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A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures

Research output: Contribution to journalJournal article

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  • A R Dluzewski
  • I T Ling
  • K Rangachari
  • P A Bates
  • R J Wilson
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<mark>Journal publication date</mark>1984
<mark>Journal</mark>Transactions of The Royal Society of Tropical Medicine and Hygiene
Issue number5
Volume78
Number of pages3
Pages (from-to)622-624
Publication StatusPublished
<mark>Original language</mark>English

Abstract

Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.