Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo

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Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo. / Kadioglu, A; Sharpe, J A; Lazou, I et al.
In: FEMS Microbiology Letters, Vol. 194, No. 1, 01.2001, p. 105-10.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Kadioglu, A, Sharpe, JA, Lazou, I, Svanborg, C, Ockleford, C, Mitchell, TJ & Andrew, PW 2001, 'Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo', FEMS Microbiology Letters, vol. 194, no. 1, pp. 105-10. https://doi.org/10.1111/j.1574-6968.2001.tb09454.x

APA

Kadioglu, A., Sharpe, J. A., Lazou, I., Svanborg, C., Ockleford, C., Mitchell, T. J., & Andrew, P. W. (2001). Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo. FEMS Microbiology Letters, 194(1), 105-10. https://doi.org/10.1111/j.1574-6968.2001.tb09454.x

Vancouver

Kadioglu A, Sharpe JA, Lazou I, Svanborg C, Ockleford C, Mitchell TJ et al. Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo. FEMS Microbiology Letters. 2001 Jan;194(1):105-10. doi: 10.1111/j.1574-6968.2001.tb09454.x

Author

Kadioglu, A ; Sharpe, J A ; Lazou, I et al. / Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo. In: FEMS Microbiology Letters. 2001 ; Vol. 194, No. 1. pp. 105-10.

Bibtex

@article{6ff9ae6fcbcc47c7bcc37ba8475379e4,

title = "Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo",

abstract = "The pneumococcus is the principle cause of bacterial pneumonia and also a major cause of bacterial meningitis. The mechanisms and sites of pneumococcal adherence and invasion of the respiratory tract in vivo are not clear however. We have made pneumococci expressing green fluorescent protein (GFP) and used it to trace pneumococcal adherence and invasion in vivo. By using GFP pneumococci we have shown bacterial adherence and invasion of broncho-epithelial cells in vivo by 4 h post-infection, with increases in pneumococcal invasiveness by 24 h. Using confocal image analysis we have shown varying levels of pneumococcal penetration and internalisation into host cells, as well as translocation through epithelial layers. To our knowledge this is the first report of pneumococcal invasion and cellular translocation in vivo.",

keywords = "Animals, Bacterial Adhesion, Blood, Bronchi, Epithelial Cells, Female, Green Fluorescent Proteins, Luminescent Proteins, Lung, Mice, Microscopy, Confocal, Plasmids, Pneumococcal Infections, Streptococcus pneumoniae, Transformation, Bacterial",

author = "A Kadioglu and Sharpe, {J A} and I Lazou and C Svanborg and C Ockleford and Mitchell, {T J} and Andrew, {P W}",

year = "2001",

month = jan,

doi = "10.1111/j.1574-6968.2001.tb09454.x",

language = "English",

volume = "194",

pages = "105--10",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Wiley-Blackwell",

number = "1",

}

RIS

TY - JOUR

T1 - Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo

AU - Kadioglu, A

AU - Sharpe, J A

AU - Lazou, I

AU - Svanborg, C

AU - Ockleford, C

AU - Mitchell, T J

AU - Andrew, P W

PY - 2001/1

Y1 - 2001/1

N2 - The pneumococcus is the principle cause of bacterial pneumonia and also a major cause of bacterial meningitis. The mechanisms and sites of pneumococcal adherence and invasion of the respiratory tract in vivo are not clear however. We have made pneumococci expressing green fluorescent protein (GFP) and used it to trace pneumococcal adherence and invasion in vivo. By using GFP pneumococci we have shown bacterial adherence and invasion of broncho-epithelial cells in vivo by 4 h post-infection, with increases in pneumococcal invasiveness by 24 h. Using confocal image analysis we have shown varying levels of pneumococcal penetration and internalisation into host cells, as well as translocation through epithelial layers. To our knowledge this is the first report of pneumococcal invasion and cellular translocation in vivo.

AB - The pneumococcus is the principle cause of bacterial pneumonia and also a major cause of bacterial meningitis. The mechanisms and sites of pneumococcal adherence and invasion of the respiratory tract in vivo are not clear however. We have made pneumococci expressing green fluorescent protein (GFP) and used it to trace pneumococcal adherence and invasion in vivo. By using GFP pneumococci we have shown bacterial adherence and invasion of broncho-epithelial cells in vivo by 4 h post-infection, with increases in pneumococcal invasiveness by 24 h. Using confocal image analysis we have shown varying levels of pneumococcal penetration and internalisation into host cells, as well as translocation through epithelial layers. To our knowledge this is the first report of pneumococcal invasion and cellular translocation in vivo.

KW - Animals

KW - Bacterial Adhesion

KW - Blood

KW - Bronchi

KW - Epithelial Cells

KW - Female

KW - Green Fluorescent Proteins

KW - Luminescent Proteins

KW - Lung

KW - Mice

KW - Microscopy, Confocal

KW - Plasmids

KW - Pneumococcal Infections

KW - Streptococcus pneumoniae

KW - Transformation, Bacterial

U2 - 10.1111/j.1574-6968.2001.tb09454.x

DO - 10.1111/j.1574-6968.2001.tb09454.x

M3 - Journal article

C2 - 11150674

VL - 194

SP - 105

EP - 110

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 1

ER -

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