - 119

SP - 3935

EP - 3943

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 19

ER -