A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures

Biomedical and Life Sciences

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures. / Dluzewski, A R; Ling, I T; Rangachari, K et al.
In: Transactions of The Royal Society of Tropical Medicine and Hygiene, Vol. 78, No. 5, 1984, p. 622-624.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Dluzewski, AR, Ling, IT, Rangachari, K, Bates, PA & Wilson, RJ 1984, 'A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures', Transactions of The Royal Society of Tropical Medicine and Hygiene, vol. 78, no. 5, pp. 622-624. <http://www.tropicalmedandhygienejrnl.net/>

APA

Dluzewski, A. R., Ling, I. T., Rangachari, K., Bates, P. A., & Wilson, R. J. (1984). A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures. Transactions of The Royal Society of Tropical Medicine and Hygiene, 78(5), 622-624. http://www.tropicalmedandhygienejrnl.net/

Vancouver

Dluzewski AR, Ling IT, Rangachari K, Bates PA, Wilson RJ. A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures. Transactions of The Royal Society of Tropical Medicine and Hygiene. 1984;78(5):622-624.

Author

Dluzewski, A R ; Ling, I T ; Rangachari, K et al. / A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures. In: Transactions of The Royal Society of Tropical Medicine and Hygiene. 1984 ; Vol. 78, No. 5. pp. 622-624.

Bibtex

@article{49f524739eed4ef28535d74ccfb59022,

title = "A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures",

abstract = "Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.",

author = "Dluzewski, {A R} and Ling, {I T} and K Rangachari and Bates, {P A} and Wilson, {R J}",

year = "1984",

language = "English",

volume = "78",

pages = "622--624",

journal = "Transactions of The Royal Society of Tropical Medicine and Hygiene",

issn = "0035-9203",

publisher = "Oxford University Press Inc",

number = "5",

}

RIS

TY - JOUR

T1 - A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures

AU - Dluzewski, A R

AU - Ling, I T

AU - Rangachari, K

AU - Bates, P A

AU - Wilson, R J

PY - 1984

Y1 - 1984

N2 - Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.

AB - Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.

M3 - Journal article

C2 - 6095494

VL - 78

SP - 622

EP - 624

JO - Transactions of The Royal Society of Tropical Medicine and Hygiene

JF - Transactions of The Royal Society of Tropical Medicine and Hygiene

SN - 0035-9203

IS - 5

ER -

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