TY - JOUR

T1 - Use of tissue ink to maintain identification of individual cores on needle biopsies of the prostate.

AU - Singh, P. B.

AU - Saw, N. K.

AU - Haq, A.

AU - Blades, R. A.

AU - Martin, Frank L.

AU - Matanhelia, S. S.

AU - Nicholson, C. M.

PY - 2008/9

Y1 - 2008/9

N2 - Background: There is an increasing necessity to extract the maximum amount of information, beyond even a cancer diagnosis, from prostate biopsies. Thus, maintaining site-specific information regarding individual biopsy cores might be critical. Aim: To evaluate the applicability of employing tissue ink to maintain the identity of individual prostatic biopsy cores. Method: In this ongoing study, 12 core prostate biopsy specimens are sent to the laboratory in individual pots labelled according to anatomical site. The specimens are placed in two separate multi-compartment cassettes. They are inked with different colours to identify the site of origin from each lobe. The cassettes are then processed with a single paraffin block for each side; the six cores from each side can be mounted on a single slide. Results: The different colours used adhere well to the biopsy cores, thus maintaining the identity of each core. Six cores from each side are embedded in a single paraffin block and examined on a single slide, making it cost-effective, while maintaining high quality, accurate histopathological information. Conclusion: Differential inking of prostate biopsy cores is an easily applicable method that is cost-effective and provides tumour location information. Prostate biopsy data archived to maintain individual core information might be used to determine applicability of such information to predict extra-capsular extension by correlating with imaging and radical prostatectomy findings, and for treatment planning.

AB - Background: There is an increasing necessity to extract the maximum amount of information, beyond even a cancer diagnosis, from prostate biopsies. Thus, maintaining site-specific information regarding individual biopsy cores might be critical. Aim: To evaluate the applicability of employing tissue ink to maintain the identity of individual prostatic biopsy cores. Method: In this ongoing study, 12 core prostate biopsy specimens are sent to the laboratory in individual pots labelled according to anatomical site. The specimens are placed in two separate multi-compartment cassettes. They are inked with different colours to identify the site of origin from each lobe. The cassettes are then processed with a single paraffin block for each side; the six cores from each side can be mounted on a single slide. Results: The different colours used adhere well to the biopsy cores, thus maintaining the identity of each core. Six cores from each side are embedded in a single paraffin block and examined on a single slide, making it cost-effective, while maintaining high quality, accurate histopathological information. Conclusion: Differential inking of prostate biopsy cores is an easily applicable method that is cost-effective and provides tumour location information. Prostate biopsy data archived to maintain individual core information might be used to determine applicability of such information to predict extra-capsular extension by correlating with imaging and radical prostatectomy findings, and for treatment planning.

U2 - 10.1136/jcp.2008.058545

DO - 10.1136/jcp.2008.058545

M3 - Journal article

VL - 61

SP - 1055

EP - 1057

JO - Journal of Clinical Pathology

JF - Journal of Clinical Pathology

SN - 1472-4146

IS - 9

ER -