Guest Lecture from Dr Hari Shroff from the HHMI Janelia Farms, Ashburn, USA.
In this seminar, Hari surveyed key technologies developed in his lab, with an emphasis on microscopes well-suited for studying 4D cellular physiology. Advances in light-sheet and structured illumination microscopy were presented, including a recent multi-view confocal system that enables super-resolution imaging in tissue. He also discussed deep learning methods for further enhancing contrast, resolution, and experiment duration. Finally, he discussed the application of these methods to mapping nuclear positions in post-twitching embryonic C. elegans and combining this positional information with gene expression derived from single cell RNAseq experiments.