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    Rights statement: This is the author’s version of a work that was accepted for publication in Journal of Virological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Virological Methods, 260, 2018 DOI: 10.1016/j.jviromet.2018.07.009

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Production, characterization, and epitope mapping of a monoclonal antibody against genotype VII Newcastle disease virus V protein

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Production, characterization, and epitope mapping of a monoclonal antibody against genotype VII Newcastle disease virus V protein. / Li, Jihong; Meng, Chunchun; Ren, Tingting et al.
In: Journal of Virological Methods, Vol. 260, 01.10.2018, p. 88-97.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Li, J, Meng, C, Ren, T, Wang, W, Zhang, Y, Yuan, W, Xu, S, Sun, Y, Tan, L, Song, C, Liao, Y, Nair, V, Munir, M, Ding, Z, Liu, X, Qiu, X & Ding, C 2018, 'Production, characterization, and epitope mapping of a monoclonal antibody against genotype VII Newcastle disease virus V protein', Journal of Virological Methods, vol. 260, pp. 88-97. https://doi.org/10.1016/j.jviromet.2018.07.009

APA

Li, J., Meng, C., Ren, T., Wang, W., Zhang, Y., Yuan, W., Xu, S., Sun, Y., Tan, L., Song, C., Liao, Y., Nair, V., Munir, M., Ding, Z., Liu, X., Qiu, X., & Ding, C. (2018). Production, characterization, and epitope mapping of a monoclonal antibody against genotype VII Newcastle disease virus V protein. Journal of Virological Methods, 260, 88-97. https://doi.org/10.1016/j.jviromet.2018.07.009

Vancouver

Li J, Meng C, Ren T, Wang W, Zhang Y, Yuan W et al. Production, characterization, and epitope mapping of a monoclonal antibody against genotype VII Newcastle disease virus V protein. Journal of Virological Methods. 2018 Oct 1;260:88-97. Epub 2018 Jul 17. doi: 10.1016/j.jviromet.2018.07.009

Author

Li, Jihong ; Meng, Chunchun ; Ren, Tingting et al. / Production, characterization, and epitope mapping of a monoclonal antibody against genotype VII Newcastle disease virus V protein. In: Journal of Virological Methods. 2018 ; Vol. 260. pp. 88-97.

Bibtex

@article{decf4e30fe73481d8adb5205592c14b9,
title = "Production, characterization, and epitope mapping of a monoclonal antibody against genotype VII Newcastle disease virus V protein",
abstract = "Newcastle disease virus (NDV) V protein is crucial for viral interferon (IFN) antagonism and virulence, determining its host range restriction. However, little information is available on the B cell epitopes of V protein and the subcellular movement of V protein in the process of NDV infection. In this study, the monoclonal antibody (mAb) clone 3D7 against genotype VII NDV V protein was generated by immunizing mice with a purified recombinant His-tagged carboxyl-terminal domain (CTD) region of V protein. Fine epitope mapping analysis and B-cell epitope prediction indicated that mAb 3D7 recognized a linear epitope 152RGPAELWK159, which is located in the V protein CTD region. Sequence alignment showed that the mAb clone 3D7-recognized epitope is highly conserved among Class II genotype VII NDV strains, but not among other genotypes, suggesting it could serve as a genetic marker to differentiate NDV genotypes. Furthermore, the movement of V protein during NDV replication in infected cells were determined by using this mAb. It was found that V protein localized around the nucleus during virus replication. The establishment of V protein-specific mAb and identification of its epitope extend our understanding of the antigenic characteristics of V protein and provide a basis for the development of epitope-based diagnostic assays.",
keywords = "Newcastle disease virus, V protein, Monoclonal antibodies, Epitope",
author = "Jihong Li and Chunchun Meng and Tingting Ren and Wei Wang and Yaodan Zhang and Weifeng Yuan and Shuqin Xu and Yingjie Sun and Lei Tan and Cuiping Song and Ying Liao and Venugopal Nair and Muhammad Munir and Zhuang Ding and Xiufan Liu and Xusheng Qiu and Chan Ding",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Journal of Virological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Virological Methods, 260, 2018 DOI: 10.1016/j.jviromet.2018.07.009",
year = "2018",
month = oct,
day = "1",
doi = "10.1016/j.jviromet.2018.07.009",
language = "English",
volume = "260",
pages = "88--97",
journal = "Journal of Virological Methods",
issn = "0166-0934",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Production, characterization, and epitope mapping of a monoclonal antibody against genotype VII Newcastle disease virus V protein

AU - Li, Jihong

AU - Meng, Chunchun

AU - Ren, Tingting

AU - Wang, Wei

AU - Zhang, Yaodan

AU - Yuan, Weifeng

AU - Xu, Shuqin

AU - Sun, Yingjie

AU - Tan, Lei

AU - Song, Cuiping

AU - Liao, Ying

AU - Nair, Venugopal

AU - Munir, Muhammad

AU - Ding, Zhuang

AU - Liu, Xiufan

AU - Qiu, Xusheng

AU - Ding, Chan

N1 - This is the author’s version of a work that was accepted for publication in Journal of Virological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Virological Methods, 260, 2018 DOI: 10.1016/j.jviromet.2018.07.009

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Newcastle disease virus (NDV) V protein is crucial for viral interferon (IFN) antagonism and virulence, determining its host range restriction. However, little information is available on the B cell epitopes of V protein and the subcellular movement of V protein in the process of NDV infection. In this study, the monoclonal antibody (mAb) clone 3D7 against genotype VII NDV V protein was generated by immunizing mice with a purified recombinant His-tagged carboxyl-terminal domain (CTD) region of V protein. Fine epitope mapping analysis and B-cell epitope prediction indicated that mAb 3D7 recognized a linear epitope 152RGPAELWK159, which is located in the V protein CTD region. Sequence alignment showed that the mAb clone 3D7-recognized epitope is highly conserved among Class II genotype VII NDV strains, but not among other genotypes, suggesting it could serve as a genetic marker to differentiate NDV genotypes. Furthermore, the movement of V protein during NDV replication in infected cells were determined by using this mAb. It was found that V protein localized around the nucleus during virus replication. The establishment of V protein-specific mAb and identification of its epitope extend our understanding of the antigenic characteristics of V protein and provide a basis for the development of epitope-based diagnostic assays.

AB - Newcastle disease virus (NDV) V protein is crucial for viral interferon (IFN) antagonism and virulence, determining its host range restriction. However, little information is available on the B cell epitopes of V protein and the subcellular movement of V protein in the process of NDV infection. In this study, the monoclonal antibody (mAb) clone 3D7 against genotype VII NDV V protein was generated by immunizing mice with a purified recombinant His-tagged carboxyl-terminal domain (CTD) region of V protein. Fine epitope mapping analysis and B-cell epitope prediction indicated that mAb 3D7 recognized a linear epitope 152RGPAELWK159, which is located in the V protein CTD region. Sequence alignment showed that the mAb clone 3D7-recognized epitope is highly conserved among Class II genotype VII NDV strains, but not among other genotypes, suggesting it could serve as a genetic marker to differentiate NDV genotypes. Furthermore, the movement of V protein during NDV replication in infected cells were determined by using this mAb. It was found that V protein localized around the nucleus during virus replication. The establishment of V protein-specific mAb and identification of its epitope extend our understanding of the antigenic characteristics of V protein and provide a basis for the development of epitope-based diagnostic assays.

KW - Newcastle disease virus

KW - V protein

KW - Monoclonal antibodies

KW - Epitope

U2 - 10.1016/j.jviromet.2018.07.009

DO - 10.1016/j.jviromet.2018.07.009

M3 - Journal article

VL - 260

SP - 88

EP - 97

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

ER -