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Two Cytosolic Glutamine Synthetase Isoforms of Maize Are Specifically Involved in the Control of Grain Production.

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Published

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Two Cytosolic Glutamine Synthetase Isoforms of Maize Are Specifically Involved in the Control of Grain Production. / Martin, Antoine; Lee, Judy; Kichey, Thomas et al.
In: Plant Cell, Vol. 18, No. 11, 2006, p. 3252-3274.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Martin, A, Lee, J, Kichey, T, Gerentes, D, Zivy, M, Tatout, C, Dubois, F, Balliau, T, Valot, B, Davanture, M, Tercé-Laforgue, T, Quilleré, I, Coque, M, Gallais, A, Gonzalez-Moro, M-B, Bethencourt, L, Habash, DZ, Lea, PJ, Charcosset, A, Perez, P, Murigneux, A, Sakakibara, H, Edwards, KJ & Hirel, B 2006, 'Two Cytosolic Glutamine Synthetase Isoforms of Maize Are Specifically Involved in the Control of Grain Production.', Plant Cell, vol. 18, no. 11, pp. 3252-3274. https://doi.org/10.1105/tpc.106.042689

APA

Martin, A., Lee, J., Kichey, T., Gerentes, D., Zivy, M., Tatout, C., Dubois, F., Balliau, T., Valot, B., Davanture, M., Tercé-Laforgue, T., Quilleré, I., Coque, M., Gallais, A., Gonzalez-Moro, M-B., Bethencourt, L., Habash, D. Z., Lea, P. J., Charcosset, A., ... Hirel, B. (2006). Two Cytosolic Glutamine Synthetase Isoforms of Maize Are Specifically Involved in the Control of Grain Production. Plant Cell, 18(11), 3252-3274. https://doi.org/10.1105/tpc.106.042689

Vancouver

Martin A, Lee J, Kichey T, Gerentes D, Zivy M, Tatout C et al. Two Cytosolic Glutamine Synthetase Isoforms of Maize Are Specifically Involved in the Control of Grain Production. Plant Cell. 2006;18(11):3252-3274. doi: 10.1105/tpc.106.042689

Author

Martin, Antoine ; Lee, Judy ; Kichey, Thomas et al. / Two Cytosolic Glutamine Synthetase Isoforms of Maize Are Specifically Involved in the Control of Grain Production. In: Plant Cell. 2006 ; Vol. 18, No. 11. pp. 3252-3274.

Bibtex

@article{3dac8066b7564b02a1a3239a4e4db64d,
title = "Two Cytosolic Glutamine Synthetase Isoforms of Maize Are Specifically Involved in the Control of Grain Production.",
abstract = "The roles of two cytosolic maize glutamine synthetase isoenzymes (GS1), products of the Gln1-3 and Gln1-4 genes, were investigated by examining the impact of knockout mutations on kernel yield. In the gln1-3 and gln1-4 single mutants and the gln1-3 gln1-4 double mutant, GS mRNA expression was impaired, resulting in reduced GS1 protein and activity. The gln1-4 phenotype displayed reduced kernel size and gln1-3 reduced kernel number, with both phenotypes displayed in gln1-3 gln1-4. However, at maturity, shoot biomass production was not modified in either the single mutants or double mutants, suggesting a specific impact on grain production in both mutants. Asn increased in the leaves of the mutants during grain filling, indicating that it probably accumulates to circumvent ammonium buildup resulting from lower GS1 activity. Phloem sap analysis revealed that unlike Gln, Asn is not efficiently transported to developing kernels, apparently causing reduced kernel production. When Gln1-3 was overexpressed constitutively in leaves, kernel number increased by 30%, providing further evidence that GS1-3 plays a major role in kernel yield. Cytoimmunochemistry and in situ hybridization revealed that GS1-3 is present in mesophyll cells, whereas GS1-4 is specifically localized in the bundle sheath cells. The two GS1 isoenzymes play nonredundant roles with respect to their tissue-specific localization.",
author = "Antoine Martin and Judy Lee and Thomas Kichey and Denise Gerentes and Michel Zivy and Christophe Tatout and Fr{\'e}d{\'e}ric Dubois and Thierry Balliau and Beno{\^i}t Valot and Marl{\`e}ne Davanture and Th{\'e}r{\`e}se Terc{\'e}-Laforgue and Isabelle Quiller{\'e} and Marie Coque and Andr{\'e} Gallais and Mar{\'i}a-Bego{\~n}a Gonzalez-Moro and Linda Bethencourt and Habash, {Dimah Z.} and Lea, {Peter J.} and Alain Charcosset and Pascual Perez and Alain Murigneux and Hitoshi Sakakibara and Edwards, {Keith J.} and Bertrand Hirel",
year = "2006",
doi = "10.1105/tpc.106.042689",
language = "English",
volume = "18",
pages = "3252--3274",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "11",

}

RIS

TY - JOUR

T1 - Two Cytosolic Glutamine Synthetase Isoforms of Maize Are Specifically Involved in the Control of Grain Production.

AU - Martin, Antoine

AU - Lee, Judy

AU - Kichey, Thomas

AU - Gerentes, Denise

AU - Zivy, Michel

AU - Tatout, Christophe

AU - Dubois, Frédéric

AU - Balliau, Thierry

AU - Valot, Benoît

AU - Davanture, Marlène

AU - Tercé-Laforgue, Thérèse

AU - Quilleré, Isabelle

AU - Coque, Marie

AU - Gallais, André

AU - Gonzalez-Moro, María-Begoña

AU - Bethencourt, Linda

AU - Habash, Dimah Z.

AU - Lea, Peter J.

AU - Charcosset, Alain

AU - Perez, Pascual

AU - Murigneux, Alain

AU - Sakakibara, Hitoshi

AU - Edwards, Keith J.

AU - Hirel, Bertrand

PY - 2006

Y1 - 2006

N2 - The roles of two cytosolic maize glutamine synthetase isoenzymes (GS1), products of the Gln1-3 and Gln1-4 genes, were investigated by examining the impact of knockout mutations on kernel yield. In the gln1-3 and gln1-4 single mutants and the gln1-3 gln1-4 double mutant, GS mRNA expression was impaired, resulting in reduced GS1 protein and activity. The gln1-4 phenotype displayed reduced kernel size and gln1-3 reduced kernel number, with both phenotypes displayed in gln1-3 gln1-4. However, at maturity, shoot biomass production was not modified in either the single mutants or double mutants, suggesting a specific impact on grain production in both mutants. Asn increased in the leaves of the mutants during grain filling, indicating that it probably accumulates to circumvent ammonium buildup resulting from lower GS1 activity. Phloem sap analysis revealed that unlike Gln, Asn is not efficiently transported to developing kernels, apparently causing reduced kernel production. When Gln1-3 was overexpressed constitutively in leaves, kernel number increased by 30%, providing further evidence that GS1-3 plays a major role in kernel yield. Cytoimmunochemistry and in situ hybridization revealed that GS1-3 is present in mesophyll cells, whereas GS1-4 is specifically localized in the bundle sheath cells. The two GS1 isoenzymes play nonredundant roles with respect to their tissue-specific localization.

AB - The roles of two cytosolic maize glutamine synthetase isoenzymes (GS1), products of the Gln1-3 and Gln1-4 genes, were investigated by examining the impact of knockout mutations on kernel yield. In the gln1-3 and gln1-4 single mutants and the gln1-3 gln1-4 double mutant, GS mRNA expression was impaired, resulting in reduced GS1 protein and activity. The gln1-4 phenotype displayed reduced kernel size and gln1-3 reduced kernel number, with both phenotypes displayed in gln1-3 gln1-4. However, at maturity, shoot biomass production was not modified in either the single mutants or double mutants, suggesting a specific impact on grain production in both mutants. Asn increased in the leaves of the mutants during grain filling, indicating that it probably accumulates to circumvent ammonium buildup resulting from lower GS1 activity. Phloem sap analysis revealed that unlike Gln, Asn is not efficiently transported to developing kernels, apparently causing reduced kernel production. When Gln1-3 was overexpressed constitutively in leaves, kernel number increased by 30%, providing further evidence that GS1-3 plays a major role in kernel yield. Cytoimmunochemistry and in situ hybridization revealed that GS1-3 is present in mesophyll cells, whereas GS1-4 is specifically localized in the bundle sheath cells. The two GS1 isoenzymes play nonredundant roles with respect to their tissue-specific localization.

U2 - 10.1105/tpc.106.042689

DO - 10.1105/tpc.106.042689

M3 - Journal article

VL - 18

SP - 3252

EP - 3274

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 11

ER -