Application of a luminescence-based biosensor for assessing naphthalene biodegradation in soils from a manufactured gas plant.

Lancaster Environment Centre

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https://doi.org/10.1016/j.envpol.2008.12.020
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Keywords

Bioluminescent bacteria, Organic contaminants, Biodegradation, Extraction, Bioavailability

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Application of a luminescence-based biosensor for assessing naphthalene biodegradation in soils from a manufactured gas plant. / Paton, Graeme I.; Reid, Brian J.; Semple, Kirk T.
In: Environmental Pollution, Vol. 157, No. 5, 05.2009, p. 1643-1648.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{883885eccc464259834937281d5a08ba,

title = "Application of a luminescence-based biosensor for assessing naphthalene biodegradation in soils from a manufactured gas plant.",

abstract = "Despite numerous reviews suggesting that microbial biosensors could be used in many environmental applications, in reality they have failed to be used for which they were designed. In part this is because most of these sensors perform in an aqueous phase and a buffered medium, which is in contrast to the nature of genuine environmental systems. In this study, a range of non-exhaustive extraction techniques (NEETs) were assessed for (i) compatibility with a naphthalene responsive biosensor and (ii) correlation with naphthalene biodegradation. The NEETs removed a portion of the total soil naphthalene in the order of methanol > HPCD > βCD > water. To place the biosensor performance to NEETs in context, a biodegradation experiment was carried out using historically contaminated soils. By coupling the HPCD extraction with the biosensor, it was possible to assess the fraction of the naphthalene capable of undergoing microbial degradation in soil. Exposure of microbial biosensors to cyclodextrin solutions allows the assessment of the degradable fraction of contaminants in soil.",

keywords = "Bioluminescent bacteria, Organic contaminants, Biodegradation, Extraction, Bioavailability",

author = "Paton, {Graeme I.} and Reid, {Brian J.} and Semple, {Kirk T.}",

year = "2009",

month = may,

doi = "10.1016/j.envpol.2008.12.020",

language = "English",

volume = "157",

pages = "1643--1648",

journal = "Environmental Pollution",

issn = "0269-7491",

publisher = "Elsevier Ltd",

number = "5",

}

RIS

TY - JOUR

T1 - Application of a luminescence-based biosensor for assessing naphthalene biodegradation in soils from a manufactured gas plant.

AU - Paton, Graeme I.

AU - Reid, Brian J.

AU - Semple, Kirk T.

PY - 2009/5

Y1 - 2009/5

N2 - Despite numerous reviews suggesting that microbial biosensors could be used in many environmental applications, in reality they have failed to be used for which they were designed. In part this is because most of these sensors perform in an aqueous phase and a buffered medium, which is in contrast to the nature of genuine environmental systems. In this study, a range of non-exhaustive extraction techniques (NEETs) were assessed for (i) compatibility with a naphthalene responsive biosensor and (ii) correlation with naphthalene biodegradation. The NEETs removed a portion of the total soil naphthalene in the order of methanol > HPCD > βCD > water. To place the biosensor performance to NEETs in context, a biodegradation experiment was carried out using historically contaminated soils. By coupling the HPCD extraction with the biosensor, it was possible to assess the fraction of the naphthalene capable of undergoing microbial degradation in soil. Exposure of microbial biosensors to cyclodextrin solutions allows the assessment of the degradable fraction of contaminants in soil.

AB - Despite numerous reviews suggesting that microbial biosensors could be used in many environmental applications, in reality they have failed to be used for which they were designed. In part this is because most of these sensors perform in an aqueous phase and a buffered medium, which is in contrast to the nature of genuine environmental systems. In this study, a range of non-exhaustive extraction techniques (NEETs) were assessed for (i) compatibility with a naphthalene responsive biosensor and (ii) correlation with naphthalene biodegradation. The NEETs removed a portion of the total soil naphthalene in the order of methanol > HPCD > βCD > water. To place the biosensor performance to NEETs in context, a biodegradation experiment was carried out using historically contaminated soils. By coupling the HPCD extraction with the biosensor, it was possible to assess the fraction of the naphthalene capable of undergoing microbial degradation in soil. Exposure of microbial biosensors to cyclodextrin solutions allows the assessment of the degradable fraction of contaminants in soil.

KW - Bioluminescent bacteria

KW - Organic contaminants

KW - Biodegradation

KW - Extraction

KW - Bioavailability

U2 - 10.1016/j.envpol.2008.12.020

DO - 10.1016/j.envpol.2008.12.020

M3 - Journal article

VL - 157

SP - 1643

EP - 1648

JO - Environmental Pollution

JF - Environmental Pollution

SN - 0269-7491

IS - 5

ER -

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Keywords