Targeted Knockdown of GDCH in Rice Leads to a Photorespiratory-Deficient Phenotype Useful as a Building Block for C4 Rice

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Targeted Knockdown of GDCH in Rice Leads to a Photorespiratory-Deficient Phenotype Useful as a Building Block for C4 Rice. / Lin, HsiangChun; Karki, Shanta; Coe, Robert A et al.
In: Plant and Cell Physiology, Vol. 57, No. 5, 30.05.2016, p. 919-32.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Lin, H, Karki, S, Coe, RA, Bagha, S, Khoshravesh, R, Balahadia, CP, Ver Sagun, J, Tapia, R, Israel, WK, Montecillo, F, de Luna, A, Danila, FR, Lazaro, A, Realubit, CM, Acoba, MG, Sage, TL, von Caemmerer, S, Furbank, RT, Cousins, AB, Hibberd, JM, Quick, WP & Covshoff, S 2016, 'Targeted Knockdown of GDCH in Rice Leads to a Photorespiratory-Deficient Phenotype Useful as a Building Block for C4 Rice', Plant and Cell Physiology, vol. 57, no. 5, pp. 919-32. https://doi.org/10.1093/pcp/pcw033

APA

Lin, H., Karki, S., Coe, R. A., Bagha, S., Khoshravesh, R., Balahadia, C. P., Ver Sagun, J., Tapia, R., Israel, W. K., Montecillo, F., de Luna, A., Danila, F. R., Lazaro, A., Realubit, C. M., Acoba, M. G., Sage, T. L., von Caemmerer, S., Furbank, R. T., Cousins, A. B., ... Covshoff, S. (2016). Targeted Knockdown of GDCH in Rice Leads to a Photorespiratory-Deficient Phenotype Useful as a Building Block for C4 Rice. Plant and Cell Physiology, 57(5), 919-32. https://doi.org/10.1093/pcp/pcw033

Vancouver

Lin H, Karki S, Coe RA, Bagha S, Khoshravesh R, Balahadia CP et al. Targeted Knockdown of GDCH in Rice Leads to a Photorespiratory-Deficient Phenotype Useful as a Building Block for C4 Rice. Plant and Cell Physiology. 2016 May 30;57(5):919-32. Epub 2016 Feb 21. doi: 10.1093/pcp/pcw033

Author

Lin, HsiangChun ; Karki, Shanta ; Coe, Robert A et al. / Targeted Knockdown of GDCH in Rice Leads to a Photorespiratory-Deficient Phenotype Useful as a Building Block for C4 Rice. In: Plant and Cell Physiology. 2016 ; Vol. 57, No. 5. pp. 919-32.

Bibtex

@article{014c368f4f81482c8569198145ffdac3,

title = "Targeted Knockdown of GDCH in Rice Leads to a Photorespiratory-Deficient Phenotype Useful as a Building Block for C4 Rice",

abstract = "The glycine decarboxylase complex (GDC) plays a critical role in the photorespiratory C2 cycle of C3 species by recovering carbon following the oxygenation reaction of ribulose-1,5-bisphosphate carboxylase/oxygenase. Loss of GDC from mesophyll cells (MCs) is considered a key early step in the evolution of C4 photosynthesis. To assess the impact of preferentially reducing GDC in rice MCs, we decreased the abundance of OsGDCH (Os10g37180) using an artificial microRNA (amiRNA) driven by a promoter that preferentially drives expression in MCs. GDC H- and P-proteins were undetectable in leaves of gdch lines. Plants exhibited a photorespiratory-deficient phenotype with stunted growth, accelerated leaf senescence, reduced chlorophyll, soluble protein and sugars, and increased glycine accumulation in leaves. Gas exchange measurements indicated an impaired ability to regenerate ribulose 1,5-bisphosphate in photorespiratory conditions. In addition, MCs of gdch lines exhibited a significant reduction in chloroplast area and coverage of the cell wall when grown in air, traits that occur during the later stages of C4 evolution. The presence of these two traits important for C4 photosynthesis and the non-lethal, down-regulation of the photorespiratory C2 cycle positively contribute to efforts to produce a C4 rice prototype. ",

keywords = "Carbon Cycle, Cell Respiration, Chloroplasts/metabolism, Gene Expression Regulation, Plant, Gene Knockdown Techniques, Glycine Decarboxylase Complex/genetics, Light, MicroRNAs/genetics, Oryza/enzymology, Phenotype, Photosynthesis, Plant Leaves/enzymology, Plant Proteins/genetics, Plants, Genetically Modified, Ribulose-Bisphosphate Carboxylase/genetics",

author = "HsiangChun Lin and Shanta Karki and Coe, {Robert A} and Shaheen Bagha and Roxana Khoshravesh and Balahadia, {C Paolo} and {Ver Sagun}, Julius and Ronald Tapia and Israel, {W Krystler} and Florencia Montecillo and {de Luna}, Albert and Danila, {Florence R} and Andrea Lazaro and Realubit, {Czarina M} and Acoba, {Michelle G} and Sage, {Tammy L} and {von Caemmerer}, Susanne and Furbank, {Robert T} and Cousins, {Asaph B} and Hibberd, {Julian M} and Quick, {W Paul} and Sarah Covshoff",

note = "{\textcopyright} The Author 2016. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.",

year = "2016",

month = may,

day = "30",

doi = "10.1093/pcp/pcw033",

language = "English",

volume = "57",

pages = "919--32",

journal = "Plant and Cell Physiology",

issn = "0032-0781",

publisher = "Oxford University Press",

number = "5",

}

RIS

TY - JOUR

T1 - Targeted Knockdown of GDCH in Rice Leads to a Photorespiratory-Deficient Phenotype Useful as a Building Block for C4 Rice

AU - Lin, HsiangChun

AU - Karki, Shanta

AU - Coe, Robert A

AU - Bagha, Shaheen

AU - Khoshravesh, Roxana

AU - Balahadia, C Paolo

AU - Ver Sagun, Julius

AU - Tapia, Ronald

AU - Israel, W Krystler

AU - Montecillo, Florencia

AU - de Luna, Albert

AU - Danila, Florence R

AU - Lazaro, Andrea

AU - Realubit, Czarina M

AU - Acoba, Michelle G

AU - Sage, Tammy L

AU - von Caemmerer, Susanne

AU - Furbank, Robert T

AU - Cousins, Asaph B

AU - Hibberd, Julian M

AU - Quick, W Paul

AU - Covshoff, Sarah

PY - 2016/5/30

Y1 - 2016/5/30

N2 - The glycine decarboxylase complex (GDC) plays a critical role in the photorespiratory C2 cycle of C3 species by recovering carbon following the oxygenation reaction of ribulose-1,5-bisphosphate carboxylase/oxygenase. Loss of GDC from mesophyll cells (MCs) is considered a key early step in the evolution of C4 photosynthesis. To assess the impact of preferentially reducing GDC in rice MCs, we decreased the abundance of OsGDCH (Os10g37180) using an artificial microRNA (amiRNA) driven by a promoter that preferentially drives expression in MCs. GDC H- and P-proteins were undetectable in leaves of gdch lines. Plants exhibited a photorespiratory-deficient phenotype with stunted growth, accelerated leaf senescence, reduced chlorophyll, soluble protein and sugars, and increased glycine accumulation in leaves. Gas exchange measurements indicated an impaired ability to regenerate ribulose 1,5-bisphosphate in photorespiratory conditions. In addition, MCs of gdch lines exhibited a significant reduction in chloroplast area and coverage of the cell wall when grown in air, traits that occur during the later stages of C4 evolution. The presence of these two traits important for C4 photosynthesis and the non-lethal, down-regulation of the photorespiratory C2 cycle positively contribute to efforts to produce a C4 rice prototype.

AB - The glycine decarboxylase complex (GDC) plays a critical role in the photorespiratory C2 cycle of C3 species by recovering carbon following the oxygenation reaction of ribulose-1,5-bisphosphate carboxylase/oxygenase. Loss of GDC from mesophyll cells (MCs) is considered a key early step in the evolution of C4 photosynthesis. To assess the impact of preferentially reducing GDC in rice MCs, we decreased the abundance of OsGDCH (Os10g37180) using an artificial microRNA (amiRNA) driven by a promoter that preferentially drives expression in MCs. GDC H- and P-proteins were undetectable in leaves of gdch lines. Plants exhibited a photorespiratory-deficient phenotype with stunted growth, accelerated leaf senescence, reduced chlorophyll, soluble protein and sugars, and increased glycine accumulation in leaves. Gas exchange measurements indicated an impaired ability to regenerate ribulose 1,5-bisphosphate in photorespiratory conditions. In addition, MCs of gdch lines exhibited a significant reduction in chloroplast area and coverage of the cell wall when grown in air, traits that occur during the later stages of C4 evolution. The presence of these two traits important for C4 photosynthesis and the non-lethal, down-regulation of the photorespiratory C2 cycle positively contribute to efforts to produce a C4 rice prototype.

KW - Carbon Cycle

KW - Cell Respiration

KW - Chloroplasts/metabolism

KW - Gene Expression Regulation, Plant

KW - Gene Knockdown Techniques

KW - Glycine Decarboxylase Complex/genetics

KW - Light

KW - MicroRNAs/genetics

KW - Oryza/enzymology

KW - Phenotype

KW - Photosynthesis

KW - Plant Leaves/enzymology

KW - Plant Proteins/genetics

KW - Plants, Genetically Modified

KW - Ribulose-Bisphosphate Carboxylase/genetics

U2 - 10.1093/pcp/pcw033

DO - 10.1093/pcp/pcw033

M3 - Journal article

C2 - 26903527

VL - 57

SP - 919

EP - 932

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 5

ER -

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