Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Application of a luminescence-based biosensor for assessing naphthalene biodegradation in soils from a manufactured gas plant.
AU - Paton, Graeme I.
AU - Reid, Brian J.
AU - Semple, Kirk T.
PY - 2009/5
Y1 - 2009/5
N2 - Despite numerous reviews suggesting that microbial biosensors could be used in many environmental applications, in reality they have failed to be used for which they were designed. In part this is because most of these sensors perform in an aqueous phase and a buffered medium, which is in contrast to the nature of genuine environmental systems. In this study, a range of non-exhaustive extraction techniques (NEETs) were assessed for (i) compatibility with a naphthalene responsive biosensor and (ii) correlation with naphthalene biodegradation. The NEETs removed a portion of the total soil naphthalene in the order of methanol > HPCD > βCD > water. To place the biosensor performance to NEETs in context, a biodegradation experiment was carried out using historically contaminated soils. By coupling the HPCD extraction with the biosensor, it was possible to assess the fraction of the naphthalene capable of undergoing microbial degradation in soil. Exposure of microbial biosensors to cyclodextrin solutions allows the assessment of the degradable fraction of contaminants in soil.
AB - Despite numerous reviews suggesting that microbial biosensors could be used in many environmental applications, in reality they have failed to be used for which they were designed. In part this is because most of these sensors perform in an aqueous phase and a buffered medium, which is in contrast to the nature of genuine environmental systems. In this study, a range of non-exhaustive extraction techniques (NEETs) were assessed for (i) compatibility with a naphthalene responsive biosensor and (ii) correlation with naphthalene biodegradation. The NEETs removed a portion of the total soil naphthalene in the order of methanol > HPCD > βCD > water. To place the biosensor performance to NEETs in context, a biodegradation experiment was carried out using historically contaminated soils. By coupling the HPCD extraction with the biosensor, it was possible to assess the fraction of the naphthalene capable of undergoing microbial degradation in soil. Exposure of microbial biosensors to cyclodextrin solutions allows the assessment of the degradable fraction of contaminants in soil.
KW - Bioluminescent bacteria
KW - Organic contaminants
KW - Biodegradation
KW - Extraction
KW - Bioavailability
U2 - 10.1016/j.envpol.2008.12.020
DO - 10.1016/j.envpol.2008.12.020
M3 - Journal article
VL - 157
SP - 1643
EP - 1648
JO - Environmental Pollution
JF - Environmental Pollution
SN - 0269-7491
IS - 5
ER -