Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures
AU - Dluzewski, A R
AU - Ling, I T
AU - Rangachari, K
AU - Bates, P A
AU - Wilson, R J
PY - 1984
Y1 - 1984
N2 - Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.
AB - Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.
M3 - Journal article
C2 - 6095494
VL - 78
SP - 622
EP - 624
JO - Transactions of The Royal Society of Tropical Medicine and Hygiene
JF - Transactions of The Royal Society of Tropical Medicine and Hygiene
SN - 0035-9203
IS - 5
ER -