Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Application of an asymmetric flow field flow fractionation multi-detector approach for metallic engineered nanoparticle characterization - Prospects and limitations demonstrated on Au nanoparticles
AU - Hagendorfer, H.
AU - Kaegi, R.
AU - Traber, J.
AU - Mertens, S.F.
AU - Scherrers, R.
AU - Ludwig, C.
AU - Ulrich, A.
PY - 2011
Y1 - 2011
N2 - In this work we discuss about the method development, applicability and limitations of an asymmetric flow field flow fractionation (A4F) system in combination with a multi-detector setup consisting of UV/vis, light scattering, and inductively coupled plasma mass spectrometry (ICPMS). The overall aim was to obtain a size dependent-, element specific-, and quantitative method appropriate for the characterization of metallic engineered nanoparticle (ENP) dispersions. Thus, systematic investigations of crucial method parameters were performed by employing well characterized Au nanoparticles (Au-NPs) as a defined model system. For good separation performance, the A4F flow-, membrane-, and carrier conditions were optimized. To obtain reliable size information, the use of laser light scattering based detectors was evaluated, where an online dynamic light scattering (DLS) detector showed good results for the investigated Au-NP up to a size of 80 nm in hydrodynamic diameter. To adapt large sensitivity differences of the various detectors, as well as to guarantee long term stability and minimum contamination of the mass spectrometer a split-flow concept for coupling ICPMS was evaluated. To test for reliable quantification, the ICPMS signal response of ionic Au standards was compared to that of Au-NP. Using proper stabilization with surfactants, no difference for concentrations of 1-50 μg Au L-1 in the size range from 5 to 80 nm for citrate stabilized dispersions was observed. However, studies using different A4F channel membranes showed unspecific particle-membrane interaction resulting in retention time shifts and unspecific loss of nanoparticles, depending on the Au-NP system as well as membrane batch and type. Thus, reliable quantification and discrimination of ionic and particular species was performed using ICPMS in combination with ultracentrifugation instead of direct quantification with the A4F multi-detector setup. Figures of merit were obtained, by comparing the results from the multi detector approach outlined above, with results from batch-DLS and transmission electron microscopy (TEM). Furthermore, validation performed with certified NIST Au-NP showed excellent agreement. The developed methods show potential for characterization of other commonly used and important metallic engineered nanoparticles. © 2011 Elsevier B.V.
AB - In this work we discuss about the method development, applicability and limitations of an asymmetric flow field flow fractionation (A4F) system in combination with a multi-detector setup consisting of UV/vis, light scattering, and inductively coupled plasma mass spectrometry (ICPMS). The overall aim was to obtain a size dependent-, element specific-, and quantitative method appropriate for the characterization of metallic engineered nanoparticle (ENP) dispersions. Thus, systematic investigations of crucial method parameters were performed by employing well characterized Au nanoparticles (Au-NPs) as a defined model system. For good separation performance, the A4F flow-, membrane-, and carrier conditions were optimized. To obtain reliable size information, the use of laser light scattering based detectors was evaluated, where an online dynamic light scattering (DLS) detector showed good results for the investigated Au-NP up to a size of 80 nm in hydrodynamic diameter. To adapt large sensitivity differences of the various detectors, as well as to guarantee long term stability and minimum contamination of the mass spectrometer a split-flow concept for coupling ICPMS was evaluated. To test for reliable quantification, the ICPMS signal response of ionic Au standards was compared to that of Au-NP. Using proper stabilization with surfactants, no difference for concentrations of 1-50 μg Au L-1 in the size range from 5 to 80 nm for citrate stabilized dispersions was observed. However, studies using different A4F channel membranes showed unspecific particle-membrane interaction resulting in retention time shifts and unspecific loss of nanoparticles, depending on the Au-NP system as well as membrane batch and type. Thus, reliable quantification and discrimination of ionic and particular species was performed using ICPMS in combination with ultracentrifugation instead of direct quantification with the A4F multi-detector setup. Figures of merit were obtained, by comparing the results from the multi detector approach outlined above, with results from batch-DLS and transmission electron microscopy (TEM). Furthermore, validation performed with certified NIST Au-NP showed excellent agreement. The developed methods show potential for characterization of other commonly used and important metallic engineered nanoparticles. © 2011 Elsevier B.V.
KW - Asymmetric flow field flow fractionation
KW - Au nanoparticles
KW - ICPMS
KW - Light scattering
KW - Metallic engineered nanoparticles
KW - Particle-membrane interaction
KW - Characterization
KW - Dispersions
KW - Dynamic light scattering
KW - Flow fields
KW - Fractionation
KW - Gold alloys
KW - High resolution transmission electron microscopy
KW - Inductively coupled plasma mass spectrometry
KW - Liquid chromatography
KW - Mass spectrometers
KW - Mass spectrometry
KW - Membranes
KW - Metals
KW - Nanoparticles
KW - Transmission electron microscopy
KW - Asymmetric-flow field flow fractionations
KW - Au nanoparticle
KW - Particle-membrane interactions
KW - Gold
KW - cellulose
KW - gold nanoparticle
KW - polystyrene
KW - polyvinylidene fluoride
KW - regenerated cellulose
KW - surfactant
KW - unclassified drug
KW - article
KW - artificial membrane
KW - asymmetric flow field flow fractionation
KW - controlled study
KW - dispersion
KW - dynamic light scattering
KW - field flow fractionation
KW - hydrophobicity
KW - laser light scattering
KW - light scattering
KW - mass spectrometer
KW - mass spectrometry
KW - molecular stability
KW - online analysis
KW - particle size
KW - priority journal
KW - quantitative analysis
KW - retention time
KW - time
KW - transmission electron microscopy
KW - ultracentrifugation
KW - ultraviolet spectroscopy
KW - zeta potential
U2 - 10.1016/j.aca.2011.08.014
DO - 10.1016/j.aca.2011.08.014
M3 - Journal article
VL - 706
SP - 367
EP - 378
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 2
ER -