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Application of an asymmetric flow field flow fractionation multi-detector approach for metallic engineered nanoparticle characterization - Prospects and limitations demonstrated on Au nanoparticles

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Application of an asymmetric flow field flow fractionation multi-detector approach for metallic engineered nanoparticle characterization - Prospects and limitations demonstrated on Au nanoparticles. / Hagendorfer, H.; Kaegi, R.; Traber, J.; Mertens, S.F.; Scherrers, R.; Ludwig, C.; Ulrich, A.

In: Analytica Chimica Acta, Vol. 706, No. 2, 2011, p. 367-378.

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Hagendorfer, H. ; Kaegi, R. ; Traber, J. ; Mertens, S.F. ; Scherrers, R. ; Ludwig, C. ; Ulrich, A. / Application of an asymmetric flow field flow fractionation multi-detector approach for metallic engineered nanoparticle characterization - Prospects and limitations demonstrated on Au nanoparticles. In: Analytica Chimica Acta. 2011 ; Vol. 706, No. 2. pp. 367-378.

Bibtex

@article{10a9c17febde4806b6ea8f02c0d1583f,
title = "Application of an asymmetric flow field flow fractionation multi-detector approach for metallic engineered nanoparticle characterization - Prospects and limitations demonstrated on Au nanoparticles",
abstract = "In this work we discuss about the method development, applicability and limitations of an asymmetric flow field flow fractionation (A4F) system in combination with a multi-detector setup consisting of UV/vis, light scattering, and inductively coupled plasma mass spectrometry (ICPMS). The overall aim was to obtain a size dependent-, element specific-, and quantitative method appropriate for the characterization of metallic engineered nanoparticle (ENP) dispersions. Thus, systematic investigations of crucial method parameters were performed by employing well characterized Au nanoparticles (Au-NPs) as a defined model system. For good separation performance, the A4F flow-, membrane-, and carrier conditions were optimized. To obtain reliable size information, the use of laser light scattering based detectors was evaluated, where an online dynamic light scattering (DLS) detector showed good results for the investigated Au-NP up to a size of 80 nm in hydrodynamic diameter. To adapt large sensitivity differences of the various detectors, as well as to guarantee long term stability and minimum contamination of the mass spectrometer a split-flow concept for coupling ICPMS was evaluated. To test for reliable quantification, the ICPMS signal response of ionic Au standards was compared to that of Au-NP. Using proper stabilization with surfactants, no difference for concentrations of 1-50 μg Au L-1 in the size range from 5 to 80 nm for citrate stabilized dispersions was observed. However, studies using different A4F channel membranes showed unspecific particle-membrane interaction resulting in retention time shifts and unspecific loss of nanoparticles, depending on the Au-NP system as well as membrane batch and type. Thus, reliable quantification and discrimination of ionic and particular species was performed using ICPMS in combination with ultracentrifugation instead of direct quantification with the A4F multi-detector setup. Figures of merit were obtained, by comparing the results from the multi detector approach outlined above, with results from batch-DLS and transmission electron microscopy (TEM). Furthermore, validation performed with certified NIST Au-NP showed excellent agreement. The developed methods show potential for characterization of other commonly used and important metallic engineered nanoparticles. {\textcopyright} 2011 Elsevier B.V.",
keywords = "Asymmetric flow field flow fractionation, Au nanoparticles, ICPMS, Light scattering, Metallic engineered nanoparticles, Particle-membrane interaction, Characterization, Dispersions, Dynamic light scattering, Flow fields, Fractionation, Gold alloys, High resolution transmission electron microscopy, Inductively coupled plasma mass spectrometry, Liquid chromatography, Mass spectrometers, Mass spectrometry, Membranes, Metals, Nanoparticles, Transmission electron microscopy, Asymmetric-flow field flow fractionations, Au nanoparticle, Particle-membrane interactions, Gold, cellulose, gold nanoparticle, polystyrene, polyvinylidene fluoride, regenerated cellulose, surfactant, unclassified drug, article, artificial membrane, asymmetric flow field flow fractionation, controlled study, dispersion, dynamic light scattering, field flow fractionation, hydrophobicity, laser light scattering, light scattering, mass spectrometer, mass spectrometry, molecular stability, online analysis, particle size, priority journal, quantitative analysis, retention time, time, transmission electron microscopy, ultracentrifugation, ultraviolet spectroscopy, zeta potential",
author = "H. Hagendorfer and R. Kaegi and J. Traber and S.F. Mertens and R. Scherrers and C. Ludwig and A. Ulrich",
year = "2011",
doi = "10.1016/j.aca.2011.08.014",
language = "English",
volume = "706",
pages = "367--378",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier Science B.V.",
number = "2",

}

RIS

TY - JOUR

T1 - Application of an asymmetric flow field flow fractionation multi-detector approach for metallic engineered nanoparticle characterization - Prospects and limitations demonstrated on Au nanoparticles

AU - Hagendorfer, H.

AU - Kaegi, R.

AU - Traber, J.

AU - Mertens, S.F.

AU - Scherrers, R.

AU - Ludwig, C.

AU - Ulrich, A.

PY - 2011

Y1 - 2011

N2 - In this work we discuss about the method development, applicability and limitations of an asymmetric flow field flow fractionation (A4F) system in combination with a multi-detector setup consisting of UV/vis, light scattering, and inductively coupled plasma mass spectrometry (ICPMS). The overall aim was to obtain a size dependent-, element specific-, and quantitative method appropriate for the characterization of metallic engineered nanoparticle (ENP) dispersions. Thus, systematic investigations of crucial method parameters were performed by employing well characterized Au nanoparticles (Au-NPs) as a defined model system. For good separation performance, the A4F flow-, membrane-, and carrier conditions were optimized. To obtain reliable size information, the use of laser light scattering based detectors was evaluated, where an online dynamic light scattering (DLS) detector showed good results for the investigated Au-NP up to a size of 80 nm in hydrodynamic diameter. To adapt large sensitivity differences of the various detectors, as well as to guarantee long term stability and minimum contamination of the mass spectrometer a split-flow concept for coupling ICPMS was evaluated. To test for reliable quantification, the ICPMS signal response of ionic Au standards was compared to that of Au-NP. Using proper stabilization with surfactants, no difference for concentrations of 1-50 μg Au L-1 in the size range from 5 to 80 nm for citrate stabilized dispersions was observed. However, studies using different A4F channel membranes showed unspecific particle-membrane interaction resulting in retention time shifts and unspecific loss of nanoparticles, depending on the Au-NP system as well as membrane batch and type. Thus, reliable quantification and discrimination of ionic and particular species was performed using ICPMS in combination with ultracentrifugation instead of direct quantification with the A4F multi-detector setup. Figures of merit were obtained, by comparing the results from the multi detector approach outlined above, with results from batch-DLS and transmission electron microscopy (TEM). Furthermore, validation performed with certified NIST Au-NP showed excellent agreement. The developed methods show potential for characterization of other commonly used and important metallic engineered nanoparticles. © 2011 Elsevier B.V.

AB - In this work we discuss about the method development, applicability and limitations of an asymmetric flow field flow fractionation (A4F) system in combination with a multi-detector setup consisting of UV/vis, light scattering, and inductively coupled plasma mass spectrometry (ICPMS). The overall aim was to obtain a size dependent-, element specific-, and quantitative method appropriate for the characterization of metallic engineered nanoparticle (ENP) dispersions. Thus, systematic investigations of crucial method parameters were performed by employing well characterized Au nanoparticles (Au-NPs) as a defined model system. For good separation performance, the A4F flow-, membrane-, and carrier conditions were optimized. To obtain reliable size information, the use of laser light scattering based detectors was evaluated, where an online dynamic light scattering (DLS) detector showed good results for the investigated Au-NP up to a size of 80 nm in hydrodynamic diameter. To adapt large sensitivity differences of the various detectors, as well as to guarantee long term stability and minimum contamination of the mass spectrometer a split-flow concept for coupling ICPMS was evaluated. To test for reliable quantification, the ICPMS signal response of ionic Au standards was compared to that of Au-NP. Using proper stabilization with surfactants, no difference for concentrations of 1-50 μg Au L-1 in the size range from 5 to 80 nm for citrate stabilized dispersions was observed. However, studies using different A4F channel membranes showed unspecific particle-membrane interaction resulting in retention time shifts and unspecific loss of nanoparticles, depending on the Au-NP system as well as membrane batch and type. Thus, reliable quantification and discrimination of ionic and particular species was performed using ICPMS in combination with ultracentrifugation instead of direct quantification with the A4F multi-detector setup. Figures of merit were obtained, by comparing the results from the multi detector approach outlined above, with results from batch-DLS and transmission electron microscopy (TEM). Furthermore, validation performed with certified NIST Au-NP showed excellent agreement. The developed methods show potential for characterization of other commonly used and important metallic engineered nanoparticles. © 2011 Elsevier B.V.

KW - Asymmetric flow field flow fractionation

KW - Au nanoparticles

KW - ICPMS

KW - Light scattering

KW - Metallic engineered nanoparticles

KW - Particle-membrane interaction

KW - Characterization

KW - Dispersions

KW - Dynamic light scattering

KW - Flow fields

KW - Fractionation

KW - Gold alloys

KW - High resolution transmission electron microscopy

KW - Inductively coupled plasma mass spectrometry

KW - Liquid chromatography

KW - Mass spectrometers

KW - Mass spectrometry

KW - Membranes

KW - Metals

KW - Nanoparticles

KW - Transmission electron microscopy

KW - Asymmetric-flow field flow fractionations

KW - Au nanoparticle

KW - Particle-membrane interactions

KW - Gold

KW - cellulose

KW - gold nanoparticle

KW - polystyrene

KW - polyvinylidene fluoride

KW - regenerated cellulose

KW - surfactant

KW - unclassified drug

KW - article

KW - artificial membrane

KW - asymmetric flow field flow fractionation

KW - controlled study

KW - dispersion

KW - dynamic light scattering

KW - field flow fractionation

KW - hydrophobicity

KW - laser light scattering

KW - light scattering

KW - mass spectrometer

KW - mass spectrometry

KW - molecular stability

KW - online analysis

KW - particle size

KW - priority journal

KW - quantitative analysis

KW - retention time

KW - time

KW - transmission electron microscopy

KW - ultracentrifugation

KW - ultraviolet spectroscopy

KW - zeta potential

U2 - 10.1016/j.aca.2011.08.014

DO - 10.1016/j.aca.2011.08.014

M3 - Journal article

VL - 706

SP - 367

EP - 378

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

IS - 2

ER -