Xylem sap calcium concentrations do not explain liming-induced inhibition of legume gas exchange

Lancaster Environment Centre

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https://doi.org/10.1007/s11104-014-2118-5
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Keywords

Calcium, Lime, Pisum sativum, Phaseolus vulgaris, Stomatal conductance, Xylem sap, STOMATAL CONDUCTANCE, ABSCISIC-ACID, SOIL ACIDIFICATION, RICINUS-COMMUNIS, FLOW-RATE, WATER, PLANTS, SHOOT, LIME, TRANSPIRATION

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Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Xylem sap calcium concentrations do not explain liming-induced inhibition of legume gas exchange. / Rothwell, Shane A.; Dodd, Ian C.
In: Plant and Soil, Vol. 382, No. 1-2, 09.2014, p. 17-30.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{052f46df474f4e6fbb3db12b8d9465a7,

title = "Xylem sap calcium concentrations do not explain liming-induced inhibition of legume gas exchange",

abstract = "Liming is considered normal agricultural practise for remediating soil acidity and improving crop productivity; however recommended lime applications can reduce yield. We tested the hypothesis that elevated xylem sap Ca2+ limited gas exchange of Phaseolus vulgaris L. and Pisum sativum L. plants that exhibited reduced shoot biomass and leaf area when limed.We used Scholander and whole-plant pressure chamber techniques to collect root and leaf xylem sap, a calcium-specific ion-selective electrode to measure xylem sap Ca2+, infra-red gas analysis to measure gas exchange of limed and unlimed (control) plants, and a detached leaf transpiration bioassay to determine stomatal sensitivity to Ca2+.Liming reduced shoot biomass, leaf area and leaf gas exchange in both species. Root xylem sap Ca2+ concentration was only increased in P. vulgaris and not in P. sativum. Detached leaves of both species required 5 mM Ca2+ supplied to via the transpiration stream to induce stomatal closure, however, maximum in vivo xylem sap Ca2+ concentrations of limed plants was only 1.7 mM and thus not high enough to influence stomata.We conclude that an alternative xylem-borne antitranspirant other than Ca2+ decreases gas exchange of limed plants.",

keywords = "Calcium, Lime, Pisum sativum, Phaseolus vulgaris, Stomatal conductance, Xylem sap, STOMATAL CONDUCTANCE, ABSCISIC-ACID, SOIL ACIDIFICATION, RICINUS-COMMUNIS, FLOW-RATE, WATER, PLANTS, SHOOT, LIME, TRANSPIRATION",

author = "Rothwell, {Shane A.} and Dodd, {Ian C.}",

year = "2014",

month = sep,

doi = "10.1007/s11104-014-2118-5",

language = "English",

volume = "382",

pages = "17--30",

journal = "Plant and Soil",

issn = "0032-079X",

publisher = "Springer International Publishing AG",

number = "1-2",

}

RIS

TY - JOUR

T1 - Xylem sap calcium concentrations do not explain liming-induced inhibition of legume gas exchange

AU - Rothwell, Shane A.

AU - Dodd, Ian C.

PY - 2014/9

Y1 - 2014/9

N2 - Liming is considered normal agricultural practise for remediating soil acidity and improving crop productivity; however recommended lime applications can reduce yield. We tested the hypothesis that elevated xylem sap Ca2+ limited gas exchange of Phaseolus vulgaris L. and Pisum sativum L. plants that exhibited reduced shoot biomass and leaf area when limed.We used Scholander and whole-plant pressure chamber techniques to collect root and leaf xylem sap, a calcium-specific ion-selective electrode to measure xylem sap Ca2+, infra-red gas analysis to measure gas exchange of limed and unlimed (control) plants, and a detached leaf transpiration bioassay to determine stomatal sensitivity to Ca2+.Liming reduced shoot biomass, leaf area and leaf gas exchange in both species. Root xylem sap Ca2+ concentration was only increased in P. vulgaris and not in P. sativum. Detached leaves of both species required 5 mM Ca2+ supplied to via the transpiration stream to induce stomatal closure, however, maximum in vivo xylem sap Ca2+ concentrations of limed plants was only 1.7 mM and thus not high enough to influence stomata.We conclude that an alternative xylem-borne antitranspirant other than Ca2+ decreases gas exchange of limed plants.

AB - Liming is considered normal agricultural practise for remediating soil acidity and improving crop productivity; however recommended lime applications can reduce yield. We tested the hypothesis that elevated xylem sap Ca2+ limited gas exchange of Phaseolus vulgaris L. and Pisum sativum L. plants that exhibited reduced shoot biomass and leaf area when limed.We used Scholander and whole-plant pressure chamber techniques to collect root and leaf xylem sap, a calcium-specific ion-selective electrode to measure xylem sap Ca2+, infra-red gas analysis to measure gas exchange of limed and unlimed (control) plants, and a detached leaf transpiration bioassay to determine stomatal sensitivity to Ca2+.Liming reduced shoot biomass, leaf area and leaf gas exchange in both species. Root xylem sap Ca2+ concentration was only increased in P. vulgaris and not in P. sativum. Detached leaves of both species required 5 mM Ca2+ supplied to via the transpiration stream to induce stomatal closure, however, maximum in vivo xylem sap Ca2+ concentrations of limed plants was only 1.7 mM and thus not high enough to influence stomata.We conclude that an alternative xylem-borne antitranspirant other than Ca2+ decreases gas exchange of limed plants.

KW - Calcium

KW - Lime

KW - Pisum sativum

KW - Phaseolus vulgaris

KW - Stomatal conductance

KW - Xylem sap

KW - STOMATAL CONDUCTANCE

KW - ABSCISIC-ACID

KW - SOIL ACIDIFICATION

KW - RICINUS-COMMUNIS

KW - FLOW-RATE

KW - WATER

KW - PLANTS

KW - SHOOT

KW - LIME

KW - TRANSPIRATION

U2 - 10.1007/s11104-014-2118-5

DO - 10.1007/s11104-014-2118-5

M3 - Journal article

VL - 382

SP - 17

EP - 30

JO - Plant and Soil

JF - Plant and Soil

SN - 0032-079X

IS - 1-2

ER -

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