12,000

We have over 12,000 students, from over 100 countries, within one of the safest campuses in the UK

93%

93% of Lancaster students go into work or further study within six months of graduating

Home > Research > Publications & Outputs > Using infrared thermography to study the CO2 si...
View graph of relations

« Back

Using infrared thermography to study the CO2 signalling pathway in Arabidopsis.

Research output: Contribution to journalJournal article

Published

Journal publication date07/2005
JournalComparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology
Volume141
PagesS274
Original languageEnglish

Abstract

Stomata are small pores on leaf surfaces that regulate gas exchange. When stomata are closed evapotranspiration is reduced. This means that the temperature of leaves with closed stomata is warmer than leaves with open stomata. It is possible to visualise these temperature differences with an infrared thermal imaging camera. We are currently using this approach to isolate Arabidopsis stomatal CO2 response mutants. To do this we built a Teflon covered chamber that allows plants to be imaged from the outside. Once plants are inside the chamber we recorded thermal images during a period at ambient [CO2] (360 ppm) followed by a period at elevated [CO2] (up to 1400 ppm). Results showed that leaf temperature of 25 to 35 days old wild type plants increased 1–2 -C following exposure to elevated CO2. We screened around 18,000 EMS plants and plants that did not show the characteristic response to high CO2 were selected and seed collected. We are currently characterizing and analysing these putative mutants.

Bibliographic note

Abstracts of the Annual Main Meeting of the Society for Experimental Biology, 11th-15th July 2005, Universitat Autonoma de Barcelona, Barcelona, Spain