FastQCFastQC Report
Mon 15 Feb 2021
CUR_aber19_300_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCUR_aber19_300_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530062
Sequences flagged as poor quality0
Sequence length35-301
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC131240.5187224660897638TruSeq Adapter, Index 5 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC30800.12173614717742094TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)