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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Using in-gel digestion and an Orbitrap mass spectrometer to analyze the proteome of clathrin-coated vesicles
AU - Borner, Georg H H
AU - Fielding, Andrew B.
N1 - © 2014 Cold Spring Harbor Laboratory Press.
PY - 2014/11/3
Y1 - 2014/11/3
N2 - The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by tryptic in-gel digest and peptide extraction followed by analysis in an Orbitrap mass spectrometer.
AB - The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by tryptic in-gel digest and peptide extraction followed by analysis in an Orbitrap mass spectrometer.
KW - Cell Fractionation
KW - Clathrin-Coated Vesicles
KW - Gels
KW - HeLa Cells
KW - Humans
KW - Mass Spectrometry
KW - Proteolysis
KW - Proteome
KW - Trypsin
KW - Journal Article
U2 - 10.1101/pdb.prot083154
DO - 10.1101/pdb.prot083154
M3 - Journal article
C2 - 25368313
VL - 2014
SP - 1188
EP - 1191
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
SN - 1940-3402
IS - 11
ER -